LOCUS       sequence-446835-       13190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human stromal interaction 
            molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming 
            virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13190)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..13190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2414..2423
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2423..2452
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4141..4158
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4153..4179
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHYHHHHHH"
     primer_bind     complement(4942..4962)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5360..5371)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6410..6429
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      6603..6612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6609..6611,6612..6614,6615..7328)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6654..6675)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6915..6934)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7262..7283
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             7423..7656
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7681..7699)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7682..7699)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7989..8008)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8222..8241)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8341..8363
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8401..8419)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8487..8591
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8592..8660,8661..9452)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8810..8829)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9623..10211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10112..10131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10365..10382
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10452..10472)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10457..10786
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10637..10772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11920..11985
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12115..12135
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12560..12694
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12597..12616)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12651..12670
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(12862..13190,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccaccg gtcgccacca tggaacaaaa actcatctca gaagaggatc tgatggatgt
     2461 atgcgtccgt cttgccctgt ggctcctctg gggactcctc ctgcaccagg gccagagcct
     2521 cagccatagt cacagtgaga aggcgacagg aaccagctcg ggggccaact ctgaggagtc
     2581 cactgcagca gagttttgcc gaattgacaa gcccctgtgt cacagtgagg atgagaaact
     2641 cagcttcgag gcagtccgta acatccacaa actgatggac gatgatgcca atggtgatgt
     2701 ggatgtggaa gaaagtgatg agttcctgag ggaagacctc aattaccatg acccaacagt
     2761 gaaacacagc accttccatg gtgaggataa gctcatcagc gtggaggacc tgtggaaggc
     2821 atggaagtca tcagaagtat acaattggac cgtggatgag gtggtacagt ggctgatcac
     2881 atatgtggag ctgcctcagt atgaggagac cttccggaag ctgcagctca gtggccatgc
     2941 catgccaagg ctggctgtca ccaacaccac catgacaggg actgtgctga agatgacaga
     3001 ccggagtcat cggcagaagc tgcagctgaa ggctctggat acagtgctct ttgggcctcc
     3061 tctcttgact cgccataatc acctcaagga cttcatgctg gtggtgtcta tcgttattgg
     3121 tgtgggcggc tgctggtttg cctatatcca gaaccgttac tccaaggagc acatgaagaa
     3181 gatgatgaag gacttggagg ggttacaccg agctgagcag agtctgcatg accttcagga
     3241 aaggctgcac aaggcccagg aggagcaccg cacagtggag gtggagaagg tccatctgga
     3301 aaagaagctg cgcgatgaga tcaaccttgc taagcaggaa gcccagcggc tgaaggagct
     3361 gcgggagggt actgagaatg agcggagccg ccaaaaatat gctgaggagg agttggagca
     3421 ggttcgggag gccttgagga aagcagagaa ggagctagaa tctcacagct catggtatgc
     3481 tccagaggcc cttcagaagt ggctgcagct gacacatgag gtggaggtgc aatattacaa
     3541 catcaagaag caaaatgctg agaagcagct gctggtggcc aaggaggggg ctgagaagat
     3601 aaaaaagaag agaaacacac tctttggcac cttccacgtg gcccacagct cttccctgga
     3661 tgatgtagat cataaaattc taacagctaa gcaagcactg agcgaggtga cagcagcatt
     3721 gcgggagcgc ctgcaccgct ggcaacagat cgagatcctc tgtggcttcc agattgtcaa
     3781 caaccctggc atccactcac tggtggctgc cctcaacata gaccccagct ggatgggcag
     3841 tacacgcccc aaccctgctc acttcatcat gactgacgac gtggatgaca tggatgagga
     3901 gattgtgtct cccttgtcca tgcagtcccc tagcctgcag agcagtgttc ggcagcgcct
     3961 gacggagcca cagcatggcc tgggatctca gaggttggta gagggcgagg ctggccactt
     4021 cttgacaagc cgggtatctc tgcggcgaat gcgcagcctt tcatctggac agtctttcag
     4081 ttctgaaggc tacgggacca gctctccatc tgcctctgct gctgcttctt gctcctcttc
     4141 catcaccacc atcaccacta ccaccaccac caccaccacc ttcaccaccg tccatgtcca
     4201 ccctgtttat taccaccaca gcacttccta tttcctccag atggagccct accctgacac
     4261 acccccttct gacagcaccg ctgtgatgcc tgggcattca gagagcttgg gggatttgac
     4321 ccattccgat tcggagtcct ccctccacat gagtgaccgc cagcgtgtgg cccccaaacc
     4381 tcctcagatg agccgtgctg cagacgaggc tctcaatgcc atgacttcca atggcagcca
     4441 ccggctgatc gagggggtcc acccagggtc tctggtggag aaactgcctg acagccctgc
     4501 cctggccaag aaggcattac tggcgctgaa ccatgggctg gacaaggccc acagcctgat
     4561 ggagctgagc ccctcagccc cacctggtgg ctctccacat ttggattctt cccgttctca
     4621 cagccccagc tccccagacc cagacacacc atctccagtt ggggacagcc gagccctgca
     4681 agccagccga aacacacgca ttccccacct ggctggcaag aaggctgtgg ctgaggagga
     4741 taatggctct attggcgagg aaacagactc cagcccaggc cggaagaagt ttcccctcaa
     4801 aatctttaag aagcctctta agaagtagag cggccgcgac tctagagtcg acctgcaggc
     4861 atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     4921 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4981 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5041 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5101 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5161 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     5221 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     5281 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5341 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcgggcgccc
     5401 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
     5461 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
     5521 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
     5581 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
     5641 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
     5701 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
     5761 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
     5821 ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
     5881 aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga
     5941 cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag
     6001 aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct
     6061 ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc
     6121 gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg
     6181 agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg
     6241 ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc
     6301 ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg
     6361 aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg
     6421 ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt
     6481 gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct
     6541 aaattctggc cgtttttggc ttttttgtta gacgaagctt ggtaccgagc tcggatcaat
     6601 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     6661 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     6721 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     6781 ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc taccccgacc
     6841 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     6901 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     6961 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     7021 tggggcacaa gctggagtac aactacaaca gccacaaggt ctatatcacc gccgacaagc
     7081 agaagaacgg catcaaggtg aacttcaaga cccgccacaa catcgaggac ggcagcgtgc
     7141 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     7201 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     7261 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     7321 acaagtaact cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg
     7381 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     7441 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     7501 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7561 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7621 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat
     7681 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
     7741 gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac
     7801 cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt
     7861 ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc
     7921 tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     7981 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     8041 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     8101 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     8161 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca
     8221 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     8281 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     8341 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     8401 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     8461 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8521 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8581 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8641 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8701 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8761 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8821 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8881 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8941 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     9001 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     9061 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     9121 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     9181 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     9241 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     9301 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     9361 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     9421 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     9481 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     9541 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9601 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9661 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9721 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9781 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9841 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9901 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9961 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10021 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    10081 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    10141 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    10201 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    10261 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    10321 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    10381 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    10441 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
    10501 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
    10561 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
    10621 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
    10681 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    10741 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
    10801 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
    10861 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
    10921 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
    10981 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
    11041 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
    11101 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
    11161 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
    11221 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
    11281 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
    11341 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
    11401 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
    11461 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
    11521 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
    11581 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
    11641 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
    11701 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
    11761 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
    11821 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
    11881 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
    11941 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
    12001 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
    12061 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
    12121 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
    12181 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
    12241 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
    12301 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
    12361 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
    12421 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
    12481 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    12541 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    12601 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    12661 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
    12721 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
    12781 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
    12841 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
    12901 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
    12961 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
    13021 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
    13081 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
    13141 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
//
