LOCUS       sequence-446713-        9627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mini-Tn7 dCas9 expression vector - XylS/pM promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Roghair Stroud MN, Vang DX, Halverson LJ
  TITLE     Optimized CRISPR Interference System for Investigating Pseudomonas 
            alloputida Genes Involved in Rhizosphere Microbiome Assembly.
  JOURNAL   ACS Synth Biol. 2024 Aug 20. doi: 10.1021/acssynbio.4c00312.
  PUBMED    39163848
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Aug 20. doi: 10.1021/acssynbio.4c00312."
COMMENT     Sequence Label: pMRS-XylS-dCas9
FEATURES             Location/Qualifiers
     source          1..9627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(61..1026)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        1926..2006
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     RBS             2112..2120
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      6282..6353
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(6356..6372)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6356..6372)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      6415..6509
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(6811..6830)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6822..7637
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            7804..7912
                     /note="incP origin of transfer"
     rep_origin      7934..8322
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     mobile_element  8331..8496
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     terminator      complement(8510..8596)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    8631..8678
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(8808..9341)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(9511..9516,9517..9533,9534..9539))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    9580..9627
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 agagcgcttt tgaagctaat tcgatcatgc atgagcgacg tcttaattaa acgttcgtaa
       61 tcaagccact tcctttttgc attgacgcag ggtgtcggaa ggcaactcgc cgaacgcgct
      121 cctatagttt tcagcgaagc gtcccaaatg taagaagccg tagtctaggg ctatctcagt
      181 tatactacgc acattggcac tgggatcgtt caagcaggcg cggatgcttt cgagcttgcg
      241 gttgcggatg tagttcttcg gcgtggtgcc ggcgtgcttc tcgaacaaat tgtagagcga
      301 gcgtggactc atcatcgcca gctccgctaa ccgctcaagg ctgatattcc gtttgagatt
      361 ctcctcaatg aattgaacga ctcgctcgaa agacgggtta cctttgctga aaatttcacg
      421 gctgacattg ctgcccagca tttcgagcag cttggaagcg atgatccccg catagtgctc
      481 ttggacccga ggcatcgact ttgtatgttc cgcttcgtca caaactaacc cgagtagatt
      541 gataaagcca tcgagttgct ggagattgtg tcgcgcggcg aaacggatac cctccctcgg
      601 cttgtgccaa ttgttgtcac tgcacgcccg atcaaggacc actgagggca atttaacgat
      661 aaatttctcg caatcttctg aataggtcag gtcggcttgg tcatccggat tgagcagcaa
      721 tagttcgccc ggcgcaaaat agtgctcctg gccatggcca cgccacaggc aatggccttt
      781 gagtattatt tgcagatgat aacaggtctc taatccaggc gagattaccc tcacgctacc
      841 gccgtagctg attcgacaca gatcgaggca tccgaagatt ctgtggtgca gcctgcctgc
      901 cgggcgcccg cccttgggca ggcgaataga gtgcgtaccg acatactggt taacataatc
      961 ggagactgca tagggctcgg cgtggacgaa gatctgactt ttctcgttca ataagcaaaa
     1021 atccatagtt cacggttctc ttattttaat gtgggctgct tggtgtgatg tagaaaggcg
     1081 ccaagtcgat gaaaatgcat ctcgacgtga tgcgtatacg ggttaccccc attgccacgt
     1141 tgcgccatcc tttttgcaat cagtgaccac ttttccaagc aaaaataacg ccaagcagaa
