LOCUS       sequence-44685-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: TALE10
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..58)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(80..229)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(254..274)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctactgggcc aggattctcc tcgacgtcac cgcatgttag cagacttcct ctgccctctc
       61 cactgcctct agagttaatc agcatgtcca ggtcgaaatc atcaagggcg tcactgccga
      121 gcatgtcgag gtcaaagtca tcaagggcat ccgaaccaag catgtcaagg tcaaaatcat
      181 cgagggcgtc acttcccagc atatccagat caaaatcgtc caatgcgtca gcccgtccgg
      241 aaccgctggc ctccaccttt ctcttcttct tggggctagc tgacgcgcga gtttggtccc
      301 cttcatgcat gggcgagggc gcgtccaaat ccctctccag cgagtctgcg aacgcatgaa
      361 ggctcgcctg gtcaggtgtc tgagttgacg taggtgaagg cttcgcgcgt ttcataccgc
      421 tcgcttggag aatcctgtcc caccgctggg aggcgggagg cagtgtgccc gagcgggcct
      481 cgagttctgt gacaccgact ctacgaaaga gctgcagcag tccgtgtctc gacataccaa
      541 attgagtcat ggcgtcatcg aacgcttgtg cggggtggga gtgacactgg aagaatccga
      601 gcacgcggac cacttgcgcg tgatctgcca ctcgatgtga tgtcctctcg ggaatccttc
      661 tgttggtccg cttaatcaat gcaggcgcgt gcgggagccc cttcttcacc gcatccaagg
      721 cgggtcgtcc gccgaggcag gccagcgcta caagatgatc attagtgagt gcggccagcg
      781 cggggtccgg gctcgaaagc tg
//
