LOCUS       sequence-446788-        4795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE1419m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in striatal Pvalb+/Pthlh+ neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Hunker AC, Wirthlin ME, Gill G, Johansen NJ, Hooper M, Omstead V, 
            Vargas S, Lerma MN, Taskin N, Weed N, Laird WD, Bishaw YM, Bendrick 
            JL, Gore BB, Ben-Simon Y, Opitz-Araya X, Martinez RA, Way SW, 
            Thyagarajan B, Otto S, Sanchez REA, Alexander JR, Amaya A, Amster A,
            Arbuckle J, Ayala A, Baker PM, Barcelli T, Barta S, Bertagnolli D, 
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chong P, Clark M, Colbert K, 
            Daniel S, Dawe T, Departee M, DiValentin P, Donadio NP, Dotson NI, 
            Dwivedi D, Egdorf T, Fliss T, Gary A, Goldy J, Grasso C, Groce EL, 
            Gudsnuk K, Han W, Haradon Z, Hastings S, Helback O, Ho WV, Huang C, 
            Johnson T, Jones DL, Juneau Z, Kenney J, Leibly M, Li S, Liang E, 
            Loeffler H, Lusk NA, Madigan Z, Malloy J, Malone J, McCue R, Melchor
            J, Mich JK, Moosman S, Morin E, Naidoo R, Newman D, Ngo K, Nguyen K,
            Oster AL, Ouellette B, Oyama AA, Pena N, Pham T, Phillips E, Pom C, 
            Potekhina L, Ransford S, Ray PL, Reding M, Rette DF, Reynoldson C, 
            Rimorin C, Sigler AR, Rocha DB, Ronellenfitch K, Ruiz A, Sawyer L, 
            Sevigny JP, Shapovalova NV, Shepard N, Shulga L, Soliman S, Staats 
            B, Taormina MJ, Tieu M, Wang Y, Wilkes J, Wood T, Zhou T, Williford 
            A, Dee N, Mollenkopf T, Ng L, Esposito L, Kalmbach BE, Yao S, Ariza 
            J, Collman F, Mufti S, Smith K, Waters J, Ersing I, Patrick M, Zeng 
            H, Lein ES, Kojima Y, Horwitz G, Owen SF, Levi BP, Daigle TL, Tasic 
            B, Bakken TE, Ting JT
  TITLE     Enhancer AAV toolbox for accessing and perturbing striatal cell 
            types and circuits.
  JOURNAL   Neuron. 2025 May 21;113(10):1507-1524.e17. doi: 
            10.1016/j.neuron.2025.04.035.
  PUBMED    40403704
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.04"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1507-1524.e17"
COMMENT     Sequence Label: AiP15508 - pAAV-AiE1419m-minBG-iCre(R297T)-BGH...
FEATURES             Location/Qualifiers
     source          1..4795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      731..740
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             743..763
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1829..1940
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2058..2198
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2058..2187
                     /label=AAV2 ITR
     rep_origin      2273..2728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2360..2379)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2570..2591
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2745..2764)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2864..2886
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2924..2942)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3010..3114
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3115..3183,3184..3975)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3333..3352)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4146..4734
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4635..4654
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacttg acagtggtgg gcaaaagtaa aacttgaaag
      181 acaaaacttg gaagtgctgg aactgtaact ccagaggtct ctgagagagc ctggcttcga
      241 gaactcttta actccctctt ctcagcctct gagcagcaaa cccagcacag ctgttctcgc
      301 tgtaaagggc agctatgcag ggcagagttc ccagaaggct tctatgctct caaatccgct
      361 ctataaagta ggaagtgtgc tgagtaccag gccatccata ctgacaacca cagcagatcc
      421 accgccaagc atttttattg ttgcggcaga tggtggcctg attgtgaagc gtcccagagc
      481 aagggtgccc ttcaacattg gaaattaata gctaagctac tcatctccct tcaaatcaga
      541 ggcaatgtca ggctccgaga caaccttcag ggtagtttaa ctgagtccgg aagctgccag
      601 agcaggaccc atgtgggcca gccactgtcc ctctttctgt agctgactgg agctcgggct
      661 gggcataaaa gtcagggcag agccatctat tgcttacatt tgcttctggg atccagatct
      721 ttcgaagcta gccaccatgg tgcccaagaa gaagaggaaa gtctccaacc tgctgactgt
      781 gcaccaaaac ctgcctgccc tccctgtgga tgccacctct gatgaagtca ggaagaacct
      841 gatggacatg ttcagggaca ggcaggcctt ctctgaacac acctggaaga tgctcctgtc
      901 tgtgtgcaga tcctgggctg cctggtgcaa gctgaacaac aggaaatggt tccctgctga
      961 acctgaggat gtgagggact acctcctgta cctgcaagcc agaggcctgg ctgtgaagac
     1021 catccaacag cacctgggcc agctcaacat gctgcacagg agatctggcc tgcctcgccc
     1081 ttctgactcc aatgctgtgt ccctggtgat gaggagaatc agaaaggaga atgtggatgc
     1141 tggggagaga gccaagcagg ccctggcctt tgaacgcact gactttgacc aagtcagatc
     1201 cctgatggag aactctgaca gatgccagga catcaggaac ctggccttcc tgggcattgc
     1261 ctacaacacc ctgctgcgca ttgccgaaat tgccagaatc agagtgaagg acatctcccg
     1321 caccgatggt gggagaatgc tgatccacat tggcaggacc aagaccctgg tgtccacagc
     1381 tggtgtggag aaggccctgt ccctgggggt taccaagctg gtggagagat ggatctctgt
     1441 gtctggtgtg gctgatgacc ccaacaacta cctgttctgc cgggtcagaa agaatggtgt
     1501 ggctgcccct tctgccacct cccaactgtc cacccgggcc ctggaaggga tctttgaggc
     1561 cacccaccgc ctgatctatg gtgccaagga tgactctggg cagagatacc tggcctggtc
     1621 tggccactct gccagagtgg gtgctgccac cgacatggcc agggctggtg tgtccatccc
     1681 tgaaatcatg caggctggtg gctggaccaa tgtgaacatt gtgatgaact acatcagaaa
     1741 cctggactct gagactgggg ccatggtgag gctgctcgaa gatggggact gaggcgcgcc
     1801 gaattcaagc ttctcgagag atcttcgact gtgccttcta gttgccagcc atctgttgtt
     1861 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1921 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1981 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatga gatctcacgt
     2041 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     2101 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     2161 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     2221 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     2281 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2341 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2401 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     2461 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     2521 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     2581 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
     2641 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     2701 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     2761 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     2821 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     2881 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     2941 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     3001 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3061 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3121 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3181 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3241 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3301 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     3361 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3421 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3481 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     3541 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3601 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3661 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3721 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3781 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3841 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3901 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3961 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4021 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4081 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4141 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4201 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4261 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     4321 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4381 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4441 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4501 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4561 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4621 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4681 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4741 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