     1201 cgaagacgtt ctttttaaga agcgagaaca ccagaagttc gtgctgtcgg ggcatggggc
     1261 gacgaattgg cggataaagg ggatctgctg gatattacgg cctttttaaa gaccgtaaag
     1321 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     1381 catccgtaat tacgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     1441 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     1501 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     1561 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     1621 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     1681 gttttcacca tgcatgggaa ttagcttgat ctgaccaacg accggtagcg gagctatcca
     1741 acggcggtat accaggaaaa cacacagcag gtacatcaga acagtaccat gactgaagaa
     1801 caaatagttt tttcctgatc cataaagcag aacggcctgc tccatgacaa atctggctcc
     1861 ccaactaatg ccccatgcag ccagcataac cagcataaag tgcagtgtcc ggtttgatag
     1921 ggataagtcc agccttgcaa gaagcggata caggagtgca aaaaatggct atctctagta
     1981 aggcctaccc cttaggcttt atgcaacaga aacaataata atggagtcat gaccatgcct
     2041 agggcccaag ttcacttaaa aaggagatca acaatgaaag caattttcgt actgaaacat
     2101 cttaatcatg ctaaggaggt accgctagcg gttatagaag gagatataca tatggataaa
     2161 aagtacagca tcgggctcgc gatcgggacc aactccgtgg ggtgggccgt catcacggac
     2221 gagtataagg tcccgtcgaa gaaattcaag gtcctcggga acacggatcg gcatagcatc
     2281 aaaaaagacc tgatcggggc cctcctgttc gactccggcg agaccgcgga ggcgacgcgc
     2341 ctcaagcgca cggcccggcg ccgctatacg cggcgcaaaa atcggatctg ctatctccaa
     2401 gagatcttca gcaatgagat ggcgaaagtg gatgactcct tcttccaccg gctcgaagag
     2461 tcgttcctgg tcgaggaaga taaaaagcat gagagccatc cgatcttcgg gaatatcgtg
     2521 gacgaggtgg cctaccatga gaaatacccg accatctatc atctgcggaa gaaactggtg
     2581 gactccaccg acaaggcgga cctccgcctg atctatctcg cgctggccca tatgatcaag
     2641 ttccgcgggc acttcctgat cgagggggat ctcaaccccg ataactcgga tgtggacaaa
     2701 ctgttcatcc aactcgtcca gacctataat caactgttcg aagagaatcc gatcaatgcc
     2761 agcggcgtcg atgccaaggc gatcctctcg gcccggctct ccaagtcgcg gcggctcgaa
     2821 aatctgatcg cccaactccc cggcgagaag aagaatggcc tgttcgggaa cctcatcgcc
     2881 ctctccctgg ggctgacccc caatttcaaa agcaacttcg acctggccga agatgcgaaa
     2941 ctccagctgt ccaaggatac ctacgacgac gatctggaca acctcctggc gcaaatcggg
     3001 gatcagtatg ccgatctctt cctggcggcc aaaaatctct ccgatgcgat cctcctgtcg
     3061 gatatcctgc gggtgaatac cgagatcacc aaagcccccc tgagcgcctc gatgatcaaa
     3121 cgctacgacg agcatcatca agacctgacc ctcctgaagg ccctcgtgcg ccagcagctg
     3181 ccggagaaat ataaagaaat cttcttcgat cagtccaaaa atgggtatgc ggggtacatc
     3241 gacggcgggg cctcgcagga agagttctat aagttcatca aaccgatcct ggaaaaaatg
     3301 gatgggaccg aagagctcct ggtgaaactc aaccgcgaag acctcctgcg gaaacagcgc
     3361 acgttcgaca acggctccat cccccatcaa atccacctcg gggagctcca tgcgatcctc
     3421 cgccggcagg aagatttcta ccccttcctc aaagataacc gggaaaaaat cgagaaaatc
     3481 ctcaccttcc ggatccccta ctacgtcggc ccgctggcgc gggggaactc ccggttcgcg
     3541 tggatgacgc gcaagtcgga ggagacgatc accccctgga atttcgaaga ggtggtggat
     3601 aagggcgcct cggcgcaaag cttcatcgaa cggatgacga atttcgacaa aaacctcccg
     3661 aatgagaaag tcctgcccaa gcattcgctg ctctacgaat atttcaccgt gtacaatgaa
     3721 ctgacgaaag tcaagtatgt caccgagggc atgcgcaaac ccgccttcct gtcgggggaa
     3781 cagaaaaagg cgatcgtgga tctgctgttc aaaaccaatc gcaaggtcac ggtgaagcag
     3841 ctcaaagaag actatttcaa aaaaatcgag tgcttcgatt ccgtggagat ctccggcgtg
     3901 gaagatcggt tcaacgcgtc gctggggacc tatcatgatc tgctgaaaat catcaaagat
