LOCUS       sequence-446861-       11177 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human CKAP4 mutant lacking the 
            luminal domain under the spleen focus-forming virus (SFFV) promoter 
            and Emerald under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11177)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-CKAP4(1-131)-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..11177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(2929..2949)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3347..3358)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4397..4416
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4590..4599
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4596..4598,4599..4601,4602..5315)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4641..4662)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4902..4921)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5249..5270
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5410..5643
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5668..5686)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5669..5686)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5976..5995)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6209..6228)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6328..6350
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6388..6406)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6474..6578
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6579..6647,6648..7439)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6797..6816)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7610..8198
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8099..8118
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8352..8369
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8439..8459)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8444..8773
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8624..8759
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9907..9972
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10102..10122
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10547..10681
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10584..10603)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10638..10657
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10849..11177,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccaccg gtcgccacca tgccctcggc caaacaaagg ggctccaagg gcggccacgg
     2461 cgccgcgagc ccctcggaga agggtgccca cccgtcgggc ggcgcggatg acgtggcgaa
     2521 gaagccgccg ccggcgccgc agcagccgcc gccgccgccc gcgccgcacc cgcagcagca
     2581 cccgcagcag cacccgcaga accaggcgca cggcaagggc ggccaccgcg gcggcggcgg
     2641 cggcggcggc aagtcctcct cctcctcctc cgcctccgcc gccgctgccg ccgccgccgc
     2701 ctcgtcctcg gcgtcctgct cgcgcaggct cggcagggcg ctcaactttc tcttctacct
     2761 cgccctggtg gcggcggccg ctttctcggg ctggtgcgtc caccacgtcc tgtaaagcgg
     2821 ccgcgactct agagtcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca
     2881 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     2941 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3001 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3061 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3121 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3181 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3241 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     3301 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3361 agcggacctt ccttcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg
     3421 aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct
     3481 cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg
     3541 gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc
     3601 ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg
     3661 acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt
     3721 ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt
     3781 cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag
     3841 tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc
     3901 ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac
     3961 aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg
     4021 aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt
     4081 gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc
     4141 gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc
     4201 gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt
     4261 ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg
     4321 gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc
     4381 tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg
     4441 ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt
     4501 cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac
     4561 gaagcttggt accgagctcg gatcaattcg ccaccatggt gagcaagggc gaggagctgt
     4621 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4681 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     4741 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg
     4801 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4861 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4921 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4981 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     5041 acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc
     5101 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     5161 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     5221 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     5281 ggatcactct cggcatggac gagctgtaca agtaactcga gggaattaat tcgagctcgg
     5341 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5401 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     5461 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5521 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5581 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5641 gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat
     5701 acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg
     5761 tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt
     5821 cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt
     5881 cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc
     5941 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     6001 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     6061 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     6121 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     6181 gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     6241 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     6301 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     6361 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     6421 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     6481 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6541 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6601 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6661 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6721 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6781 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6841 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6901 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6961 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7021 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7081 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7141 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7201 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7261 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7321 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7381 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7441 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7501 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7561 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7621 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7681 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7741 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7801 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7861 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7921 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7981 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8041 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8101 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8161 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8221 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8281 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8341 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8401 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     8461 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8521 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8581 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     8641 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8701 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8761 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
     8821 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
     8881 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
     8941 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
     9001 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
     9061 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
     9121 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
     9181 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
     9241 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
     9301 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
     9361 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
     9421 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
     9481 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
     9541 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
     9601 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
     9661 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
     9721 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
     9781 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
     9841 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     9901 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     9961 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
    10021 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
    10081 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
    10141 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
    10201 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
    10261 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
    10321 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
    10381 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
    10441 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
    10501 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    10561 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10621 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    10681 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    10741 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
    10801 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
    10861 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
    10921 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
    10981 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
    11041 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
    11101 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
    11161 agtacttcaa gaactgc
//
