LOCUS       sequence-446848-       10451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike Q52H for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 Q52H (CAT)
FEATURES             Location/Qualifiers
     source          1..10451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(234..436)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(263..283)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        438..741
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    822..870
                     /note="synthetic polyadenylation signal"
     terminator      888..934
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    939..1010
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(1162..1298)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(1311..2102,2103..2171))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1934..1953
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2917..3505
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3406..3425
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3690..3914
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3925..4719)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4036..4055)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4646..4665
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4733..4929)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4747..4882
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    4974..5045
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(5090..5226)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(5256..5302)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    5417..5538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5454..5473)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5508..5527
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    5591..6179
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5697..5708
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6106..6126
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     intron          join(10076..10451,1..100)
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 tggtcaatat gtgtaccctg ttacttctcc ccttcctatg acatgaactt aaccatagaa
       61 aagaagggga aagaaaacat caagggtccc atagactcac cctgaagttc tcaggatccg
      121 agctcggtac cacatgtaag cttcgagggg aggctggatc ggtcccggtg tcttctatgg
      181 aggtcaaaac agcgtggatg gcgtctccag gcgatctgac ggttcactaa accagctctg
      241 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
      301 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
      361 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
      421 tgccaaaacc gcatcagcat ggtaatagcg atgactaata cgtagatgta ctgccaagta
      481 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
      541 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
      601 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
      661 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
      721 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
      781 attgattact agatctggct acactcgatc agcagttaga taataaaatc gctatccatc
      841 gaagatggat gtgtgttggt tttttgtgtg tgtaacgcaa cgattgatag cataacccct
      901 tggggcctct aaacgggtct tgaggggttt tttgaattga gaggtagctg aagctgctgt
      961 tatgaccact agagggaaga agatacctgt ggagctaatg gtccaagatg tcgggagccg
     1021 cgatcgctgc caaaaaggta agctgacgat agctgacgag attcgatgcc cgacgagcaa
     1081 atgagcgcca cggctgttcg cgtaagctcg aaacgacgga gattcgatag agccagagtc
     1141 gagtttaaac gattcgggat cttaaggata aatttctggt aaggaggaca cgtatggaag
     1201 tgggcaagtt ggggaagccg tatccgttgc tgaatctggc atatgtggga gtataagacg
     1261 cgcagcgtcg catcaggcat ttttttctgc gccaatgcac ctagaatcgg ttaccaatgc
     1321 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1381 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1441 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1501 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctatcaac
     1561 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttacgcaa ggttgttgcc
     1621 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1681 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1741 ttcggtcctc caatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1801 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1861 gaatactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1921 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1981 aagcgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2041 taacccactc gcgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2101 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2161 tgaatactca tgcgaaagcc tcctgctgat cctgtctctt gatagatgtt tgcaaaagct
     2221 aggactcgat tatacgttga ccgatcagac tatcaatatc taggtttaaa cagatcggta
     2281 cgctgccgtg accaggcttg agtacaatat ctgacgattg gacttcctgg gtcccaagct
     2341 ggattccgac tggctgactg ccaagcaacg atatggatcg tcgagactcg cactgagagg