     3961 aaggacttcc tggacaacga agaaaatgaa gacatcctgg aggacatcgt cctcacgctg
     4021 acgctgttcg aggatcgcga gatgatcgaa gagcgactga agacgtatgc ccatctcttc
     4081 gacgataaag tgatgaaaca actcaaacgc cggcggtaca cgggctgggg gcgcctgtcc
     4141 cgcaagctga tcaacgggat ccgggacaaa caaagtggga aaacgatcct cgacttcctc
     4201 aagtcggatg gcttcgcgaa ccggaacttc atgcaactca tccatgacga cagcctcacc
     4261 ttcaaggaag atatccaaaa agcccaagtc tccggccagg gcgactccct gcacgaacat
     4321 atcgcgaacc tggcgggctc gcccgccatc aagaaaggca tcctccagac ggtcaaagtg
     4381 gtcgacgaac tggtgaaagt gatggggcgg cataaacccg agaatatcgt catcgaaatg
     4441 gcccgcgaga accaaaccac gcagaaaggc cagaaaaata gccgggagcg catgaagcgc
     4501 atcgaggaag gcatcaagga gctgggcagc cagatcctca aggagcatcc ggtcgaaaac
     4561 acccaactcc aaaatgagaa gctgtatctc tactatctgc aaaatgggcg cgatatgtac
     4621 gtcgatcagg agctcgatat caaccgcctc tccgactacg atgtggatgc gatcgtcccc
     4681 cagtccttcc tgaaagatga cagcatcgat aacaaggtgc tcacccggtc ggacaaaaat
     4741 cgcgggaagt ccgataatgt cccgtccgaa gaagtggtga aaaagatgaa gaactactgg
     4801 cggcaactcc tcaacgcgaa gctgatcacc cagcgcaaat tcgataacct caccaaggcc
     4861 gagcggggcg ggctctcgga gctcgataag gccggcttca tcaagcgcca gctggtcgag
     4921 acgcgccaaa tcacgaagca cgtggcgcaa atcctcgata gccggatgaa taccaagtat
     4981 gacgagaatg ataaactcat ccgggaagtg aaagtgatca cgctgaaaag caaactcgtg
     5041 tccgacttcc ggaaggattt ccaattctat aaagtgcgcg aaatcaacaa ctatcatcac
     5101 gcgcatgacg cgtacctcaa tgccgtcgtg ggcacggccc tcatcaagaa gtacccgaag
     5161 ctggaaagcg aattcgtcta cggcgattac aaagtgtacg atgtgcgcaa aatgatcgcg
     5221 aaatcggaac aagaaatcgg caaggcgacc gcgaagtact tcttctattc gaatatcatg
     5281 aatttcttca aaaccgaaat caccctcgcg aacggcgaaa tccgcaaacg cccgctcatc
     5341 gagacgaacg gggagaccgg ggaaatcgtg tgggacaagg ggcgcgactt cgccaccgtg
     5401 cggaaagtcc tgtccatgcc gcaagtgaat atcgtgaaaa aaaccgaagt gcaaaccggc
     5461 ggcttctcga aggaaagcat cctccccaaa cgcaacagcg ataaactgat cgcccggaag
     5521 aaggactggg accccaaaaa atatgggggc ttcgactcgc cgaccgtggc gtactcggtc
     5581 ctcgtggtgg cgaaggtcga aaaagggaaa agcaaaaaac tgaaaagcgt caaggaactg
     5641 ctcggcatca cgatcatgga gcgctcctcc ttcgaaaaaa acccgatcga tttcctggag
     5701 gcgaaaggct acaaggaggt caaaaaggac ctgatcatca agctccccaa atatagcctg
     5761 ttcgaactgg aaaatggccg caagcggatg ctggcctccg cgggcgagct gcaaaagggg
     5821 aacgaactcg cgctcccgag caaatacgtc aacttcctct atctcgcgtc gcattatgaa
     5881 aagctgaagg ggagccccga atataatgaa caaaaacagc tgttcgtgga acaacataaa
     5941 cactatctcg atgagatcat cgagcaaatc tcggagttct ccaaacgggt catcctcgcg
     6001 gacgccaatc tcgataaggt gctgtcggcg tacaataaac atcgcgataa gccgatccgc
     6061 gagcaagccg agaacatcat ccacctcttc acgctgacga atctgggcgc gccggccgcc
     6121 ttcaagtact tcgacacgac gatcgatcgg aagcggtaca cctcgacgaa agaagtgctc
     6181 gacgccacgc tgatccacca atccatcacg ggtctgtatg aaacccgcat cgacctctcg
     6241 caactcggcg gggactaact cgagtaagga tctccaggca tcaaataaaa cgaaaggctc
     6301 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcgggatcc
     6361 actagttcta gagcggccgc gtcgtgactg ggaaaaccct ggcgactagt cttggactcc
     6421 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
     6481 gccgccgggc gttttttatt ggtgagaatc cagccacgcc cctctttaat acgacgggca
     6541 atttgcactt cagaaaatga agagtttgct ttagccataa caaaagtcca gtatgctttt
     6601 tcacagcata actggactga tttcagttta caactattct gtctagttta agactttatt
     6661 gtcatagttt agatctattt tgttcagttt aagactttat tgtccgccca caatttaaat
