LOCUS       sequence-446853-       11752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human calumenin (CALU) under 
            the spleen focus-forming virus (SFFV) promoter and Emerald under the
            Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-CALU-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..11752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(3504..3524)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3922..3933)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4972..4991
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5165..5174
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5171..5173,5174..5176,5177..5890)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5216..5237)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5477..5496)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5824..5845
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5985..6218
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6243..6261)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6244..6261)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6551..6570)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6784..6803)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6903..6925
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6963..6981)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7049..7153
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7154..7222,7223..8014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7372..7391)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8185..8773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8674..8693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8927..8944
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9014..9034)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9019..9348
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9199..9334
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10482..10547
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10677..10697
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11122..11256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11159..11178)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11213..11232
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11424..11752,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 gatccaccgg tcgccaccat gaaggaaact gatctaatta tcatggacct gcgacagttt
     2461 cttatgtgcc tgtccctgtg cacagccttt gccttgagca aacccacaga aaagaaggac
     2521 cgtgtacatc atgagcctca gctcagtgac aaggttcaca atgatgctca gagttttgat
     2581 tatgaccatg atgccttctt gggtgctgaa gaagcaaaga cctttgatca gctgacacca
     2641 gaagagagca aggaaaggct tggaaagatt gtaagtaaaa tagatggcga caaggacggg
     2701 tttgtcactg tggatgagct caaagactgg attaaatttg cacaaaagcg ctggatttac
     2761 gaggatgtag agcgacagtg gaaggggcat gacctcaatg aggacggcct cgtttcctgg
     2821 gaggagtata aaaatgccac ctacggctac gttttagatg atccagatcc tgatgatgga
     2881 tttaactata aacagatgat ggttagagat gagcggaggt ttaaaatggc agacaaggat
     2941 ggagacctca ttgccaccaa ggaggagttc acagctttcc tgcaccctga ggagtatgac
     3001 tacatgaaag atatagtagt acaggaaaca atggaagata tagataagaa tgctgatggt
     3061 ttcattgatc tagaagagta tattggtgac atgtacagcc atgatgggaa tactgatgag
     3121 ccagaatggg taaagacaga gcgagagcag tttgttgagt ttcgggataa gaaccgtgat
     3181 gggaagatgg acaaggaaga gaccaaagac tggatccttc cctcagacta tgatcatgca
     3241 gaggcagaag ccaggcacct ggtctatgaa tcagaccaaa acaaggatgg caagcttacc
     3301 aaggaggaga tcgttgacaa gtatgactta tttgttggca gccaggccac agattttggg
     3361 gaggccttag tacggcatga tgagttctga agcggccgcg actctagagt cgacctgcag
     3421 gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     3481 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3541 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3601 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3661 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3721 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3781 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3841 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3901 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcgggcgc
     3961 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     4021 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     4081 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     4141 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     4201 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     4261 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     4321 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     4381 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     4441 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     4501 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     4561 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     4621 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     4681 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     4741 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     4801 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     4861 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     4921 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     4981 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     5041 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     5101 ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttggtaccga gctcggatca
     5161 attcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     5221 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     5281 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     5341 ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag tgcttcgccc gctaccccga
     5401 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     5461 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     5521 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     5581 cctggggcac aagctggagt acaactacaa cagccacaag gtctatatca ccgccgacaa
     5641 gcagaagaac ggcatcaagg tgaacttcaa gacccgccac aacatcgagg acggcagcgt
     5701 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     5761 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     5821 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     5881 gtacaagtaa ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa
     5941 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6001 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     6061 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6121 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6181 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     6241 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     6301 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     6361 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     6421 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     6481 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     6541 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     6601 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     6661 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     6721 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     6781 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     6841 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     6901 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     6961 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     7021 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7081 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7141 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7201 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7261 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7321 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7381 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     7441 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7501 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7561 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7621 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7681 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7741 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7801 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7861 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     7921 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     7981 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8041 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8101 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8161 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8221 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8281 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8341 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8401 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8461 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8521 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8581 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8641 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8701 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8761 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8821 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8881 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     8941 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9001 ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     9061 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9121 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9181 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9241 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9301 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac
     9361 aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag
     9421 tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa
     9481 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
     9541 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
     9601 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
     9661 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
     9721 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
     9781 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
     9841 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
     9901 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
     9961 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
    10021 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
    10081 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
    10141 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
    10201 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
    10261 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
    10321 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
    10381 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
    10441 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
    10501 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
    10561 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
    10621 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
    10681 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
    10741 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
    10801 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
    10861 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
    10921 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
    10981 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
    11041 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    11101 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    11161 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    11221 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
    11281 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
    11341 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
    11401 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
    11461 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
    11521 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
    11581 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
    11641 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
    11701 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gc
//
