LOCUS       sequence-446843-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike G446S for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 G446S (AGC)
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    202..790
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(255..275)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(673..684)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    843..964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(854..873)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     908..927
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      1079..1125
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1155..1291
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    1336..1407
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        1452..1648
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1499..1634
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1662..2456
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1716..1735)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2326..2345
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2467..2691
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2887..3475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2967..2986)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(4221..4289,4290..5081)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4439..4458)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      5094..5230
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5382..5453
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(5458..5504)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    5522..5570
                     /note="synthetic polyadenylation signal"
     enhancer        5651..5954
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5956..6158
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6109..6129
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          6292..6767
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 cctggtacat ttggttgggc tttatcgccg gacttatagc tatcgttatg gtcacaatta
       61 tgttgtgctg tatgactagc tgttgttcct gcttgaaagg atgttgcagt tgcggatctt
      121 gttgtaaata aaggatcctc tagaagttgt ctcctcctgc actgactgac tgatacaatc
      181 gatttctgga tccgcaggcc taatcaacct ctggattaca aaatttgtga aagattgact
      241 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
      301 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
      361 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
      421 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
      481 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
      541 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
      601 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
      661 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
      721 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
      781 gcctccccgc ctgtctagct tgactgactg agatacagcg taccttcagc tcacagacat
      841 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
      901 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
      961 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     1021 tttttaaagc aagtaaaacc tctacaaatg tggtattggc ccatctctat cggtatcgta
     1081 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgtgccc ctcgggccgg
     1141 attgctatct accggcattg gcgcagaaaa aaatgcctga tgcgacgctg cgcgtcttat
     1201 actcccacat atgccagatt cagcaacgga tacggcttcc ccaacttgcc cacttccata
     1261 cgtgtcctcc ttaccagaaa tttatcctta aggtcgtcag ctatcctgca ggcgatctct
     1321 cgatttcgat caagacattc ctttaatggt cttttctgga caccactagg ggtcagaagt
     1381 agttcatcaa actttcttcc ctccctaatc tcattggtta ccttgggcta tcgaaactta
     1441 attaagcgat ctgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     1501 cccgccccta actccgccca gttccgccca ttctccgccc catcgctgac taattttttt
     1561 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     1621 cttttttgga ggcctaggct tttgcaaagg aggtagccaa catgattgaa caagatggat
     1681 tgcacgcagg ttctcccgcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     1741 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     1801 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact ccaggacgag gcagcgcggc
     1861 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     1921 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     1981 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     2041 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     2101 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     2161 cagccgaact gttcgccagg ctcaaggcgc ggatgcccga cggcgaggat ctcgtcgtga
     2221 cccacggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     2281 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     2341 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     2401 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctagtatg
     2461 taagccctgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     2521 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     2581 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     2641 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gttaattaac
     2701 cagtcaagtc agctacttgg cgagatcgac ttgtctgggt ttcgactacg ctcagaattg
     2761 cgtcagtcaa gttcgatctg gtccttgcta ttgcacccgt tctccgatta cgagtttcat
     2821 ttaaatcatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2881 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2941 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3001 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3061 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3121 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3181 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3241 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3301 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3361 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3421 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3481 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3541 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3601 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3661 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg catttaaatt
     3721 tccgaactct ccaaggccct cgtcggaaaa tcttcaaacc tttcgtccga tccatcttgc
     3781 aggctacctc tcgaacgaac tatcgcaagt ctcttggccg gccttgcgcc ttggctattg
     3841 cttggcagcg cctatcgcca ggtattactc caatcccgaa tatccgagat cgggatcacc
     3901 cgagagaagt tcaacctaca tcctcaatcc cgatctatcc gagatccgag gaatatcgaa
     3961 atcggggcgc gcctggtgta ccgagaacga tcctctcagt gcgagtctcg acgatccata
     4021 tcgttgcttg gcagtcagcc agtcggaatc cagcttggga cccaggaagt ccaatcgtca
     4081 gatattgtac tcaagcctgg tcacggcagc gtaccgatct gtttaaacct agatattgat
     4141 agtctgatcg gtcaacgtat aatcgagtcc tagcttttgc aaacatctat caagagacag
     4201 gatcagcagg aggctttcgc atgagtattc aacatttccg tgtcgccctt attccctttt
     4261 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4321 ctgaagatca gttgggtgcg cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4381 tccttgagag ttttcgcccc gaagaacgct ttccaatgat gagcactttt aaagttctgc
     4441 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4501 actattctca gaatgacttg gttgagtatt caccagtcac agaaaagcat cttacggatg
     4561 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4621 acttacttct gacaacgatt ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4681 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4741 acgagcgtga caccacgatg cctgtagcaa tggcaacaac cttgcgtaaa ctattaactg
     4801 gcgaactact tactctagct tcccggcaac agttgataga ctggatggag gcggataaag
     4861 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4921 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4981 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5041 agatcgctga gataggtgcc tcactgatta agcattggta accgattcta ggtgcattgg
     5101 cgcagaaaaa aatgcctgat gcgacgctgc gcgtcttata ctcccacata tgccagattc
     5161 agcaacggat acggcttccc caacttgccc acttccatac gtgtcctcct taccagaaat
     5221 ttatccttaa gatcccgaat cgtttaaact cgactctggc tctatcgaat ctccgtcgtt
     5281 tcgagcttac gcgaacagcc gtggcgctca tttgctcgtc gggcatcgaa tctcgtcagc
     5341 tatcgtcagc ttaccttttt ggcagcgatc gcggctcccg acatcttgga ccattagctc
     5401 cacaggtatc ttcttccctc tagtggtcat aacagcagct tcagctacct ctcaattcaa
     5461 aaaacccctc aagacccgtt tagaggcccc aaggggttat gctatcaatc gttgcgttac
     5521 acacacaaaa aaccaacaca catccatctt cgatggatag cgattttatt atctaactgc
     5581 tgatcgagtg tagccagatc tagtaatcaa ttacggggtc attagttcat agcccatata
     5641 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     5701 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     5761 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     5821 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     5881 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     5941 tcgctattac catgctgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     6001 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     6061 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     6121 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcg
     6181 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
     6241 tcccctcgaa gcttacatgt ggtaccgagc tcggatcctg agaacttcag ggtgagtcta
     6301 tgggaccctt gatgttttct ttccccttct tttctatggt taagttcatg tcataggaag
     6361 gggagaagta acagggtaca catattgacc aaatcagggt aattttgcat ttgtaatttt
     6421 aaaaaatgct ttcttctttt aatatacttt tttgtttatc ttatttctaa tactttccct
     6481 aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc accattctaa
     6541 agaataacag tgataatttc tgggttaagg caatagcaat atttctgcat ataaatattt
     6601 ctgcatataa attgtaactg atgtaagagg tttcatattg ctaatagcag ctacaatcca
