LOCUS       sequence-446858-       12265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human lunapark (LNPK) under the
            spleen focus-forming virus (SFFV) promoter and Emerald under the 
            Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12265)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-LNPK-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..12265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2413..2422
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4017..4037)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4435..4446)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5485..5504
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5678..5687
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5684..5686,5687..5689,5690..6403)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5729..5750)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5990..6009)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6337..6358
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6498..6731
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6756..6774)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6757..6774)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7064..7083)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7297..7316)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7416..7438
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7476..7494)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7562..7666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7667..7735,7736..8527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7885..7904)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8698..9286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9187..9206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9440..9457
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9527..9547)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9532..9861
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9712..9847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10995..11060
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11190..11210
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11635..11769
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11672..11691)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11726..11745
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11937..12265,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 gatccaccgg tcgccaccat gggaaggagt tggcccggct tgtctctctg tcagtggcgg
     2461 ctgctgcctg ctctggaggc aggctgggcg gtggcggccg agactggcgg gggtggacgc
     2521 ccgggccggg ctgcgcccgc ttcttgcagc tgtgaattcc tttggacaat tgatgatatt
     2581 tatcattgtg cccagtttct acaaataaaa gatgggtgga ttattttctc gatggagggt
     2641 aagacaaaac cttcaactgt agaagttcta gaaagtatag ataaggaaat tcaagcattg
     2701 gaagaattta gggaaaaaaa tcagagatta caaaaattat gggttggaag attaattctg
     2761 tattcctcag ttctctatct gtttacatgc ttaattgtat atttgtggta tcttcctgat
     2821 gaatttacag caagacttgc catgacactc ccattttttg cttttccatt gatcatctgg
     2881 agcataagaa cagtaattat tttcttcttt tccaagagaa cagaaagaaa taatgaagca
     2941 ttggatgatt taaaatccca gaggaaaaaa atacttgaag aagtcatgga aaaagaaact
     3001 tacaagacgg ctaaattaat tcttgaaagg tttgatccgg actcaaagaa agcaaaggag
     3061 tgtgagccgc catctgctgg agcagctgta actgcaagac ctggacaaga gattcgtcag
     3121 cgaactgcag ctcaaagaaa cctttctcca acaccagcaa gccctaacca gggccctcct
     3181 ccacaagttc cagtatctcc tggaccacca aaggacagtt ctgcccctgg tggaccccca
     3241 gaaaggactg ttactccagc cctatcatca aatgtgttac caagacatct tggatcccct
     3301 gctacttcag tgcctggaat gggtcttcat cctccaggtc cacctttagc aagacctatt
     3361 ctcccccgag aacgaggtgc tttggataga attgttgaat atttggttgg tgatggtcca
     3421 caaaacaggt atgcacttat atgtcagcag tgtttttctc ataatggcat ggctttgaag
     3481 gaagaatttg aatacattgc ttttcgatgt gcctactgtt ttttcttgaa ccctgcaaga
     3541 aaaaccagac ctcaggctcc aagacttcct gagtttagtt ttgagaagag gcaggtggtg
     3601 gaaggttcaa gttcagttgg tcccttgcca tcaggaagtg tgctttcatc agacaaccag
     3661 tttaatgaag aatctttaga acacgatgtt cttgatgata atacagagca gacagatgac
     3721 aaaataccag ctacagaaca gacaaaccaa gtgattgaaa aagcatctga ctcagaggaa
     3781 ccagaggaga aacaagagac tgagaatgag gaagcctcag tgattgaaac caactccaca
     3841 gttcctggag ctgattctat tcctgatcct gaactaagtg gagaatcttt gacggcagag
     3901 tagagcggcc gcgactctag agtcgacctg caggcatgca agcttgatat caagcttatc
     3961 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4021 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4081 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4141 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4201 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4261 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4321 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4381 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4441 ctcaatccag cggaccttcc ttcccgcggg cgcccccctc ctcacggcga gcgctgccac
     4501 gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc
     4561 ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg
     4621 ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa
     4681 gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt
     4741 atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg
     4801 gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc
     4861 ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag
     4921 ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc
     4981 aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc
     5041 gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct
     5101 aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg
     5161 tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg
     5221 gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga
     5281 cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt
     5341 aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac
     5401 aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt
     5461 tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg
     5521 cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat
     5581 gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt
     5641 tgttagacga agcttggtac cgagctcgga tcaattcgcc accatggtga gcaagggcga
     5701 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     5761 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     5821 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac
     5881 ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     5941 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     6001 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     6061 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     6121 caacagccac aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt
     6181 caagacccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     6241 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     6301 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     6361 cgccgccggg atcactctcg gcatggacga gctgtacaag taactcgagg gaattaattc
     6421 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6481 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     6541 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6601 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6661 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6721 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     6781 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     6841 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     6901 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     6961 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     7021 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     7081 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     7141 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     7201 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     7261 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     7321 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     7381 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     7441 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     7501 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7561 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7621 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7681 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7741 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7801 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7861 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7921 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7981 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8041 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8101 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8161 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8221 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8281 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8341 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8401 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8461 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8521 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8581 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8641 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8701 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8761 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8821 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8881 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8941 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9001 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9061 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9121 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9181 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9241 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9301 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9361 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9421 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9481 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     9541 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     9601 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9661 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     9721 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9781 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     9841 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
     9901 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
     9961 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
    10021 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
    10081 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
    10141 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
    10201 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
    10261 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
    10321 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
    10381 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
    10441 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
    10501 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
    10561 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
    10621 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
    10681 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
    10741 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
    10801 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
    10861 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
    10921 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
    10981 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
    11041 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
    11101 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
    11161 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
    11221 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
    11281 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
    11341 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
    11401 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
    11461 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
    11521 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
    11581 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    11641 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    11701 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    11761 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    11821 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    11881 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
    11941 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
    12001 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
    12061 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
    12121 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
    12181 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
    12241 gcatccggag tacttcaaga actgc
//
