LOCUS       sequence-446852-       12593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human CKAP4 mutation double 
            cysteine (CC) under the spleen focus-forming virus (SFFV) promoter 
            and Emerald under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12593)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-CKAP4 CC-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..12593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(4345..4365)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4763..4774)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5813..5832
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      6006..6015
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6012..6014,6015..6017,6018..6731)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6057..6078)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6318..6337)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6665..6686
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6826..7059
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7084..7102)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7085..7102)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7392..7411)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7625..7644)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7744..7766
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7804..7822)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7890..7994
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7995..8063,8064..8855)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8213..8232)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9026..9614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9515..9534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9768..9785
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9855..9875)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9860..10189
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10040..10175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11323..11388
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11518..11538
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11963..12097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12000..12019)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12054..12073
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(12265..12593,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccaccg gtcgccacca tgccctcggc caaacaaagg ggctccaagg gcggccacgg
     2461 cgccgcgagc ccctcggaga agggtgccca cccgtcgggc ggcgcggatg acgtggcgaa
     2521 gaagccgccg ccggcgccgc agcagccgcc gccgccgccc gcgccgcacc cgcagcagca
     2581 cccgcagcag cacccgcaga accaggcgca cggcaagggc ggccaccgcg gcggcggcgg
     2641 cggcggcggc aagtcctcct cctcctcctc cgcctccgcc gccgctgccg ccgccgccgc
     2701 ctcgtcctcg gcgtcctgct gttcgcgcag gctcggcagg gcgctcaact ttctcttcta
     2761 cctcgccctg gtggcggcgg ccgctttctc gggctggtgc gtccaccacg tcctggagga
     2821 ggtccagcag gtccggcgca gccaccagga cttctcccgg cagagggagg agctgggcca
     2881 gggcttgcag ggcgtcgagc agaaggtgca gtctttgcaa gccacatttg gaacttttga
     2941 gtccatcttg agaagctccc aacataaaca agacctcaca gagaaagctg tgaagcaagg
     3001 ggagagtgag gtcagccgga tcagcgaagt gctgcagaaa ctccagaatg agattctcaa
     3061 agacctctcg gatgggatcc atgtggtgaa ggacgcccgg gagcgggact tcacgtccct
     3121 ggagaacacg gtggaggagc ggctgacgga gctcaccaaa tccatcaacg acaacatcgc
     3181 catcttcaca gaagtccaga agaggagcca gaaggagatc aatgacatga aggcaaaggt
     3241 tgcctccctg gaagaatctg aggggaacaa gcaggatttg aaagccttaa aggaagctgt
     3301 gaaggagata cagacctcag ccaagtccag agagtgggac atggaggccc tgagaagtac
     3361 ccttcagact atggagtctg acatctacac cgaggtccgc gagctggtga gcctcaagca
     3421 ggagcagcag gctttcaagg aggcggccga cacggagcgg ctcgccctgc aggccctcac
     3481 ggagaagctt ctcaggtctg aggagtccgt ctcccgcctc ccggaggaga tccggagact
     3541 ggaggaagag ctccgccagc tgaagtccga ttcccacggg ccgaaggagg acggaggctt
     3601 cagacactcg gaagcctttg aggcactcca gcaaaagagt cagggactgg actccaggct
     3661 ccagcacgtg gaggatgggg tgctctccat gcaggtggct tctgcgcgcc agaccgagag
     3721 cctggagtcc ctcctgtcca agagccagga gcacgagcag cgcctggccg ccctgcaggg
     3781 gcgcctggaa ggcctcgggt cctcagaggc agaccaggat ggcctggcca gcacggtgag
     3841 gagcctgggc gagacccagc tggtgctcta cggtgacgtg gaggagctga agaggagtgt
     3901 gggcgagctc cccagcaccg tggaatcact ccagaaggtg caggagcagg tgcacacgct
     3961 gctcagtcag gaccaagccc aggccgcccg tctgcctcct caggacttcc tggacagact
     4021 ttcttctcta gacaacctga aagcctcagt cagccaagtg gaggcggact tgaaaatgct
     4081 caggactgct gtggacagtt tggttgcata ctcggtcaaa atagaaacca acgagaacaa
     4141 tctggaatca gccaagggtt tactagatga cctgaggaat gatctggata ggttgtttgt
     4201 gaaagtggag aagattcacg aaaaggtcta aagcggccgc gactctagag tcgacctgca
     4261 ggcatgcaag cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     4321 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4381 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4441 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4501 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4561 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4621 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4681 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4741 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcgggcg
     4801 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc
     4861 cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc
     4921 agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct
     4981 ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat
     5041 ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag
     5101 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
     5161 tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg
     5221 gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc
     5281 ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga
     5341 agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc
     5401 aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg
     5461 gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt
     5521 gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt
     5581 gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg
     5641 tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg
     5701 ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa
     5761 gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc
     5821 cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct
     5881 tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc
     5941 gctaaattct ggccgttttt ggcttttttg ttagacgaag cttggtaccg agctcggatc
     6001 aattcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     6061 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     6121 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     6181 cctggcccac cctcgtgacc accttgacct acggcgtgca gtgcttcgcc cgctaccccg
     6241 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     6301 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     6361 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     6421 tcctggggca caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca
     6481 agcagaagaa cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg
     6541 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     6601 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     6661 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     6721 tgtacaagta actcgaggga attaattcga gctcggtacc tttaagacca atgacttaca
     6781 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6841 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6901 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6961 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7021 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg
     7081 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
     7141 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
     7201 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
     7261 agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat
     7321 cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     7381 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     7441 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     7501 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     7561 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc
     7621 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     7681 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7741 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     7801 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7861 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7921 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7981 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8041 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8101 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8161 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8221 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8281 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8341 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8401 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8461 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8521 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8581 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8641 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8701 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8761 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8821 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8881 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8941 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9001 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9061 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9121 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9181 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9241 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9301 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9361 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9421 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9481 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9541 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9661 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9721 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9781 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9841 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     9901 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     9961 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
    10021 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    10081 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    10141 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
    10201 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
    10261 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
    10321 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
    10381 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
    10441 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
    10501 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
    10561 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
    10621 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
    10681 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
    10741 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
    10801 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
    10861 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
    10921 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
    10981 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
    11041 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
    11101 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
    11161 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
    11221 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
    11281 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
    11341 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
    11401 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
    11461 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
    11521 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
    11581 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
    11641 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
    11701 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
    11761 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
    11821 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
    11881 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    11941 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    12001 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    12061 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    12121 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    12181 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    12241 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
    12301 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
    12361 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
    12421 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
    12481 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
    12541 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgc
//
