LOCUS       sequence-446855-       11743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human calumenin (CALU) under 
            the spleen focus-forming virus (SFFV) promoter and mCherry under the
            Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-CALU-WPRE-UbC-mCherry
FEATURES             Location/Qualifiers
     source          1..11743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(3504..3524)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3922..3933)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4972..4991
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5165..5174
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5171..5881
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5316..5334)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5584..5603
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             5976..6209
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6234..6252)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6235..6252)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6542..6561)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6775..6794)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6894..6916
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6954..6972)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7040..7144
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7145..7213,7214..8005)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7363..7382)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8176..8764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8665..8684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8918..8935
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9005..9025)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9010..9339
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9190..9325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10473..10538
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10668..10688
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11113..11247
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11150..11169)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11204..11223
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11415..11743,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag ctgaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 gatccaccgg tcgccaccat gaaggaaact gatctaatta tcatggacct gcgacagttt
     2461 cttatgtgcc tgtccctgtg cacagccttt gccttgagca aacccacaga aaagaaggac
     2521 cgtgtacatc atgagcctca gctcagtgac aaggttcaca atgatgctca gagttttgat
     2581 tatgaccatg atgccttctt gggtgctgaa gaagcaaaga cctttgatca gctgacacca
     2641 gaagagagca aggaaaggct tggaaagatt gtaagtaaaa tagatggcga caaggacggg
     2701 tttgtcactg tggatgagct caaagactgg attaaatttg cacaaaagcg ctggatttac
     2761 gaggatgtag agcgacagtg gaaggggcat gacctcaatg aggacggcct cgtttcctgg
     2821 gaggagtata aaaatgccac ctacggctac gttttagatg atccagatcc tgatgatgga
     2881 tttaactata aacagatgat ggttagagat gagcggaggt ttaaaatggc agacaaggat
     2941 ggagacctca ttgccaccaa ggaggagttc acagctttcc tgcaccctga ggagtatgac
     3001 tacatgaaag atatagtagt acaggaaaca atggaagata tagataagaa tgctgatggt
     3061 ttcattgatc tagaagagta tattggtgac atgtacagcc atgatgggaa tactgatgag
     3121 ccagaatggg taaagacaga gcgagagcag tttgttgagt ttcgggataa gaaccgtgat
     3181 gggaagatgg acaaggaaga gaccaaagac tggatccttc cctcagacta tgatcatgca
     3241 gaggcagaag ccaggcacct ggtctatgaa tcagaccaaa acaaggatgg caagcttacc
     3301 aaggaggaga tcgttgacaa gtatgactta tttgttggca gccaggccac agattttggg
     3361 gaggccttag tacggcatga tgagttctga agcggccgcg actctagagt cgacctgcag
     3421 gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     3481 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3541 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3601 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3661 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3721 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3781 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3841 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3901 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcgggcgc
     3961 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     4021 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     4081 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     4141 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     4201 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     4261 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     4321 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     4381 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     4441 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     4501 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     4561 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     4621 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     4681 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     4741 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     4801 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     4861 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     4921 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     4981 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     5041 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     5101 ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttggtaccga gctcggatca
     5161 attcgccacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     5221 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     5281 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     5341 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     5401 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     5461 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     5521 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     5581 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     5641 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     5701 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     5761 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     5821 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     5881 actcgaggga attaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
     5941 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6001 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     6061 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6121 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6181 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
     6241 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     6301 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
     6361 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
     6421 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
     6481 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     6541 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     6601 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     6661 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     6721 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     6781 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     6841 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     6901 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     6961 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     7021 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7081 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7141 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7201 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7261 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7321 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7381 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7441 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7501 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7561 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7621 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7681 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7741 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7801 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7861 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7921 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7981 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8041 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8101 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8161 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8221 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8281 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8341 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8401 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8461 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8521 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8581 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8641 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8701 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8761 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8821 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8881 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8941 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9001 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     9061 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     9121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     9181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
     9361 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     9421 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     9481 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     9541 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     9601 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
     9661 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     9721 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     9781 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     9841 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     9901 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     9961 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
    10021 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
    10081 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
    10141 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
    10201 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
    10261 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
    10321 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
    10381 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
    10441 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
    10501 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
    10561 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
    10621 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
    10681 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
    10741 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
    10801 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
    10861 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
    10921 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
    10981 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
    11041 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    11101 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    11161 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    11221 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    11281 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    11341 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    11401 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
    11461 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
    11521 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
    11581 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
    11641 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
    11701 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgc
//
