LOCUS       sequence-446815-        9104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac vector encoding doxycycline-inducible codon-optimized human
            ETV2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Ding Y, Tamhankar S, Du F, Christopherson T, Schlueter N, Cohen JR, 
            Shusta EV, Palecek SP
  TITLE     ETV2 Overexpression Promotes Efficient Differentiation of 
            Pluripotent Stem Cells to Endothelial Cells.
  JOURNAL   Biotechnol Bioeng. 2025 Jul;122(7):1914-1928. doi: 
            10.1002/bit.28979. Epub 2025 Mar 25.
  PUBMED    40134129
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.28979";
            journalName: "Biotechnol Bioeng"; date: "2025-07"; volume: "122"; 
            issue: "7"; pages: "1914-1928"
COMMENT     Sequence Label: PB-TRE-ETV2
FEATURES             Location/Qualifiers
     source          1..9104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1385..1763
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1393..1411
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1429..1447
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1465..1483
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1501..1519
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1537..1555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1573..1591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1609..1627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1672..1696
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1790..1799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2876..2895)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2920..2968
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        3199..3410
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3423..3691
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3725..4468
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             4469..4522
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4544..5563
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             4544..5563
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(5620..5640)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5674..5795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5711..5730)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5765..5784
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6558..6592
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7083..7099)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7083..7099)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7096..7118)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7107..7123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7131..7137,7138..7155,7156..7161))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7176..7197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7314..7331)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7485..8073)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7565..7584)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8244..9035,9036..9104))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8867..8886
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtctttcgtc ttcaagaatt
     1381 cctcgagttt actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg
     1441 atagagaacg tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac
     1501 tccctatcag tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta
     1561 tctacagttt actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg
     1621 atagagaacg tataagcttt aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt
     1681 agtgaaccgt cagatcgcct ggagcaattc cacaacactt ttgtcttata ccaactttcc
     1741 gtaccacttc ctaccctcgt aaaggtctag agctagcgaa ttcgaatttg ccaccatgga
     1801 cctttggaac tgggatgaag caagtccgca agaagtacca cctgggaaca agctggcggg
     1861 gctggaagga gccaaactcg ggttttgctt cccggatctt gcactccagg gtgatactcc
     1921 tactgcaaca gccgaaacat gttggaaagg aacatcttct tcactcgcct cattccctca
     1981 gctggattgg ggatcagcac tgctgcatcc agaagtccca tggggcgcag agcctgactc
     2041 tcaggccctg ccgtggtccg gagactggac agacatggct tgcaccgcct gggactcttg
     2101 gtccggtgca tcccaaacgc ttggtccagc gccactcggt ccgggtccca ttccagctgc
     2161 tggaagtgaa ggggccgcag gccagaactg tgttcctgta gcgggcgaag ctacgtcctg
     2221 gtcccgggca caagcagccg gttctaatac ctcatgggat tgtagcgttg gccccgacgg
     2281 cgatacgtac tggggctccg ggctgggggg cgaacctcgg accgactgta caatcagttg
     2341 gggtgggcca gctggcccag attgtactac ttcctggaat cccggattgc atgcgggggg
     2401 gactacttca ctcaagaggt atcaaagttc cgccctgaca gtgtgcagcg agccctctcc
     2461 tcaatcagac cgagcgtctt tggctcggtg cccgaagact aaccacaggg gtcccataca
     2521 actgtggcaa tttttgctgg aattgctgca cgacggtgct agaagtagtt gcatacggtg
     2581 gactggaaac tctagggaat ttcagttgtg tgaccccaag gaagtcgcac gcctttgggg
     2641 ggaacgaaag agaaagcccg gcatgaacta tgagaagttg agtagaggtc ttcggtacta
     2701 ttatcgaaga gacattgtgc ggaaatcagg aggcaggaag tatacatata gatttggcgg
     2761 acgagtgcca agccttgcgt atcctgactg tgcgggaggg gggcgaggtg cggaaactca
     2821 gtaaaagggt tcgatcccta ccggttagta atgagtttaa acgggggagg ctaactgaaa
     2881 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     2941 aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     3001 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     3061 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     3121 ggccctgcca tagcagatct gcgaaatcgg atccagctaa atctaagtgg atctgcgatc
     3181 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     3241 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     3301 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     3361 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc
     3421 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc
     3481 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct
     3541 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac
     3601 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg
     3661 tttcgttttc tgttctgcgc cgttacagat ctaagctgtg accggcgcct actctagagc
     3721 cgccatgtct agactggaca agagcaaagt cataaactct gctctggaat tactcaatgg
     3781 agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     3841 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     3901 gctggacagg catcataccc actcctgccc cctggaaggc gagtcatggc aagactttct
     3961 gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt
     4021 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     4081 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     4141 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     4201 gacacctacc accgattcta tgcccccact tctgaaacaa gcaattgagc tgttcgaccg
     4261 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     4321 acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat
     4381 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     4441 cgattttgac cttgacatgc tccccgggga gggcagagga agtcttctaa catgcggtga
     4501 cgtggaggag aatcccggcc ctgaattcat ggatagatcc ggaaagcctg aactcaccgc
     4561 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     4621 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     4681 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     4741 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
     4801 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     4861 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
     4921 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     4981 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     5041 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     5101 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     5161 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     5221 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     5281 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     5341 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     5401 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     5461 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     5521 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa taagccaagg aacctctgga
     5581 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5641 tggatacgct gctttaatgc ctttgtatca gttaacttgt ttattgcagc ttataatggt
     5701 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5761 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggaattg actcaaatga
     5821 tgtcaattag tctatcagaa gctcatctgg tctcccttcc gggggacaag acatccctgt
     5881 ttaatattta aacagcagtg ttcccaaact gggttcttat atcccttgct ctggtcaacc
     5941 aggttgcagg gtttcctgtc ctcacaggaa cgaagtccct aaagaaacag tggcagccag
     6001 gtttagcccc ggaattgact ggattccttt tttagggccc attggtatgg ctttttcccc
     6061 gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat
     6121 cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg
     6181 gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg
     6241 gacgtaatta catccctggg ggctttgggg gggggctgtc cctgatatct ataacaagaa
     6301 aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta
     6361 tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc
     6421 gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca
     6481 agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga
     6541 gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaatcta
     6601 gctgcatcag gatcatatcg tcgggtcttt tttccggctc agtcatcgcc caagctggcg
     6661 ctatctgggc atcggggagg aagaagcccg tgccttttcc cgcgaggttg aagcggcatg
     6721 gaaagagttt gccgaggatg actgctgctg cattgacgtt gagcgaaaac gcacgtttac
     6781 catgatgatt cgggaaggtg tggccatgca cgcctttaac ggtgaactgt tcgttcaggc
     6841 cacctgggat accagttcgt cgcggctttt ccggacacag ttccggatgg tcagcccgaa
     6901 gcgcatcagc aacccgaaca ataccggcga cagccggaac tgccgtgccg gtgtgcagat
     6961 taatgacagc ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc ctggctggat
     7021 gccgcagaaa tggacatgga taccccgtga gttacccggc gggcgcgctt ggcgtaatca
     7081 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7141 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7201 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7261 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7321 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7381 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7441 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7501 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7561 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7621 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7681 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7741 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7801 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7861 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7921 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7981 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8041 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8101 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8161 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8221 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8281 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8341 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8401 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8461 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8521 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8581 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8641 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8701 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8761 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8821 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8881 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8941 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9001 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9061 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcat
//
