LOCUS       sequence-446849-       12590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E 
            S19E under the spleen focus-forming virus (SFFV) promoter and 
            Emerald under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..12590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(4342..4362)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4760..4771)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5810..5829
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      6003..6012
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6009..6011,6012..6014,6015..6728)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6054..6075)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6315..6334)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6662..6683
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6823..7056
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7081..7099)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7082..7099)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7389..7408)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7622..7641)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7741..7763
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7801..7819)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7887..7991
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7992..8060,8061..8852)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8210..8229)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9023..9611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9512..9531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9765..9782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9852..9872)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9857..10186
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10037..10172
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11320..11385
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11515..11535
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11960..12094
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11997..12016)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12051..12070
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(12262..12590,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccaccg gtcgccacca tgcccgaagc caaacaaagg ggctccaagg gcggccacgg
     2461 cgccgcggaa cccgaggaga agggtgccca cccgtcgggc ggcgcggatg acgtggcgaa
     2521 gaagccgccg ccggcgccgc agcagccgcc gccgccgccc gcgccgcacc cgcagcagca
     2581 cccgcagcag cacccgcaga accaggcgca cggcaagggc ggccaccgcg gcggcggcgg
     2641 cggcggcggc aagtcctcct cctcctcctc cgcctccgcc gccgctgccg ccgccgccgc
     2701 ctcgtcctcg gcgtcctgct cgcgcaggct cggcagggcg ctcaactttc tcttctacct
     2761 cgccctggtg gcggcggccg ctttctcggg ctggtgcgtc caccacgtcc tggaggaggt
     2821 ccagcaggtc cggcgcagcc accaggactt ctcccggcag agggaggagc tgggccaggg
     2881 cttgcagggc gtcgagcaga aggtgcagtc tttgcaagcc acatttggaa cttttgagtc
     2941 catcttgaga agctcccaac ataaacaaga cctcacagag aaagctgtga agcaagggga
     3001 gagtgaggtc agccggatca gcgaagtgct gcagaaactc cagaatgaga ttctcaaaga
     3061 cctctcggat gggatccatg tggtgaagga cgcccgggag cgggacttca cgtccctgga
     3121 gaacacggtg gaggagcggc tgacggagct caccaaatcc atcaacgaca acatcgccat
     3181 cttcacagaa gtccagaaga ggagccagaa ggagatcaat gacatgaagg caaaggttgc
     3241 ctccctggaa gaatctgagg ggaacaagca ggatttgaaa gccttaaagg aagctgtgaa
     3301 ggagatacag acctcagcca agtccagaga gtgggacatg gaggccctga gaagtaccct
     3361 tcagactatg gagtctgaca tctacaccga ggtccgcgag ctggtgagcc tcaagcagga
     3421 gcagcaggct ttcaaggagg cggccgacac ggagcggctc gccctgcagg ccctcacgga
     3481 gaagcttctc aggtctgagg agtccgtctc ccgcctcccg gaggagatcc ggagactgga
     3541 ggaagagctc cgccagctga agtccgattc ccacgggccg aaggaggacg gaggcttcag
     3601 acactcggaa gcctttgagg cactccagca aaagagtcag ggactggact ccaggctcca
     3661 gcacgtggag gatggggtgc tctccatgca ggtggcttct gcgcgccaga ccgagagcct
     3721 ggagtccctc ctgtccaaga gccaggagca cgagcagcgc ctggccgccc tgcaggggcg
     3781 cctggaaggc ctcgggtcct cagaggcaga ccaggatggc ctggccagca cggtgaggag
     3841 cctgggcgag acccagctgg tgctctacgg tgacgtggag gagctgaaga ggagtgtggg
     3901 cgagctcccc agcaccgtgg aatcactcca gaaggtgcag gagcaggtgc acacgctgct
     3961 cagtcaggac caagcccagg ccgcccgtct gcctcctcag gacttcctgg acagactttc
     4021 ttctctagac aacctgaaag cctcagtcag ccaagtggag gcggacttga aaatgctcag
     4081 gactgctgtg gacagtttgg ttgcatactc ggtcaaaata gaaaccaacg agaacaatct
     4141 ggaatcagcc aagggtttac tagatgacct gaggaatgat ctggataggt tgtttgtgaa
     4201 agtggagaag attcacgaaa aggtctaaag cggccgcgac tctagagtcg acctgcaggc
     4261 atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     4321 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4381 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4441 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4501 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4561 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4621 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4681 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4741 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcgggcgccc
     4801 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
     4861 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
     4921 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
     4981 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
     5041 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
     5101 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
     5161 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
     5221 ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
     5281 aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga
     5341 cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag
     5401 aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct
     5461 ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc
     5521 gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg
     5581 agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg
     5641 ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc
     5701 ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg
     5761 aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg
     5821 ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt
     5881 gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct
     5941 aaattctggc cgtttttggc ttttttgtta gacgaagctt ggtaccgagc tcggatcaat
     6001 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     6061 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     6121 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     6181 ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc taccccgacc
     6241 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     6301 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     6361 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     6421 tggggcacaa gctggagtac aactacaaca gccacaaggt ctatatcacc gccgacaagc
     6481 agaagaacgg catcaaggtg aacttcaaga cccgccacaa catcgaggac ggcagcgtgc
     6541 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     6601 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     6661 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     6721 acaagtaact cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg
     6781 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     6841 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     6901 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6961 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7021 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat
     7081 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
     7141 gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac
     7201 cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt
     7261 ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc
     7321 tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     7381 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     7441 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     7501 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     7561 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca
     7621 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     7681 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     7741 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     7801 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     7861 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7921 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7981 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8041 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8101 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8161 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8221 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8281 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8341 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8401 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8461 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8521 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8581 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8641 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8701 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8761 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8821 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8881 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8941 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9001 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9061 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9781 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9841 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     9901 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     9961 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
    10021 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
    10081 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    10141 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
    10201 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
    10261 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
    10321 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
    10381 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
    10441 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
    10501 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
    10561 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
    10621 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
    10681 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
    10741 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
    10801 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
    10861 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
    10921 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
    10981 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
    11041 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
    11101 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
    11161 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
    11221 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
    11281 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
    11341 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
    11401 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
    11461 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
    11521 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
    11581 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
    11641 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
    11701 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
    11761 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
    11821 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
    11881 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    11941 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    12001 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    12061 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
    12121 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
    12181 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
    12241 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
    12301 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
    12361 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
    12421 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
    12481 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
    12541 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
//