     6721 ttgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     6781 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcagcgtg
     6841 aaacgagctg tagccgtccg cgtctgaaca gcaacatgga tgcggatctg tatggctata
     6901 aatgggcgcg tgataacgtg ggtcagagcg gcgcgaccat ttatcgtctg tatggcaaac
     6961 cggatgcgcc ggaactgttt ctgaaacatg gcaaaggcag cgtggcgaac gatgtgaccg
     7021 atgaaatggt gcgtctgaac tggctgaccg aatttatgcc gctgccgacc attaaacatt
     7081 ttattcgcac cccggatgat gcgtggctgc tgaccaccgc gattccgggc aaaaccgcgt
     7141 ttcaggtgct ggaagaatat ccggatagcg gcgaaaacat tgtggatgcg ctggccgtgt
     7201 ttctgcgtcg tctgcatagc attccggtgt gcaactgccc gtttaacagc gatcgtgtgt
     7261 ttcgtctggc ccaggcgcag agccgtatga acaacggcct ggtggatgcg agcgattttg
     7321 atgatgaacg taacggctgg ccggtggaac aggtgtggaa agaaatgcat aaactgctgc
     7381 cgtttagccc ggatagcgtg gtgacccacg gcgattttag cctggataac ctgattttcg
     7441 atgaaggcaa actgattggc tgcattgatg tgggccgtgt gggcattgcg gatcgttatc
     7501 aggatctggc cattctgtgg aactgcctgg gcgaatttag cccgagcctg caaaaacgtc
     7561 tgtttcagaa atatggcatt gataatccgg atatgaacaa actgcaattt catctgatgc
     7621 tggatgaatt tttctaataa ttaattggac cgcggtccgc gcgttgtcct tttccgctgc
     7681 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
     7741 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
     7801 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
     7861 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg
     7921 ccggcgcgcc ccatgtcagc cgttaagtgt tcctgtgtca ctcaaaattg ctttgagagg
     7981 ctctaagggc ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta
     8041 aaggctttaa aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct
     8101 atttaagttg ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa
     8161 catgagagct tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc
     8221 gttaaacatg agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct
     8281 tagtacgtac tatcaacagg ttgaactgct gatcttcaga tcggcgcgcc tgtgggcgga
     8341 caaaatagtt gggaactggg aggggtggaa atggagtttt taaggattat ttagggaaga
     8401 gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct aatacgaaaa ttaaaaatga
     8461 caaaatagtt tggaactaga tttcacttat ctggtttcta gggcggcgga tttgtcctac
     8521 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     8581 cctttcgttt tatttgatgc cgacgtccgg acgatcgaat tggggatctt gaagtaccta
     8641 ttccgaagtt cctattctct agaaagtata ggaacttcag agcgcttttg aagctgatgt
     8701 gcttaaaaac ttactcaatg gaattctaga tcgaattggc cgcggcgttg tgacaattta
     8761 ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta
     8821 cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc
     8881 actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg
     8941 gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg
     9001 gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt gggcagaacg
     9061 taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc
     9121 ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct
     9181 acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca
     9241 gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg
     9301 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
     9361 taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa
     9421 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
     9481 ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaat
     9541 ccgatccatt gctgttgaca aagggaatca ggggatcttg aagttcctat tccgaagttc
     9601 ctattctcta gaaagtatag gaacttc
//
