LOCUS       sequence-446840-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike G446S for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 G446S (AGC)
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      518..1106
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1007..1026
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    1302..1526
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(1537..2331)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1648..1667)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2258..2277
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2345..2541)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2359..2494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    2586..2657
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(2702..2838)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(2868..2914)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3029..3150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3066..3085)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3120..3139
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    3203..3791
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3309..3320
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3718..3738
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     intron          7688..8163
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     promoter        complement(8297..8499)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8326..8346)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8501..8804
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    8885..8933
                     /note="synthetic polyadenylation signal"
     terminator      8951..8997
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    9002..9073
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9225..9361)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(9374..10165,10166..10234))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9997..10016
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatcgttctc ggtacaccag gcgcgccccg atttcgatat tcctcggatc tcggatagat
       61 cgggattgag gatgtaggtt gaacttctct cgggtgatcc cgatctcgga tattcgggat
      121 tggagtaata cctggcgata ggcgctgcca agcaatagcc aaggcgcaag gccggccaag
      181 agacttgcga tagttcgttc gagaggtagc ctgcaagatg gatcggacga aaggtttgaa
      241 gattttccga cgagggcctt ggagagttcg gaaatttaaa tgcaactatg gatgaacgaa
      301 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
      361 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
      421 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      481 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      541 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      601 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      661 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      721 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      781 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      841 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      901 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      961 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1021 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1081 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1141 ccttttgctg gccttttgct cacatgattt aaatgaaact cgtaatcgga gaacgggtgc
     1201 aatagcaagg accagatcga acttgactga cgcaattctg agcgtagtcg aaacccagac
     1261 aagtcgatct cgccaagtag ctgacttgac tggttaatta accatagagc ccaccgcatc
     1321 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
     1381 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
     1441 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
     1501 agatggctgg caactagaag gcacagggct tacatactag aagaactcgt caagaaggcg
     1561 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     1621 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     1681 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     1741 catgatattc ggcaagcagg catcgccgtg ggtcacgacg agatcctcgc cgtcgggcat
     1801 ccgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     1861 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     1921 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     1981 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     2041 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     2101 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct ggagttcatt
     2161 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     2221 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     2281 ctccacccaa gcggcgggag aacctgcgtg caatccatct tgttcaatca tgttggctac
     2341 ctcctttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
     2401 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcga tggggcggag
     2461 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