     6661 gctaccattc tgcttttatt ttatggttgg gataaggctg gattattctg agtccaagct
     6721 aggccctttt gctaatcatg ttcatacctc ttatcttcct cccacagctc ctgggcaacg
     6781 tgctggtctg tgtgctggcc catcactttg gcaaaggcgg ccgccatgtt tgtgttcctc
     6841 gttctcctgc ccctcgtttc ttcccaatgc gtgaacctca caactagaac gcagctccca
     6901 cccgcttaca cgaactcttt tactcgtgga gtatactatc ccgataaagt ctttaggtcc
     6961 agtgtccttc attctactca agaccttttc ctgccattct tctcaaatgt cacctggttc
     7021 catgctatcc acgtttctgg caccaacgga actaaacgat ttgataatcc cgtcttgcca
     7081 tttaacgatg gcgtatactt tgcctcaacg gagaagtcca acattatccg gggatggata
     7141 tttggcacca ccctggactc caagacacag tctctcctca ttgtgaacaa cgccaccaat
     7201 gtcgtcatca aagtctgtga atttcaattt tgtaacgatc cctttctcgg ggtttattat
     7261 cataagaata acaagtcttg gatggagtca gagttccgcg tttacagttc cgctaacaac
     7321 tgtacttttg agtacgtttc ccaaccattt ctcatggatc ttgaagggaa gcaaggcaac
     7381 ttcaagaatc tccgcgaatt tgtgtttaag aatatagacg gctacttcaa aatttatagc
     7441 aagcataccc cgataaatct ggttcgggat ctccctcaag gcttctccgc gttggaaccc
     7501 ctcgtggact tgccgattgg catcaacatc acccggttcc aaacccttct tgcccttcat
     7561 cgaagctacc tcacgcctgg cgacagtagt agtggttgga cagcgggcgc agcagcctac
     7621 tatgtgggat atttgcaacc acgcaccttt cttctcaaat ataacgaaaa cggaacaatt
     7681 accgatgccg tagactgtgc cctcgatccc ctgtccgaaa ctaaatgcac actcaagtcc
     7741 ttcacagttg agaaaggcat ataccaaacc agtaacttcc gcgtccaacc caccgaatcc
     7801 attgttcgat tccctaacat caccaatttg tgtcctttcg gtgaagtatt taatgccact
     7861 cgatttgcat cagtttatgc ctggaatcgc aagcgaatat caaattgtgt tgcagattat
     7921 agtgtcctgt ataatagcgc atcattcagc acttttaagt gttatggggt cagccctacg
     7981 aaattgaatg acttgtgttt taccaacgtt tacgcagatt ctttcgtcat acgcggcgac
     8041 gaagtccgac agattgcacc ggggcaaacc ggaaagatag cagactataa ttataagttg
     8101 ccggatgact tcaccggctg cgttatagca tggaactcca ataatctcga ttcaaaagtc
     8161 agcggcaact ataattatct ttaccgcctg tttcgcaagt caaatctgaa gccctttgaa
     8221 cgtgacatct ccaccgaaat ttatcaagct ggcagcaccc catgtaatgg agtggaaggg
     8281 tttaactgtt attttcccct tcaatcatac ggatttcaac caacaaatgg ggtgggctat
     8341 caaccatatc gggtggtagt cttgagcttt gagctgctcc acgcaccggc tacagtttgc
     8401 ggaccgaaga aaagcactaa cctggtgaag aataaatgtg tgaacttcaa ctttaatggg
     8461 ctcaccggaa caggggtgct gacagaatca aacaagaaat ttctcccttt tcaacaattt
     8521 ggtagagaca ttgccgatac cacagatgcc gtccgcgatc cgcaaacact ggaaatcctc
     8581 gatataacac cctgtagctt cggcggggtc agcgtgataa ctcccggaac gaacacatcc
     8641 aaccaagtgg ctgtcctgta tcaagatgtc aactgtaccg aagtccctgt ggcgatacac
     8701 gccgaccaac tgacacccac ctggagagtc tactcaacag gttccaatgt ctttcaaact
     8761 agagcgggtt gtctcattgg cgctgagcat gtcaataatt catacgaatg tgatattcct
     8821 attggggcag gaatctgtgc gtcttatcaa actcaaacga actcaccacg gcgcgctcgc
     8881 tcagtagcca gccagtccat aatcgcctat accatgagtc tcggggctga gaactccgtg
     8941 gcttattcaa acaattccat cgcaatccca actaacttta ccattagcgt taccacggaa
     9001 atcttgcccg tcagcatgac taagacgagc gtagactgta cgatgtatat ctgtggggat
     9061 tccaccgagt gtagcaacct cttgcttcaa tacgggtctt tctgtactca actcaaccga
     9121 gcattgaccg gaattgcggt cgaacaagac aagaataccc aagaggtgtt cgcacaagta
     9181 aagcaaatat acaaaacgcc gccaatcaaa gattttggcg ggtttaattt tagccaaata
     9241 ctgccagacc catctaagcc ttcaaaacgc agctttatcg aggatctgct gttcaacaaa
     9301 gttacgttgg ctgacgcggg tttcattaag cagtatgggg attgtttggg agacattgcg
     9361 gcccgcgacc ttatctgtgc tcaaaaattc aatgggttga cagttctgcc ccctctgctc
     9421 actgatgaga tgattgcaca gtatacatca gcattgttgg caggaaccat tacatccggc
     9481 tggacttttg gcgcgggtgc ggcccttcaa atacctttcg ctatgcaaat ggcttacaga
     9541 ttcaatggga ttggtgtcac gcaaaatgta ttgtatgaaa atcagaagct gatcgctaat
     9601 caatttaaca gcgcgattgg caagatccaa gattcactgt caagcacagc gtccgccctc
     9661 ggcaaactcc aagatgttgt gaatcaaaac gcccaggccc tgaataccct cgtgaagcaa
     9721 ctgagttcta acttcggcgc aatctccagc gtcctcaacg acatattgtc cagacttgat
     9781 aaggtggaag ccgaggtgca aattgatcgg ctcatcacag gcaggctcca gtcactccaa
     9841 acttatgtga cacaacagtt gatccgggct gctgagatac gagcaagcgc caacctggcc
     9901 gccactaaaa tgtcagagtg cgtgctcggg cagtccaaac gggttgactt ttgtggcaag
     9961 ggctatcact tgatgtcctt cccccaatcc gccccacatg gagtggtctt tctccacgtc
    10021 acttatgtcc ctgctcaaga gaagaacttt acgacagctc cagcaatctg tcacgatggc
    10081 aaagcgcact ttcccaggga aggcgttttc gtcagtaatg gcacgcattg gtttgtgacc
    10141 caaaggaatt tctatgaacc ccaaatcata accactgaca atacatttgt ctcaggcaac
    10201 tgtgatgtgg tcattgggat cgtgaacaac acggtatatg atcccctgca acccgagttg
    10261 gatagcttca aagaggaatt ggataaatac tttaagaatc atacttcacc cgatgttgat
    10321 ctcggggaca tatcaggaat aaatgcaagt gtcgtcaaca ttcagaaaga gattgatcgc
    10381 cttaatgagg tcgccaagaa cttgaatgaa tctttgatcg acttgcaaga actgggcaaa
    10441 tacgaacaat atattaaatg gc
//
