LOCUS       sequence-44678-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: TALE8
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..61)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(83..232)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(257..277)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgctcactgg gccaggattc tcctcgacgt caccgcatgt tagcagactt cctctgccct
       61 ctccactgcc tctagagtta atcagcatgt ccaggtcgaa atcatcaagg gcgtcactgc
      121 cgagcatgtc gaggtcaaag tcatcaaggg catccgaacc aagcatgtca aggtcaaaat
      181 catcgagggc gtcacttccc agcatatcca gatcaaaatc gtccaatgcg tcagcccgtc
      241 cggaaccgct ggcctccacc tttctcttct tcttggggct agctgacgcg cgagtttggt
      301 ccccttcatg catgggcgag ggcgcgtcca aatccctctc cagcgagtct gcgaacgcat
      361 gaaggctcgc ctggtcaggt gtctgagttg acgtaggtga aggcttcgcg cgtttcatac
      421 cgctcgcttg gagaatcctg tcccaccgct gggaggcggg aggcagtgtg cccgagcggg
      481 cctcgagttc tgtgacaccg actctacgaa agagctgcag cagtccgtgt ctcgacatac
      541 caaattgagt catggcgtca tcgaacgctt gtgcggggtg ggagtgacac tggaagaatc
      601 cgagcacgcg gaccacttgc gcgtgatctg ccactcgatg tgatgtcctc tcgggaatcc
      661 ttctgttggt ccgcttaatc aatgcaggcg cgtgcgggag ccccttcttc accgcatcca
      721 aggcgggtcg tccgccgagg caggccagcg ctacaagatg atcattagtg agtgcggcca
      781 gcgcggggtc cggcctcgaa agctgggcca cgattgactc cagtgcgggt ctgcccccga
      841 tattggatgc aat
//
