LOCUS       sequence-446863-       14003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human CKAP4 mutant with an 
            additional luminal domain under the spleen focus-forming virus 
            (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14003)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-CKAP4(2xlumen)-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..14003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(5755..5775)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6173..6184)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7223..7242
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      7416..7425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7422..7424,7425..7427,7428..8141)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7467..7488)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7728..7747)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8075..8096
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             8236..8469
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8494..8512)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8495..8512)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8802..8821)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9035..9054)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9154..9176
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9214..9232)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9300..9404
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9405..9473,9474..10265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9623..9642)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10436..11024
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10925..10944
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(11265..11285)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11270..11599
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11450..11585
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12733..12798
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12928..12948
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13373..13507
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13410..13429)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13464..13483
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(13675..14003,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccaccg gtcgccacca tgccctcggc caaacaaagg ggctccaagg gcggccacgg
     2461 cgccgcgagc ccctcggaga agggtgccca cccgtcgggc ggcgcggatg acgtggcgaa
     2521 gaagccgccg ccggcgccgc agcagccgcc gccgccgccc gcgccgcacc cgcagcagca
     2581 cccgcagcag cacccgcaga accaggcgca cggcaagggc ggccaccgcg gcggcggcgg
     2641 cggcggcggc aagtcctcct cctcctcctc cgcctccgcc gccgctgccg ccgccgccgc
     2701 ctcgtcctcg gcgtcctgct cgcgcaggct cggcagggcg ctcaactttc tcttctacct
     2761 cgccctggtg gcggcggccg ctttctcggg ctggtgcgtc caccacgtcc tggaggaggt
     2821 ccagcaggtc cggcgcagcc accaggactt ctcccggcag agggaggagc tgggccaggg
     2881 cttgcagggc gtcgagcaga aggtgcagtc tttgcaagcc acatttggaa cttttgagtc
     2941 catcttgaga agctcccaac ataaacaaga cctcacagag aaagctgtga agcaagggga
     3001 gagtgaggtc agccggatca gcgaagtgct gcagaaactc cagaatgaga ttctcaaaga
     3061 cctctcggat gggatccatg tggtgaagga cgcccgggag cgggacttca cgtccctgga
     3121 gaacacggtg gaggagcggc tgacggagct caccaaatcc atcaacgaca acatcgccat
     3181 cttcacagaa gtccagaaga ggagccagaa ggagatcaat gacatgaagg caaaggttgc
     3241 ctccctggaa gaatctgagg ggaacaagca ggatttgaaa gccttaaagg aagctgtgaa
     3301 ggagatacag acctcagcca agtccagaga gtgggacatg gaggccctga gaagtaccct
     3361 tcagactatg gagtctgaca tctacaccga ggtccgcgag ctggtgagcc tcaagcagga
     3421 gcagcaggct ttcaaggagg cggccgacac ggagcggctc gccctgcagg ccctcacgga
     3481 gaagcttctc aggtctgagg agtccgtctc ccgcctcccg gaggagatcc ggagactgga
     3541 ggaagagctc cgccagctga agtccgattc ccacgggccg aaggaggacg gaggcttcag
     3601 acactcggaa gcctttgagg cactccagca aaagagtcag ggactggact ccaggctcca
     3661 gcacgtggag gatggggtgc tctccatgca ggtggcttct gcgcgccaga ccgagagcct
     3721 ggagtccctc ctgtccaaga gccaggagca cgagcagcgc ctggccgccc tgcaggggcg
     3781 cctggaaggc ctcgggtcct cagaggcaga ccaggatggc ctggccagca cggtgaggag
     3841 cctgggcgag acccagctgg tgctctacgg tgacgtggag gagctgaaga ggagtgtggg
     3901 cgagctcccc agcaccgtgg aatcactcca gaaggtgcag gagcaggtgc acacgctgct
     3961 cagtcaggac caagcccagg ccgcccgtct gcctcctcag gacttcctgg acagactttc
     4021 ttctctagac aacctgaaag cctcagtcag ccaagtggag gcggacttga aaatgctcag
     4081 gactgctgtg gacagtttgg ttgcatactc ggtcaaaata gaaaccaacg agaacaatct
     4141 ggaatcagcc aagggtttac tagatgacct gaggaatgat ctggataggt tgtttgtgaa
     4201 agtggagaag attcacgaaa aggtcgaaga agtacagcag gtgagaaggt cccatcagga
     4261 ctttagcaga cagagggagg agctgggcca gggcctgcag ggcgtggagc agaaagtgca
     4321 gagcctgcag gcgacattcg ggacctttga gtccatcctg cggagcagcc agcacaagca
     4381 ggatctgacc gagaaggccg ttaagcaggg cgaaagcgag gtgtccagaa tctccgaggt
     4441 gctgcagaaa ctgcagaatg agatcctgaa ggatctgagt gacggaatcc acgtggtgaa
     4501 ggacgccagg gagcgcgact tcacctccct ggagaatact gtggaggaga gactgacaga
     4561 gctgaccaag agcattaacg acaatattgc catctttaca gaggtgcaga agcggtccca
     4621 gaaggagatc aacgacatga aggccaaggt ggcctctctg gaggagagcg agggaaacaa
     4681 gcaggatctg aaggccctga aggaggccgt gaaggagatc cagactagcg ccaagagccg
     4741 ggagtgggac atggaggccc tgaggtccac cctgcagacg atggagtccg acatctatac
     4801 cgaggtgcgc gagctggtgt ctctgaagca ggagcagcag gccttcaaag aggccgcaga
     4861 tactgagcgc ctggctctgc aggctctgac cgagaagctg ctccgcagcg aggagtcagt
     4921 gtccagactg cccgaggaga tcaggagact ggaggaggag ctgcggcagc tgaagagcga
     4981 tagccacggc ccaaaggaag atggaggctt ccggcatagt gaggcctttg aggccctgca
     5041 gcagaagtcc caggggctgg acagcagact gcagcacgtg gaagatggcg tgctgtccat
     5101 gcaggtcgcc agcgccaggc agaccgaaag cctggaaagc ctgctgagca agagccagga
     5161 gcacgaacag aggctggccg ccctgcaggg acggctggag ggcctgggaa gcagcgaggc
     5221 cgaccaggat ggactggcct ccaccgtgag atcactgggc gagacccagc tggtgctgta
     5281 tggcgacgtg gaggagctca agcggtcagt gggcgagctc ccttccacag tggagagcct
     5341 gcagaaggtg caggagcagg tgcatacatt acttagccag gaccaggccc aggccgctag
     5401 actgcccccc caggacttcc tggacagact gagctccctg gacaatctga aggccagtgt
     5461 gtcacaagtc gaagccgacc tgaaaatgct gagaacagcc gtggattctc tggtggcata
     5521 ctctgtcaag atcgagacaa acgagaacaa cctggagagc gccaagggcc tgctggacga
     5581 cctgagaaat gacctggata gactcttcgt taaggtggag aagatccatg agaaggtgta
     5641 aagcggccgc gactctagag tcgacctgca ggcatgcaag cttgatatca agcttatcga
     5701 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5761 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5821 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     5881 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     5941 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6001 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6061 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     6121 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6181 caatccagcg gaccttcctt cccgcgggcg cccccctcct cacggcgagc gctgccacgt
     6241 cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc
     6301 gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg
     6361 acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt
     6421 agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat
     6481 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt
     6541 tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg
     6601 ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg
     6661 ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa
     6721 aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt
     6781 tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa
     6841 tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg
     6901 aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc
     6961 ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg
     7021 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag
     7081 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag
     7141 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta
     7201 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg
     7261 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
     7321 aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg
     7381 ttagacgaag cttggtaccg agctcggatc aattcgccac catggtgagc aagggcgagg
     7441 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     7501 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     7561 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttgacct
     7621 acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     7681 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     7741 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     7801 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     7861 acagccacaa ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca
     7921 agacccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     7981 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     8041 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     8101 ccgccgggat cactctcggc atggacgagc tgtacaagta actcgaggga attaattcga
     8161 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8221 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     8281 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8341 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8401 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8461 tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg
     8521 tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc
     8581 taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt
     8641 cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga
     8701 aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat
     8761 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     8821 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     8881 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     8941 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     9001 gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     9061 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     9121 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     9181 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     9241 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     9301 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9361 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     9421 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9481 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9541 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     9601 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     9661 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     9721 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     9781 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     9841 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     9901 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     9961 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10021 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    10081 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    10141 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    10201 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    10261 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    10321 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    10381 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    10441 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    10501 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    10561 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    10621 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    10681 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    10741 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    10801 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    10861 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    10921 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    10981 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    11041 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    11101 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    11161 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    11221 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
    11281 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    11341 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    11401 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
    11461 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    11521 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
    11581 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
    11641 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
    11701 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
    11761 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
    11821 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
    11881 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
    11941 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
    12001 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
    12061 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
    12121 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
    12181 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
    12241 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
    12301 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
    12361 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
    12421 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
    12481 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
    12541 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
    12601 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
    12661 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
    12721 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
    12781 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
    12841 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
    12901 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
    12961 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
    13021 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
    13081 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
    13141 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
    13201 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
    13261 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
    13321 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    13381 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    13441 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    13501 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    13561 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    13621 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
    13681 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
    13741 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
    13801 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
    13861 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
    13921 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
    13981 atccggagta cttcaagaac tgc
//