     2521 ttgctgacta attgagatgc agatcgctta attaagtttc gatagcccaa ggtaaccaat
     2581 gagattaggg agggaagaaa gtttgatgaa ctacttctga cccctagtgg tgtccagaaa
     2641 agaccattaa aggaatgtct tgatcgaaat cgagagatcg cctgcaggat agctgacgac
     2701 cttaaggata aatttctggt aaggaggaca cgtatggaag tgggcaagtt ggggaagccg
     2761 tatccgttgc tgaatctggc atatgtggga gtataagacg cgcagcgtcg catcaggcat
     2821 ttttttctgc gccaatgccg gtagatagca atccggcccg aggggcacaa aaaacccctc
     2881 aagacccgtt tagaggcccc aaggggttat gctacgatac cgatagagat gggccaatac
     2941 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     3001 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     3061 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3121 tggtttgtcc aaactcatca atgtatctta tcatgtctgt gagctgaagg tacgctgtat
     3181 ctcagtcagt caagctagac aggcggggag gcggcccaaa gggagatccg actcgtctga
     3241 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     3301 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     3361 gcaacacagg cgagcagcca tggaaaggac gtcagcttcc ccgacaacac cacggaattg
     3421 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     3481 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     3541 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     3601 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     3661 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     3721 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     3781 agaggttgat taggcctgcg gatccagaaa tcgattgtat cagtcagtca gtgcaggagg
     3841 agacaacttc tagaggatcc tttatttaca acaagatccg caactgcaac atcctttcaa
     3901 gcaggaacaa cagctagtca tacagcacaa cataattgtg accataacga tagctataag
     3961 tccggcgata aagcccaacc aaatgtacca gggccattta atatattgtt cgtatttgcc
     4021 cagttcttgc aagtcgatca aagattcatt caagttcttg gcgacctcat taaggcgatc
     4081 aatctctttc tgaatgttga cgacacttgc atttattcct gatatgtccc cgagatcaac
     4141 atcgggtgaa gtatgattct taaagtattt atccaattcc tctttgaagc tatccaactc
     4201 gggttgcagg ggatcatata ccgtgttgtt cacgatccca atgaccacat cacagttgcc
     4261 tgagacaaat gtattgtcag tggttatgat ttggggttca tagaaattcc tttgggtcac
     4321 aaaccaatgc gtgccattac tgacgaaaac gccttccctg ggaaagtgcg ctttgccatc
     4381 gtgacagatt gctggagctg tcgtaaagtt cttctcttga gcagggacat aagtgacgtg
     4441 gagaaagacc actccatgtg gggcggattg ggggaaggac atcaagtgat agcccttgcc
     4501 acaaaagtca acccgtttgg actgcccgag cacgcactct gacattttag tggcggccag
     4561 gttggcgctt gctcgtatct cagcagcccg gatcaactgt tgtgtcacat aagtttggag
     4621 tgactggagc ctgcctgtga tgagccgatc aatttgcacc tcggcttcca ccttatcaag
     4681 tctggacaat atgtcgttga ggacgctgga gattgcgccg aagttagaac tcagttgctt
     4741 cacgagggta ttcagggcct gggcgttttg attcacaaca tcttggagtt tgccgagggc
     4801 ggacgctgtg cttgacagtg aatcttggat cttgccaatc gcgctgttaa attgattagc
     4861 gatcagcttc tgattttcat acaatacatt ttgcgtgaca ccaatcccat tgaatctgta
     4921 agccatttgc atagcgaaag gtatttgaag ggccgcaccc gcgccaaaag tccagccgga
     4981 tgtaatggtt cctgccaaca atgctgatgt atactgtgca atcatctcat cagtgagcag
     5041 agggggcaga actgtcaacc cattgaattt ttgagcacag ataaggtcgc gggccgcaat
     5101 gtctcccaaa caatccccat actgcttaat gaaacccgcg tcagccaacg taactttgtt
     5161 gaacagcaga tcctcgataa agctgcgttt tgaaggctta gatgggtctg gcagtatttg
     5221 gctaaaatta aacccgccaa aatctttgat tggcggcgtt ttgtatattt gctttacttg
     5281 tgcgaacacc tcttgggtat tcttgtcttg ttcgaccgca attccggtca atgctcggtt
     5341 gagttgagta cagaaagacc cgtattgaag caagaggttg ctacactcgg tggaatcccc
     5401 acagatatac atcgtacagt ctacgctcgt cttagtcatg ctgacgggca agatttccgt
     5461 ggtaacgcta atggtaaagt tagttgggat tgcgatggaa ttgtttgaat aagccacgga
     5521 gttctcagcc ccgagactca tggtataggc gattatggac tggctggcta ctgagcgagc
     5581 gcgccgtggt gagttcgttt gagtttgata agacgcacag attcctgccc caataggaat
     5641 atcacattcg tatgaattat tgacatgctc agcgccaatg agacaacccg ctctagtttg
     5701 aaagacattg gaacctgttg agtagactct ccaggtgggt gtcagttggt cggcgtgtat
     5761 cgccacaggg acttcggtac agttgacatc ttgatacagg acagccactt ggttggatgt
     5821 gttcgttccg ggagttatca cgctgacccc gccgaagcta cagggtgtta tatcgaggat
     5881 ttccagtgtt tgcggatcgc ggacggcatc tgtggtatcg gcaatgtctc taccaaattg
     5941 ttgaaaaggg agaaatttct tgtttgattc tgtcagcacc cctgttccgg tgagcccatt
     6001 aaagttgaag ttcacacatt tattcttcac caggttagtg cttttcttcg gtccgcaaac
     6061 tgtagccggt gcgtggagca gctcaaagct caagactacc acccgatatg gttgatagcc
     6121 caccccattt gttggttgaa atccgtatga ttgaagggga aaataacagt taaacccttc
     6181 cactccatta catggggtgc tgccagcttg ataaatttcg gtggagatgt cacgttcaaa
     6241 gggcttcaga tttgacttgc gaaacaggcg gtaaagataa ttatagttgc cgctgacttt
     6301 tgaatcgaga ttattggagt tccatgctat aacgcagccg gtgaagtcat ccggcaactt
     6361 ataattatag tctgctatct ttccggtttg ccccggtgca atctgtcgga cttcgtcgcc
     6421 gcgtatgacg aaagaatctg cgtaaacgtt ggtaaaacac aagtcattca atttcgtagg
