LOCUS       sequence-446859-        9468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9468)
  AUTHORS   Honnell V, Sweeney S, Norrie J, Parks M, Ramirez C, Jannu AJ, Xu B, 
            Teubner B, Lee AY, Bell C, Dyer MA
  TITLE     Evolutionary conservation of VSX2 super-enhancer modules in retinal 
            development.
  JOURNAL   Development. 2024 Jul 1;151(13):dev202435. doi: 10.1242/dev.202435. 
            Epub 2024 Jul 12.
  PUBMED    38994775
REFERENCE   2  (bases 1 to 9468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2024-07-1"; volume: "151(13):dev202435. doi";
            pages: " 10.1242/dev.202435. Epub 2024 Jul 12"
COMMENT     Sequence Label: Vsx2 hR2-20 +SV40 base prom-GFP-IRES-AP
FEATURES             Location/Qualifiers
     source          1..9468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    124..676
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    126..699
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(286..305)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     515..532
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(725..1438,1439..1441,1442..1444))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(728..1438,1439..1441,1442..1444))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(770..791)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1119..1138
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1378..1399
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1441..1450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1485..1681)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1499..1634
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4634..4738
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4739..4807,4808..5599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4957..4976)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5770..6358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6259..6278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6512..6529
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6597..6731
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6621..6640)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6675..6694
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(6737..6932)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6751..6886
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    6991..7012
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7027..7032,7033..7050,7051..7057)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7065..7081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7070..7092
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7089..7105
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7089..7105
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7448..7467
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    7466..7521
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     7567..7586
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(8081..9468,1..112))
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="LLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPA
                     QTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKH
                     VPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVT
                     TTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKY
                     MFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHL
                     MGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRA
                     YRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKAR
                     DRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFAR
                     GPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
ORIGIN
        1 cacccagggc ctcggctgcc tcgcggttcc agaagtccgg gttctcctcc tcaactggga
       61 tgatgcccag ggagagctgt agcctcaggc ccagcagcag cagcagcagc agcctcgacc
      121 ccatattatc atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga
      181 cgttttttta acctcgacta aacacatgta aagcatgtgc accgaggccc cagatcagat
      241 cccatacaat ggggtacctt ctgggcatcc ttcagcccct tgttgaatac gcttgaggag
      301 agccatttga ctctttccac aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc
      361 tttgcaggtg tatcttatac acgtggcttt tggccgcaga ggcacctgtc gccaggtggg
      421 gggttccgct gcctgcaaag ggtcgctaca gacgttgttt gtcttcaaga agcttccaga
      481 ggaactgctt ccttcacgac attcaacaga ccttgcattc ctttggcgag aggggaaaga
      541 cccctaggaa tgctcgtcaa gaagacaggg ccaggtttcc gggccctcac attgccaaaa
      601 gacggcaata tggtggaaaa taacatatag acaaacgcac accggcctta ttccaagcgg
      661 cttcggccag taacgttagg ggggggggag ggagaggggg gggggggggg cggaattggc
      721 cgctttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
      781 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca
      841 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
      901 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga
      961 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
