LOCUS       sequence-44676-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V
  TITLE     A retro-lentiviral system for doxycycline-inducible gene expression 
            and gene knockdown in cells with limited proliferative capacity.
  JOURNAL   Mol Biol Rep. 2010 Apr . 37(4):1987-91.
  PUBMED    19655272
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Rep. 2010 Apr . 37(4):1987-91."
COMMENT     Sequence Label: pMA3160
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      75..84
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(147..165)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gcgattccag cacagtggcg gccgctcgag atctgcgatc taagtaagct tggcattccg
       61 gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat
      121 tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg
      181 ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg
      241 ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata
      301 caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg
      361 gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat
      421 tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc
      481 aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt atcatggatt
      541 ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc
      601 ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag acaattgcac
      661 tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg cctcatagaa
      721 ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg
      781 atactgcgat tttaagtgtt gttccattcc atcacggttt tggcatgtt
//