     6481 gctgacccca taacacttaa aagtgctgaa tgatgcgcta ttatacagga cactataatc
     6541 tgcaacacaa tttgatattc gcttgcgatt ccaggcataa actgatgcaa atcgagtggc
     6601 attaaatact tcaccgaaag gacacaaatt ggtgatgtta gggaatcgaa caatggattc
     6661 ggtgggttgg acgcggaagt tactggtttg gtatatgcct ttctcaactg tgaaggactt
     6721 gagtgtgcat ttagtttcgg acaggggatc gagggcacag tctacggcat cggtaattgt
     6781 tccgttttcg ttatatttga gaagaaaggt gcgtggttgc aaatatccca catagtaggc
     6841 tgctgcgccc gctgtccaac cactactact gtcgccaggc gtgaggtagc ttcgatgaag
     6901 ggcaagaagg gtttggaacc gggtgatgtt gatgccaatc ggcaagtcca cgaggggttc
     6961 caacgcggag aagccttgag ggagatcccg aaccagattt atcggggtat gcttgctata
     7021 aattttgaag tagccgtcta tattcttaaa cacaaattcg cggagattct tgaagttgcc
     7081 ttgcttccct tcaagatcca tgagaaatgg ttgggaaacg tactcaaaag tacagttgtt
     7141 agcggaactg taaacgcgga actctgactc catccaagac ttgttattct tatgataata
     7201 aaccccgaga aagggatcgt tacaaaattg aaattcacag actttgatga cgacattggt
     7261 ggcgttgttc acaatgagga gagactgtgt cttggagtcc agggtggtgc caaatatcca
     7321 tccccggata atgttggact tctccgttga ggcaaagtat acgccatcgt taaatggcaa
     7381 gacgggatta tcaaatcgtt tagttccgtt ggtgccagaa acgtggatag catggaacca
     7441 ggtgacattt gagaagaatg gcaggaaaag gtcttgagta gaatgaagga cactggacct
     7501 aaagacttta tcgggatagt atactccacg agtaaaagag ttcgtgtaag cgggtgggag
     7561 ctgcgttcta gttgtgaggt tcacgcattg ggaagaaacg aggggcagga gaacgaggaa
     7621 cacaaacatg gcggccgcct ttgccaaagt gatgggccag cacacagacc agcacgttgc
     7681 ccaggagctg tgggaggaag ataagaggta tgaacatgat tagcaaaagg gcctagcttg
     7741 gactcagaat aatccagcct tatcccaacc ataaaataaa agcagaatgg tagctggatt
     7801 gtagctgcta ttagcaatat gaaacctctt acatcagtta caatttatat gcagaaatat
     7861 ttatatgcag aaatattgct attgccttaa cccagaaatt atcactgtta ttctttagaa
     7921 tggtgcaaag aggcatgata cattgtatca ttattgccct gaaagaaaga gattagggaa
     7981 agtattagaa ataagataaa caaaaaagta tattaaaaga agaaagcatt ttttaaaatt
     8041 acaaatgcaa aattaccctg atttggtcaa tatgtgtacc ctgttacttc tccccttcct
     8101 atgacatgaa cttaaccata gaaaagaagg ggaaagaaaa catcaagggt cccatagact
     8161 caccctgaag ttctcaggat ccgagctcgg taccacatgt aagcttcgag gggaggctgg
     8221 atcggtcccg gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc caggcgatct
     8281 gacggttcac taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
     8341 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
     8401 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
     8461 gctatccacg cccattgatg tactgccaaa accgcatcag catggtaata gcgatgacta
     8521 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     8581 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     8641 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     8701 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
     8761 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
     8821 ggctatgaac taatgacccc gtaattgatt actagatctg gctacactcg atcagcagtt
     8881 agataataaa atcgctatcc atcgaagatg gatgtgtgtt ggttttttgt gtgtgtaacg
     8941 caacgattga tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgaat
     9001 tgagaggtag ctgaagctgc tgttatgacc actagaggga agaagatacc tgtggagcta
     9061 atggtccaag atgtcgggag ccgcgatcgc tgccaaaaag gtaagctgac gatagctgac
     9121 gagattcgat gcccgacgag caaatgagcg ccacggctgt tcgcgtaagc tcgaaacgac
     9181 ggagattcga tagagccaga gtcgagttta aacgattcgg gatcttaagg ataaatttct
     9241 ggtaaggagg acacgtatgg aagtgggcaa gttggggaag ccgtatccgt tgctgaatct
     9301 ggcatatgtg ggagtataag acgcgcagcg tcgcatcagg catttttttc tgcgccaatg
     9361 cacctagaat cggttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9421 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9481 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9541 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9601 ccgcctccat ccagtctatc aactgttgcc gggaagctag agtaagtagt tcgccagtta
     9661 atagtttacg caaggttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9721 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9781 tgtgcaaaaa agcggttagc tccttcggtc ctccaatcgt tgtcagaagt aagttggccg
     9841 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9901 taagatgctt ttctgtgact ggtgaatact caaccaagtc attctgagaa tagtgtatgc
     9961 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10021 ctttaaaagt gctcatcatt ggaaagcgtt cttcggggcg aaaactctca aggatcttac
    10081 cgctgttgag atccagttcg atgtaaccca ctcgcgcacc caactgatct tcagcatctt
    10141 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10201 gaataagggc gacacggaaa tgttgaatac tcatgcgaaa gcctcctgct gatcctgtct
    10261 cttgatagat gtttgcaaaa gctaggactc gattatacgt tgaccgatca gactatcaat
    10321 atctaggttt aaacagatcg gtacgctgcc gtgaccaggc ttgagtacaa tatctgacga
    10381 ttggacttcc tgggtcccaa gctggattcc gactggctga ctgccaagca acgatatgga
    10441 tcgtcgagac tcgcactgag ag
//
