LOCUS       sequence-446845-       12440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human atlastin GTPase 3 (ATL3) 
            fused to myc-tag under the spleen focus-forming virus (SFFV) 
            promoter and Emerald under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12440)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-myc-ATL3-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..12440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2414..2423
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2423..2452
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4192..4212)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4610..4621)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5660..5679
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5853..5862
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5859..5861,5862..5864,5865..6578)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5904..5925)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6165..6184)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6512..6533
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6673..6906
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6931..6949)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6932..6949)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7239..7258)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7472..7491)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7591..7613
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7651..7669)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7737..7841
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7842..7910,7911..8702)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8060..8079)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8873..9461
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9362..9381
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9615..9632
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9702..9722)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9707..10036
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9887..10022
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11170..11235
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11365..11385
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11810..11944
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11847..11866)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11901..11920
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(12112..12440,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccaccg gtcgccacca tggaacaaaa actcatctca gaagaggatc tgatgttgtc
     2461 ccctcagcga gtggcagcag ctgcctcaag aggagcagat gatgccatgg agagcagcaa
     2521 gcctggtcca gtgcaggttg ttttggttca gaaagatcaa cattcctttg agctagatga
     2581 gaaagccttg gccagcatcc tcttgcagga ccacatccga gatcttgatg tggtggtggt
     2641 ttcagtggct ggtgccttcc gaaagggcaa gtccttcatt ctggatttta tgctacgata
     2701 cttatattct cagaaggaaa gtggccattc aaattggttg ggtgacccag aagaaccgtt
     2761 aacaggattt tcctggagag ggggatctga tccagaaacc actgggattc aaatctggag
     2821 tgaagttttc actgtggaga agccaggtgg gaagaaggtt gcagttgttc tgatggatac
     2881 ccagggggca tttgacagcc agtcaactgt gaaagactgt gctaccatct ttgctctaag
     2941 cactatgact agttctgttc agatttataa tttatctcag aacattcaag aagatgatct
     3001 tcaacagctg cagctcttca cagaatacgg tcgtctggca atggatgaaa ttttccaaaa
     3061 gcctttccag acactgatgt ttttggttag agattggagt ttcccttatg aatatagcta
     3121 tggactccaa ggaggaatgg catttttgga taagcgttta caggtgaagg aacatcaaca
     3181 tgaagaaatt cagaatgttc gaaatcacat tcactcatgt ttctccgatg tcacctgctt
     3241 tctcttacca catccaggac tccaggtggc cacaagccct gactttgatg ggaaattaaa
     3301 agatattgct ggtgaattca aagagcagtt acaggcactg ataccgtatg tattaaaccc
     3361 atctaagtta atggaaaagg agatcaatgg ctcaaaggtc acctgtcggg gactactgga
     3421 gtattttaag gcatatatta aaatttatca aggagaagat ctgcctcacc ccaagtccat
     3481 gcttcaggcc actgctgaag ccaacaactt agcagctgca gcctctgcca aggacattta
     3541 ttataacaac atggaagagg tttgtggggg agagaaacct tatttgtctc cagacattct
     3601 agaggagaag cactgtgaat tcaaacaact tgctctggac cattttaaga agaccaagaa
     3661 gatgggtggg aaggatttca gctttcgtta ccagcaggag ctggaggagg aaatcaagga
     3721 attatatgag aacttctgca agcacaatgg tagcaagaac gtcttcagca ccttccgaac
     3781 ccctgcagtg ctgttcacgg gcattgtagc tttgtacata gcctcaggcc tcactggctt
     3841 cataggtctt gaggttgtag cccagttgtt caactgtatg gttggactac tgttaatagc
     3901 actcctcacc tggggctaca tcaggtattc tggtcaatat cgtgagctgg gcggagctat
     3961 tgattttggt gccgcatatg tgttggagca ggcttcttct catatcggta attccactca
     4021 ggccactgtg agggatgcag ttgttggaag accatccatg gataaaaaag ctcaatagag
     4081 cggccgcgac tctagagtcg acctgcaggc atgcaagctt gatatcaagc ttatcgataa
     4141 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4201 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4261 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4321 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4381 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4441 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4501 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4561 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4621 tccagcggac cttccttccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag
     4681 acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct
     4741 gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact
     4801 tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt
     4861 cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata
     4921 aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct
     4981 tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc
     5041 tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg
     5101 tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat
     5161 ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga
     5221 aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc
     5281 gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag
     5341 tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt
     5401 gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca
     5461 cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct
     5521 tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg
     5581 ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt
     5641 acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt
     5701 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
     5761 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
     5821 gacgaagctt ggtaccgagc tcggatcaat tcgccaccat ggtgagcaag ggcgaggagc
     5881 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     5941 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     6001 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttgacctacg
     6061 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     6121 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     6181 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     6241 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     6301 gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     6361 cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     6421 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     6481 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     6541 ccgggatcac tctcggcatg gacgagctgt acaagtaact cgagggaatt aattcgagct
     6601 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6661 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     6721 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6781 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6841 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6901 ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat
     6961 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa
     7021 ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag
     7081 agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat
     7141 ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt
     7201 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     7261 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     7321 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     7381 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     7441 taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     7501 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     7561 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     7621 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     7681 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     7741 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7801 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7861 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7921 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7981 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8041 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     8101 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8161 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8221 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8281 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8341 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8401 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8461 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8521 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8581 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8641 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8701 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8761 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8821 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8881 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8941 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9001 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9061 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9121 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9181 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     9241 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     9301 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9361 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     9421 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     9481 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     9541 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     9601 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     9661 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
     9721 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     9781 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     9841 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     9901 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     9961 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    10021 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
    10081 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
    10141 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
    10201 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
    10261 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
    10321 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
    10381 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
    10441 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
    10501 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
    10561 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
    10621 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
    10681 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
    10741 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
    10801 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
    10861 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
    10921 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
    10981 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
    11041 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
    11101 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
    11161 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
    11221 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
    11281 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
    11341 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
    11401 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
    11461 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
    11521 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
    11581 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
    11641 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
    11701 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
    11761 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    11821 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    11881 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    11941 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    12001 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    12061 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
    12121 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
    12181 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
    12241 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
    12301 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
    12361 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
    12421 cggagtactt caagaactgc
//
