LOCUS       sequence-446763-        2965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ColA BBa_B0014 tetAp SD8 ScTrpRS-[ScTrpRS-H15] T3Te-T7Te DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Costello A, Peterson AA, Lanster DL, Li Z, Carver GD, Badran AH
  TITLE     Efficient genetic code expansion without host genome modifications.
  JOURNAL   Nat Biotechnol. 2024 Sep 11. doi: 10.1038/s41587-024-02385-y.
  PUBMED    39261591
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Sep 11. doi: 10.1038/s41587-024-02385-y."
COMMENT     Sequence Label: pAB228v6
FEATURES             Location/Qualifiers
     source          1..2965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..636)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(146..165)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      659..686
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        788..843
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    794..812
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    824..842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      2227..2256
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      complement(2257..2284)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 aaacgtccta gaagatgcca ggaggatact tagcagagag acaataaggc cggagcgaag
       61 ccgtttttcc ataggctccg cccccctgac gaacatcacg aaatctgacg ctcaaatcag
      121 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc
      181 ttgcgctctc ctgttcccgt cctgcggcgt ccgtgttgtg gtggaggctt tacccaaatc
      241 accacgtccc gttccgtgta gacagttcgc tccaagctgg gctgtgtgca agaacccccc
      301 gttcagcccg actgctgcgc cttatccggt aactatcatc ttgagtccaa cccggaaaga
      361 cacgacaaaa cgccactggc agcagccatt ggtaactgag aattagtgga tttagatatc
      421 gagagtcttg aagtggtggc ctaacagagg ctacactgaa aggacagtat ttggtatctg
      481 cgctccacta aagccagtta ccaggttaag cagttcccca actgacttaa ccttcgatca
      541 aaccgcctcc ccaggcggtt ttttcgttta cagagcagga gattacgacg atcgtaaaag
      601 gatctcaaga agatcattta cggattcccg acaccaaaga ggacatccgg ttcacactgg
      661 ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga
      721 ttattaatcc ggctttttta ttatttagac ttagggaccc tgcggccgcc attaattcct
      781 aatttttgtt gacactctat cattgataga gttattttac cactccctat cagtgataga
      841 gaaaagtgaa atgaatagac ctgcaggtgc agtaaggagg aaaaaaaaat gtctaacgat
      901 gaaaccgttg aaaaagttac ccagcaggtt tctgaattga aatctaccga tgttaaagaa
      961 caggttgtta ccccgtggga tgttgaaggt ggtgttgatg aacagggtcg tgcgcagaac
     1021 attgattacg ataaattgat taaacagttc ggtaccaaac cggttaacga agaaaccttg
     1081 aaacgtttca aacaggttac cggtcgtgaa ccgcatcatt tcttgcgtaa aggtttgttc
     1141 ttctctgaac gtgatttcac caaaattttg gatttgtacg aacagggtaa accgttcttc
     1201 ttggttaccg gtcgtggtcc gtcttctgat tctatgcatt tgggtcatat gattccgttc
     1261 gttttcacca aatggttgca ggaagttttc gatgttccgt tggttattcc gttgaccgat
     1321 gatgaaaaat tcttgttcaa acataaattg accattaacg atgttaaaaa cttcgcgcgt
     1381 gaaaacgcga aagatattat tgcggttggt ttcgatccga aaaacacctt cattttctct
     1441 gatttgcagt acatgggtgg tgcgttctac gaaaccgttg ttcgtgtttc tcgtcagatt
     1501 accggttcta ccgcgaaagc ggttttcggt ttcaacgatt ctgattgtat tggtaaattc
     1561 catttcgcgt ctattcagat tgcgtgtgcg ttcccgtctt ctttcccgaa cgttttgggt
     1621 ttgccggata aaaccccgtg tttgtgtccg tgtgcgattg atcaggatcc gtacttccgt
     1681 gtttgtcgtg atgttgcgga taaattgaaa tactctaaac cggcgttgtt gcattctcgt
     1741 ttcttcccgg cgttgcaggg ttctaccacc aaaatgtctg cgtctgatga taccaccgcg
     1801 attttcatga ccgatacccc gaaacagatt cagaaaaaaa ttaacaaata cgcgttctct
     1861 ggtggtcagg tttctgcgga tttgcatcgt gaattgggtg gtaacccgga tgttgatgtt
     1921 gcgtaccagt acttgtcttt cttcaaagat gatgatgttt tcttgaaaga atgttacgat
     1981 aaatacaaat ctggtgaatt gttgtctggt gaaatgaaaa aattgtgtat tgaaaccttg
     2041 caggaattcg ttaaagcgtt ccaggaacgt cgtgcgcagg ttgatgaaga aaccttggat
     2101 aaattcatgg ttccgcataa attggtttgg ggtgaaaaag aacgtttggt tgcgccgaaa
     2161 ccgaaaacca aacaggaaaa aaaataaact taattaacgg cactcctcag ctgtaaccaa
     2221 ctcactggct caccttcacg ggtgagcctt tcttcgcgca gaaaggccca cccgaaggtg
     2281 agccagtgtg attacattta tgcccatggc gtttaacctt tctgccaaat ctggtaagag
     2341 tagttaatgt tagacgcgaa atcctgggtg aaaaccggac ggaagttaga cggaatttcc
     2401 gggaagtaaa catcaccttc cggttcaata tcaatggtag aaatatgcaa ggtatcaacc
     2461 tgatcaatca aagatttgta aatttcacca ccaccagaaa caataacatg atcggtaatt
     2521 tttttcaagt tggtcaacgc atctttaata gacgggaaaa tcaaaacgtt ttcgttatca
     2581 gaggtgaaag aagaacgggt aacaaccgcg tatttacggt tcggcaacgc acccatagaa
     2641 ccgaaggttt tacgaccaac caacaaccac tggttgtagg taatcgcttt gaacaacaac
     2701 tgttcacctt tcgcagacca cggaatatcc ggaccgttac caataacacc gtttttagaa
     2761 atcgcaacca tcaaagacaa tttcataatt cttcctcaga ggttaacatt ttattagtaa
     2821 gcatgctcgt tttgtcaccc gctgatgctt accgttaatt aatataatca cttgtattaa
     2881 atagactttc tctaaaaata caagacactc tgttattaca aatcgtgcgt gccgtctatc
     2941 ctgcttgcaa ggccaaatag gccgt
//