     1021 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
     1081 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
     1141 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
     1201 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca
     1261 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
     1321 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
     1381 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
     1441 ccatggtggc gaccggtgga tcccgggccc gcggtaccaa gctttttgca aaagcctagg
     1501 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc
     1561 tctgcataaa taaaaaaaat tagtcagcga tggggcggag aatgggcgga actgggcgga
     1621 gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc
     1681 agatcgcaga tctcgagatt taattctcac aaaatcctat ggagtaggta ctattattat
     1741 atacacccat tgtatagatg aggaaactga ggcacagaga aattagtaac atgccccacc
     1801 caggtcatac agcaataaga ggtggacctg ggatgcatac gcaggcagtc tgactccaaa
     1861 ctgctcctct taaccacttt gttctgtctc catctatcca cctttaccta aaattttgtc
     1921 cttctctata ttctccacag caatgacttg ccttattttt aaaggcataa tcacagtgtg
     1981 tttggggagc aaagaaagga gcgcataacc tacactaggg caatcacaga ggctttttgg
     2041 aggtggtgtc tttatttgtt caatgatgtg gtagagcaac cactgtgtgc ccaacactgg
     2101 tgatacagac atgaagaagc catggagctc acaaacgggg gcctgaacac cccatcggca
     2161 gtgctcagtg cactgatgga gaaaacacag ggcatcgtag aaacactggg gacaccccaa
     2221 agcccaggag atcagggaag acttcctgga ggagaggcac tagtcccctt cactagatct
     2281 agtcattaac ctgaggttca gatggcccag aggtcccact gggcacactc acccttcttt
     2341 gtctcagcca gtccatcctc cgtggaccgt ctggaccaac caagcttgag accctctctg
     2401 accacctgtc ctagacaggt agaccccctc aggggaagtc tgtgttccct actgctatgt
     2461 gttcttagag gcggggcctg agcaggaacg aaactcctga accccagatc ctatgcctag
     2521 cagggtctgg tgtaagtcat tggctgcctt gggaaagccc agtggtttga ttcaaggatg
     2581 actagaattt agagcaaaaa gggaccccag aagccaccca aacctgtttt gaaggaagcc
     2641 acaggagatg cccacgcccc acagctcaga gatgctgcaa attccagcct cccagtgccc
     2701 tatcttctct ctggggtggt ctagccccac ctggcactcc cccagtcttt gtccccgtgt
     2761 ctgccttgcc tgatggtggc cagggggcct ttccggaata acgatggtat tggctcagat
     2821 taagggcaac tagctggctc ttggcttttc atttttatat aacaactaat ctccttctaa
     2881 tcagccatta ttaaattata ttccattttt aattcattca cagatcaccg ttgggacagc
     2941 tggatggata attggagaga gataatttat gtaaatgagg agcagcagaa ggtgggggag
     3001 agggaggccc agggcgccaa acggagcccc ctctgtgtcc aggctgggga gggggcagtg
     3061 gcctggctcc tcctggaaac ccgcgcctgg ggatgctggg aggattgggg gctgccatgg
     3121 gacctcaggg ctcctgaccg gtggttcctg gtgtggcccc tcagcatcac cttgaacact
     3181 tttggcaaat gggaagctca ctactttggg ggcggagtgg ctctcagaca tcagctcccc
     3241 cttttagaag cagatcctga gaggcagaat gccccagaaa attctggagt cagagctgtt
     3301 tttgattcca actccaccac tgtgtgccgt ttcctaacct tagttcccac gtctgcaaaa
     3361 cgcaggtaat gacagtatct acctcataga gtgctgtgaa gcttaagtgc ctggcaccta
     3421 aggaagagac caagacaagt acatggtggc tcttatcacc cttataggac agtgtgtatc
     3481 tttcagaatc ttcctctcta acatctgcct tctggccatg gagcttcctt ctggtaacac
     3541 acaaaagaaa tccacttcct ttgcatatct gaagacagct ctgtaccact gataataaca
     3601 ctccggaggc ttgtgtctca ttgtgtcctc cgtgtctcat agtgtctcat tgtatcctcg
     3661 gaacgactat agaaattggg ggcgattgcg tccattttac agatgaatat actgtggacc
     3721 caagctctct cctgccaatt ccttcaacaa aaacgataac aagccccaga agcctgctta
     3781 gaaaggaaca cgaggctcag aaaacctgga tcccagccgg aggctatttt gggaaggtgc
     3841 cttggggact ggcaggcagg gtcagggatg gcagggaagg tgggtgagag aggttggacc
     3901 tccacccttc ttggctgtcc ctttggggcc ccaagaatca ggcacgatgg gcattcatgg
     3961 gcacaggtgg tctgacagag cttgaggggc aaggtctgcc tgtgcctggg gctggcatgg
     4021 gctggcaggg aggcccttgg accattccca gagggagaca ggggattaag gggggttagg
     4081 gaggtggtcc cagagggtcc atgcagagta atgggattag ctcttgggca gtgagtgaaa
     4141 gttattatcg tgtggggcct ctggcctccc acccaaggct gcagctgtca cccatcctgg
     4201 gaggcaccaa cagccccctc cacccccacc ttggggcagg ctggcacctc ttctccccac
     4261 aggcttttct gctgtggtca tctgcctagg ggctggccgc ctagctttag cctcctgtcg
     4321 gtccttggca gactgggcca cccacacagt atgctctaga cttgctctgt gcctcggttt
     4381 ccccttgaac agaaagatac aagaaggtgc ttgagatcct ctgaggcatg ggggttcgga
     4441 gccaggcccc ctgggtttcc ggctctgcca cgcattcgct gtgtgaccct aggtacatct
     4501 gtcagcctct ctgtgtccta attggtaaaa tgggggcagt agcagagcag cgttagggtt
     4561 ttcatagcgg agggctgggt agggagcata gggtcggcct gtcgacccag gtggcacttt