     2401 atcgttctcg gtacaccagg cgcgccccga tttcgatatt cctcggatct cggatagatc
     2461 gggattgagg atgtaggttg aacttctctc gggtgatccc gatctcggat attcgggatt
     2521 ggagtaatac ctggcgatag gcgctgccaa gcaatagcca aggcgcaagg ccggccaaga
     2581 gacttgcgat agttcgttcg agaggtagcc tgcaagatgg atcggacgaa aggtttgaag
     2641 attttccgac gagggccttg gagagttcgg aaatttaaat gcaactatgg atgaacgaaa
     2701 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2761 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2821 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2881 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2941 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3001 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3061 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3121 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3181 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3241 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3301 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3361 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3421 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3481 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3541 cttttgctca catgatttaa atgaaactcg taatcggaga acgggtgcaa tagcaaggac
     3601 cagatcgaac ttgactgacg caattctgag cgtagtcgaa acccagacaa gtcgatctcg
     3661 ccaagtagct gacttgactg gttaattaac catagagccc accgcatccc cagcatgcct
     3721 gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga
     3781 atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg
     3841 agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca
     3901 actagaaggc acagggctta catactagaa gaactcgtca agaaggcgat agaaggcgat
     3961 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     4021 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     4081 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     4141 caagcaggca tcgccgtggg tcacgacgag atcctcgccg tcgggcatcc gcgccttgag
     4201 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     4261 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     4321 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     4381 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     4441 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     4501 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgg agttcattca gggcaccgga
     4561 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     4621 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     4681 ggcgggagaa cctgcgtgca atccatcttg ttcaatcatg ttggctacct cctttgcaaa
     4741 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     4801 cctcggcctc tgcataaata aaaaaaatta gtcagcgatg gggcggagaa tgggcggaac
     4861 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     4921 tgagatgcag atcgcttaat taagtttcga tagcccaagg taaccaatga gattagggag
     4981 ggaagaaagt ttgatgaact acttctgacc cctagtggtg tccagaaaag accattaaag
     5041 gaatgtcttg atcgaaatcg agagatcgcc tgcaggatag ctgacgacct taaggataaa
     5101 tttctggtaa ggaggacacg tatggaagtg ggcaagttgg ggaagccgta tccgttgctg
     5161 aatctggcat atgtgggagt ataagacgcg cagcgtcgca tcaggcattt ttttctgcgc
     5221 caatgccggt agatagcaat ccggcccgag gggcacaaaa aacccctcaa gacccgttta
     5281 gaggccccaa ggggttatgc tacgataccg atagagatgg gccaatacca catttgtaga
     5341 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     5401 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5461 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5521 actcatcaat gtatcttatc atgtctgtga gctgaaggta cgctgtatct cagtcagtca
     5581 agctagacag gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga
     5641 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg
     5701 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg
     5761 agcagccatg gaaaggacgt cagcttcccc gacaacacca cggaattgtc agtgcccaac
     5821 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata
     5881 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca
     5941 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac
     6001 aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata
     6061 cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga
     6121 gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgatta
     6181 ggcctgcgga tccagaaatc gattgtatca gtcagtcagt gcaggaggag acaacttcta
     6241 gaggatcctt tatttacaac aagatccgca actgcaacat cctttcaagc aggaacaaca
     6301 gctagtcata cagcacaaca taattgtgac cataacgata gctataagtc cggcgataaa
     6361 gcccaaccaa atgtaccagg gccatttaat atattgttcg tatttgccca gttcttgcaa
     6421 gtcgatcaaa gattcattca agttcttggc gacctcatta aggcgatcaa tctctttctg
     6481 aatgttgacg acacttgcat ttattcctga tatgtccccg agatcaacat cgggtgaagt
     6541 atgattctta aagtatttat ccaattcctc tttgaagcta tccaactcgg gttgcagggg
     6601 atcatatacc gtgttgttca cgatcccaat gaccacatca cagttgcctg agacaaatgt
