LOCUS       sequence-446860-       12256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human lunapark (LNPK) under the
            spleen focus-forming virus (SFFV) promoter and mCherry under the 
            Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12256)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-LNPK-WPRE-UbC-mCherry
FEATURES             Location/Qualifiers
     source          1..12256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2413..2422
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4017..4037)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4435..4446)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5485..5504
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5678..5687
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5684..6394
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5829..5847)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6097..6116
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             6489..6722
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6747..6765)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6748..6765)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7055..7074)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7288..7307)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7407..7429
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7467..7485)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7553..7657
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7658..7726,7727..8518)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7876..7895)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8689..9277
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9178..9197
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9431..9448
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9518..9538)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9523..9852
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9703..9838
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10986..11051
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11181..11201
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11626..11760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11663..11682)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11717..11736
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11928..12256,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag ctgaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 gatccaccgg tcgccaccat gggaaggagt tggcccggct tgtctctctg tcagtggcgg
     2461 ctgctgcctg ctctggaggc aggctgggcg gtggcggccg agactggcgg gggtggacgc
     2521 ccgggccggg ctgcgcccgc ttcttgcagc tgtgaattcc tttggacaat tgatgatatt
     2581 tatcattgtg cccagtttct acaaataaaa gatgggtgga ttattttctc gatggagggt
     2641 aagacaaaac cttcaactgt agaagttcta gaaagtatag ataaggaaat tcaagcattg
     2701 gaagaattta gggaaaaaaa tcagagatta caaaaattat gggttggaag attaattctg
     2761 tattcctcag ttctctatct gtttacatgc ttaattgtat atttgtggta tcttcctgat
     2821 gaatttacag caagacttgc catgacactc ccattttttg cttttccatt gatcatctgg
     2881 agcataagaa cagtaattat tttcttcttt tccaagagaa cagaaagaaa taatgaagca
     2941 ttggatgatt taaaatccca gaggaaaaaa atacttgaag aagtcatgga aaaagaaact
     3001 tacaagacgg ctaaattaat tcttgaaagg tttgatccgg actcaaagaa agcaaaggag
     3061 tgtgagccgc catctgctgg agcagctgta actgcaagac ctggacaaga gattcgtcag
     3121 cgaactgcag ctcaaagaaa cctttctcca acaccagcaa gccctaacca gggccctcct
     3181 ccacaagttc cagtatctcc tggaccacca aaggacagtt ctgcccctgg tggaccccca
     3241 gaaaggactg ttactccagc cctatcatca aatgtgttac caagacatct tggatcccct
     3301 gctacttcag tgcctggaat gggtcttcat cctccaggtc cacctttagc aagacctatt
     3361 ctcccccgag aacgaggtgc tttggataga attgttgaat atttggttgg tgatggtcca
     3421 caaaacaggt atgcacttat atgtcagcag tgtttttctc ataatggcat ggctttgaag
     3481 gaagaatttg aatacattgc ttttcgatgt gcctactgtt ttttcttgaa ccctgcaaga
     3541 aaaaccagac ctcaggctcc aagacttcct gagtttagtt ttgagaagag gcaggtggtg
     3601 gaaggttcaa gttcagttgg tcccttgcca tcaggaagtg tgctttcatc agacaaccag
     3661 tttaatgaag aatctttaga acacgatgtt cttgatgata atacagagca gacagatgac
     3721 aaaataccag ctacagaaca gacaaaccaa gtgattgaaa aagcatctga ctcagaggaa
     3781 ccagaggaga aacaagagac tgagaatgag gaagcctcag tgattgaaac caactccaca
     3841 gttcctggag ctgattctat tcctgatcct gaactaagtg gagaatcttt gacggcagag
     3901 tagagcggcc gcgactctag agtcgacctg caggcatgca agcttgatat caagcttatc
     3961 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4021 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4081 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4141 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4201 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4261 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4321 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4381 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4441 ctcaatccag cggaccttcc ttcccgcggg cgcccccctc ctcacggcga gcgctgccac
     4501 gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc
     4561 ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg
     4621 ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa
     4681 gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt
     4741 atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg
     4801 gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc
     4861 ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag
     4921 ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc
     4981 aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc
     5041 gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct
     5101 aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg
     5161 tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg
     5221 gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga
     5281 cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt
     5341 aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac
     5401 aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt
     5461 tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg
     5521 cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat
     5581 gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt
     5641 tgttagacga agcttggtac cgagctcgga tcaattcgcc accatggtga gcaagggcga
     5701 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     5761 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     5821 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     5881 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     5941 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     6001 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     6061 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     6121 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     6181 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     6241 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     6301 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     6361 ctccaccggc ggcatggacg agctgtacaa gtaactcgag ggaattaatt cgagctcggt
     6421 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6481 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     6541 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6601 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6661 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6721 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
     6781 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     6841 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     6901 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     6961 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     7021 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     7081 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     7141 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     7201 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     7261 ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     7321 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     7381 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     7441 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     7501 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     7561 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7621 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7681 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7741 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7801 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7861 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7921 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7981 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8041 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8101 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8161 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8221 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8281 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8341 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8401 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8461 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8521 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8581 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8641 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8701 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8761 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8821 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8881 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8941 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9001 gggctgaatg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9061 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9121 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9181 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9241 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9301 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9361 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9421 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9481 tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt
     9541 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9601 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9661 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9721 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9781 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9841 ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact
     9901 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
     9961 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
    10021 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
    10081 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
    10141 aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg
    10201 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
    10261 cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
    10321 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
    10381 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
    10441 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
    10501 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
    10561 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
    10621 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
    10681 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
    10741 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct
    10801 cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
    10861 tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga
    10921 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
    10981 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
    11041 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
    11101 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
    11161 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
    11221 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
    11281 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
    11341 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
    11401 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
    11461 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
    11521 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
    11581 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
    11641 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    11701 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    11761 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
    11821 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
    11881 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
    11941 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
    12001 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
    12061 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
    12121 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
    12181 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
    12241 gtacttcaag aactgc
//
