LOCUS       sequence-44674-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Slavoff SA, Liu DS, Cohen JD, Ting AY
  TITLE     Imaging Protein-Protein Interactions inside Living Cells via 
            Interaction-Dependent Fluorophore Ligation.
  JOURNAL   J Am Chem Soc. 2011 Dec 14;133(49):19769-76. Epub 2011 Nov 18.
  PUBMED    22098454
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc."; date: "2011-12-14"; volume: "133(49)"; pages: "19769-76. Epub
            2011 Nov 18"
COMMENT     Sequence Label: pcDNA3-FRB-LplA(W37V)
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             124..150
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             451..474
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttgcgg ccgccaccat ggtgcggggt tctcatcatc atcatcatca tggtatggct
      121 agctatccgt acgacgtacc agactacgca atgtggcatg aaggcctgga agaggcatct
      181 cgtttgtact ttggggaaag gaacgtgaaa ggcatgtttg aggtgctgga gcccttgcat
      241 gctatgatgg aacggggccc ccagactctg aaggaaacat cctttaatca ggcctatggt
      301 cgagatttaa tggaggccca agagtggtgc aggaagtaca tgaaatcagg gaatgtcaag
      361 gacctcaccc aagcctggga cctctattat catgtgttcc gacgaatctc aaagggctcg
      421 ggctcgacct cgggctcggg caaggatcca gactacaagg atgacgacga taagatgagc
      481 accctgagac tgctgatcag cgacagctac gacccctggt tcaacctggc cgtggaggag
      541 tgcatcttca gacagatgcc cgccacccag agagtgctgt tcctggtgag aaacgccgac
      601 accgtggtga tcggcagagc ccagaacccc tggaaggagt gcaacaccag aagaatggag
      661 gaggacaacg tgagactggc cagaagaagc agcggcggcg gcgccgtgtt ccacgacctg
      721 ggcaacacct gcttcacctt catggccggc aagcccgagt acgacaagac catcagcacc
      781 agcatcgtgc tgaacgccct gaacgccctg ggcgtgagcg ccgaggccag cggcagaaac
      841 gacctggtgg tgaagaccgt ggagggcgac agaaaggtga gcggcagcgc ctacagagag
      901 ac
//