     4621 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4681 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4741 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4801 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4861 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4921 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4981 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5041 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5101 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5161 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5221 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5281 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5341 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5401 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5461 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5521 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5581 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5641 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5701 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5761 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5821 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5881 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5941 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6001 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6061 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6121 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6181 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6241 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6301 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6361 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6421 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6481 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6541 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcggatc
     6601 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     6661 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     6721 ataaacaagt tagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     6781 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     6841 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     6901 gcgggactat ggttgctgac taattgagat gcggatccgc tggcacgaca ggtttcccga
     6961 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     7021 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     7081 atttcacaca ggaaacagct atgaccatga ttacgccaag cttgggctgc aggtcgaggg
     7141 atctccataa gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc
     7201 tggctagtaa aacatgtaag gaaaatttta gggatgttaa agaaaaaaat aacacaaaac
     7261 aaaatataaa aaaaatctaa cctcaagtca aggcttttct atggaataag gaatggacag
     7321 cagggggctg tttcatatac tgatgacctc tttatagcca acctttgttc atggcagcca
     7381 gcatatgggc atatgttgcc aaactctaaa ccaaatactc attctgatgt tttaaatgat
     7441 ttgccctccc atatgtcctt ccgagtgaga gacacaaaaa attccaacac actattgcaa
     7501 tgaaaataaa tttcctttat tagccagaag tcagatgctc aaggggcttc atgatgtccc
     7561 cataattttt ggcagaggga aaaagatctc agtggtattt gtgagccagg gcattggcca
     7621 caccagccac caccttctga taggcagcct gcacctgagg agtgcggccg gccgcgatat
     7681 cctcgacccc actgggctgt aggtggtggc tggatggcct gagccccctc ctttcctggg
     7741 gtgcaggcag ccacagggca caacctgcag tccctggggt tggggtaggg agtccgagag
     7801 cagccaaggg gcaggtttgg tgtcatgggc tcagtcccca gagcgcacgg ggaggggaag
     7861 gttccatggc aggacctctg tatagggact ccggggatga cgactcgggc tggaggccag
     7921 gcaggacgac aggaggtggg gagcaggaga actccgggat ggggaagcag gagccccagg
     7981 gacgggacac tcagggagca gtggccgtct ccagcagcag cagggtcccg gccagcagag
     8041 gaagcaacgc ggggaccacg gaccgccccg ggtgcgcggc gtcggtggtg ccggcggggg
     8101 gcgccaggtc gcaggcggtg tagggctcca ggcaggcggc gaaggccatg acgtgcgcta
     8161 tgaaggtctg ctcctgcacg ccgtgaacca ggtgcgcctg cgggccgcgc gcgaacaccg
     8221 ccacgtcctc gcctgcgtgg gtctcttcgt ccaggggcac tgctgactgc tgccgatact
     8281 cggggctccc gctctcgctc tcggtaacat ccggccgggc gccgtccttg agcacatagc
     8341 ctggaccgtt tccgtatagg aggaccgtgt aggccttcct gtcccgggcc ttgccagggg
     8401 ccagcccgaa gatggagctc cctcgcaggg ggtagcctcc gaaggagaag acgtgggagt
     8461 ggtcggcagt gacgaggctc agcgtgtcct cctcgctggt gagctggccc gccctctcaa
     8521 tggcgtcgtc gaacatgatc gtctcagtca gtgcccggta agccctgctt tcatgatgac
     8581 catggtcgat gcgaccaccc tccacgaaga ggaagaagcc gcgggggttc ctgctcagca
     8641 ggcgcagggc agcctctgtc atctccatca gggaggggtc cagtgtggag tctcggtgga
     8701 tctcgtattt catgtctcca ggctcaaaga gacccatgag atgggtcaca gacgggtcca
     8761 gggaagcctg catgagctca gtgcggttcc acacataccg ggcaccctgg cgcttcgcca
     8821 gccattcctg caccagattc ttcccgtcca gcctggtccc accttggctg tagtcatctg
     8881 ggtactcagg gtctggggtt cccatgcgaa acatgtactt tcggcctcca cctaggatca
     8941 cgtcaatgtc catgttggag atgagctgcg tagcgatgtc ctggcacccc tcctggcggg
     9001 ccgaggcagg cacgtcggcg tccgagtacc agttgcggtt caccgtgtgg gcgtaggtgc
     9061 cggctggcga ggcgtgctgc actcgtgtgg tggttaccac tcccactgac ttccctgctt
     9121 tcttggcccg attcatcacg gagatgacct cgttgccgcg tgtcgtgttg cactggttaa
     9181 agcgggcggc tgcactcaag ccaatggtct ggaagttgcc cttgaccccg cacaggtagg
     9241 ccgtggctgt ggctccactg tctggcacat gtttgtctac attgtatgtc ttggacagag
     9301 ccacatatgg gaagcggtcc atggccaggg gtatctcagg ccccagtttg tccttcttct
     9361 gcccttttag gatcctggca gctgtcaccg tagacacccc catcccatcg cccaggaaga
     9421 tgatgaggtt cttggcggct gtctgtgcag gctgcagctt cttggcgg
//