     6661 attgtcagtg gttatgattt ggggttcata gaaattcctt tgggtcacaa accaatgcgt
     6721 gccattactg acgaaaacgc cttccctggg aaagtgcgct ttgccatcgt gacagattgc
     6781 tggagctgtc gtaaagttct tctcttgagc agggacataa gtgacgtgga gaaagaccac
     6841 tccatgtggg gcggattggg ggaaggacat caagtgatag cccttgccac aaaagtcaac
     6901 ccgtttggac tgcccgagca cgcactctga cattttagtg gcggccaggt tggcgcttgc
     6961 tcgtatctca gcagcccgga tcaactgttg tgtcacataa gtttggagtg actggagcct
     7021 gcctgtgatg agccgatcaa tttgcacctc ggcttccacc ttatcaagtc tggacaatat
     7081 gtcgttgagg acgctggaga ttgcgccgaa gttagaactc agttgcttca cgagggtatt
     7141 cagggcctgg gcgttttgat tcacaacatc ttggagtttg ccgagggcgg acgctgtgct
     7201 tgacagtgaa tcttggatct tgccaatcgc gctgttaaat tgattagcga tcagcttctg
     7261 attttcatac aatacatttt gcgtgacacc aatcccattg aatctgtaag ccatttgcat
     7321 agcgaaaggt atttgaaggg ccgcacccgc gccaaaagtc cagccggatg taatggttcc
     7381 tgccaacaat gctgatgtat actgtgcaat catctcatca gtgagcagag ggggcagaac
     7441 tgtcaaccca ttgaattttt gagcacagat aaggtcgcgg gccgcaatgt ctcccaaaca
     7501 atccccatac tgcttaatga aacccgcgtc agccaacgta actttgttga acagcagatc
     7561 ctcgataaag ctgcgttttg aaggcttaga tgggtctggc agtatttggc taaaattaaa
     7621 cccgccaaaa tctttgattg gcggcgtttt gtatatttgc tttacttgtg cgaacacctc
     7681 ttgggtattc ttgtcttgtt cgaccgcaat tccggtcaat gctcggttga gttgagtaca
     7741 gaaagacccg tattgaagca agaggttgct acactcggtg gaatccccac agatatacat
     7801 cgtacagtct acgctcgtct tagtcatgct gacgggcaag atttccgtgg taacgctaat
     7861 ggtaaagtta gttgggattg cgatggaatt gtttgaataa gccacggagt tctcagcccc
     7921 gagactcatg gtataggcga ttatggactg gctggctact gagcgagcgc gccgtggtga
     7981 gttcgtttga gtttgataag acgcacagat tcctgcccca ataggaatat cacattcgta
     8041 tgaattattg acatgctcag cgccaatgag acaacccgct ctagtttgaa agacattgga
     8101 acctgttgag tagactctcc aggtgggtgt cagttggtcg gcgtgtatcg ccacagggac
     8161 ttcggtacag ttgacatctt gatacaggac agccacttgg ttggatgtgt tcgttccggg
     8221 agttatcacg ctgaccccgc cgaagctaca gggtgttata tcgaggattt ccagtgtttg
     8281 cggatcgcgg acggcatctg tggtatcggc aatgtctcta ccaaattgtt gaaaagggag
     8341 aaatttcttg tttgattctg tcagcacccc tgttccggtg agcccattaa agttgaagtt
     8401 cacacattta ttcttcacca ggttagtgct tttcttcggt ccgcaaactg tagccggtgc
     8461 gtggagcagc tcaaagctca agactaccac ccgatatggt tgatagccca ccccatttgt
     8521 tggttgaaat ccgtatgatt gaaggggaaa ataacagtta aacccttcca ctccattaca
     8581 tggggtgctg ccagcttgat aaatttcggt ggagatgtca cgttcaaagg gcttcagatt
     8641 tgacttgcga aacaggcggt aaagataatt atagttgccg ccgacttttg aatcgagatt
     8701 attggagttc catgctataa cgcagccggt gaagtcatcc ggcaacttat aattatagtc
     8761 tgctatcttt ccggtttgcc ccggtgcaat ctgtcggact tcgtcgccgc gtatgacgaa
     8821 agaatctgcg taaacgttgg taaaacacaa gtcattcaat ttcgtagggc tgaccccata
     8881 acacttaaaa gtgctgaatg atgcgctatt atacaggaca ctataatctg caacacaatt
     8941 tgatattcgc ttgcgattcc aggcataaac tgatgcaaat cgagtggcat taaatacttc
     9001 accgaaagga cacaaattgg tgatgttagg gaatcgaaca atggattcgg tgggttggac
     9061 gcggaagtta ctggtttggt atatgccttt ctcaactgtg aaggacttga gtgtgcattt
     9121 agtttcggac aggggatcga gggcacagtc tacggcatcg gtaattgttc cgttttcgtt
     9181 atatttgaga agaaaggtgc gtggttgcaa atatcccaca tagtaggctg ctgcgcccgc
     9241 tgtccaacca ctactactgt cgccaggcgt gaggtagctt cgatgaaggg caagaagggt
     9301 ttggaaccgg gtgatgttga tgccaatcgg caagtccacg aggggttcca acgcggagaa
     9361 gccttgaggg agatcccgaa ccagatttat cggggtatgc ttgctataaa ttttgaagta
     9421 gccgtctata ttcttaaaca caaattcgcg gagattcttg aagttgcctt gcttcccttc
     9481 aagatccatg agaaatggtt gggaaacgta ctcaaaagta cagttgttag cggaactgta
     9541 aacgcggaac tctgactcca tccaagactt gttattctta tgataataaa ccccgagaaa
     9601 gggatcgtta caaaattgaa attcacagac tttgatgacg acattggtgg cgttgttcac
     9661 aatgaggaga gactgtgtct tggagtccag ggtggtgcca aatatccatc cccggataat
     9721 gttggacttc tccgttgagg caaagtatac gccatcgtta aatggcaaga cgggattatc
     9781 aaatcgttta gttccgttgg tgccagaaac gtggatagca tggaaccagg tgacatttga
     9841 gaagaatggc aggaaaaggt catgagtaga atgaaggaca ctggacctaa agactttatc
     9901 gggatagtat actccacgag taaaagagtt cgtgtaagcg ggtgggagct gcgttctagt
     9961 tgtgaggttc acgcattggg aagaaacgag gggcaggaga acgaggaaca caaacatggc
    10021 ggccgccttt gccaaagtga tgggccagca cacagaccag cacgttgccc aggagctgtg
    10081 ggaggaagat aagaggtatg aacatgatta gcaaaagggc ctagcttgga ctcagaataa
    10141 tccagcctta tcccaaccat aaaataaaag cagaatggta gctggattgt agctgctatt
    10201 agcaatatga aacctcttac atcagttaca atttatatgc agaaatattt atatgcagaa
    10261 atattgctat tgccttaacc cagaaattat cactgttatt ctttagaatg gtgcaaagag
    10321 gcatgataca ttgtatcatt attgccctga aagaaagaga ttagggaaag tattagaaat
    10381 aagataaaca aaaaagtata ttaaaagaag aaagcatttt ttaaaattac aaatgcaaaa
    10441 ttaccctgat t
//
