LOCUS       sequence-446876-        9532 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Activation tagging for plant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9532)
  TITLE     Plant activation tagging vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKANAC3K
FEATURES             Location/Qualifiers
     source          1..9532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1217..1396
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             1439..2236
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1496..1515)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2106..2125
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2293..2467
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(2636..2655)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2755..2777
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2815..2833)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2901..3005
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3006..3074,3075..3866)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3224..3243)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4037..4625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4526..4545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6670..6686)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6670..6686)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     rep_origin      6815..7009
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7075..8148)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8577..9206)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctcgagcttg gttcgagccc atattctgat caccatagcg ccgaaggcgc tttcacaggt
       61 gacggcctcc cttgggaagg tcacggccgt tatcgctctt cattctgtga gtggcacttt
      121 tgatttgatt gcgattatcg aagccccttc gatttcagag ctcgatcagt tcatcgatca
      181 aatcggcata attgaaggtg tcgagcggac gctctcttcg attattcttt caacacgcat
      241 ttcacgctga aagagcaaga ggccacagcc gtgcgcctgc tgccaccttt ccgacgatga
      301 ccatgcagaa cttacggcct ctatgtcggt gtattccacc caccgacgcg ccatttccgt
      361 gtgcattaag ataatgcttt ccgccccatc ggattgctgt tctgacatgg tatatcatta
      421 cgacagccaa tgtttggcgg ctcgtacgaa tcatcaagcg gtcaacctgt gctgtcaacc
      481 gtcacgatat gagcaagcaa ctcaaacgct ttcctattca ccgcacgcgc gttgttggct
      541 gtttggcagg atatatgcca acgtaaaaat gagggcaatc gattgtactg aatcggattt
      601 tcaagggtct ggccaaaact attccgtggg cacctggcac acgccctgga gtccggcccg
      661 tttccagttg agggttgtct acgcttagat gagaaggaaa gttgtccaag acgaatccca
      721 gtgtcctatt accaatagcc gacggtatcg ataagctgct cgagactagt gagctcatgc
      781 atgcggccgc gtcgacaagc ttatcgacgg atcagcttca cgctgccgca agcactcagg
      841 gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg
      901 accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag
      961 aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac
     1021 agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa
     1081 agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat caagatcatg
     1141 agcggagaat taagggagtc acgttatgac ccccgccgat gacgcgggac aagccgtttt
     1201 acgtttggaa ctgacagaac cgcaacgttg aaggagccac tcagccgcgg gtttctggag
     1261 tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg
     1321 tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc cataaattcc
     1381 cctcggtatc caattagagt ctcatattca ctctcaatcc agatctgatc ccgggaccat
     1441 ggggattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     1501 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt ttcggctgtc
     1561 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     1621 ccaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     1681 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     1741 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     1801 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     1861 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     1921 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     1981 cggcgaggat ctcgtcgtga cacatggcga tgcctgcttg ccgaatatca tggtggaaaa
     2041 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     2101 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     2161 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     2221 tgacgagttc ttctgagcgg gactctgggg ttcggatcga tcctctagct agagtcgatc
     2281 gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg gaattagggt
     2341 tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
     2401 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat
     2461 ccagatcccc cgaattaatt ggtaccgaag ggcgaatttg gcgtaatagc gaagaggccc
     2521 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     2581 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     2641 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     2701 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     2761 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     2821 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     2881 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2941 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3001 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3061 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3121 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3181 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3241 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3301 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3361 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3421 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3481 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     3541 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3601 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3661 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3721 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3781 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3841 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3901 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3961 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     4201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4681 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4741 gagctggatc cccaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga
     4801 tacagtctca gaagaccaga gggctattga gacttttcaa caaagggtaa tatcgggaaa
     4861 cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga
     4921 agatggcttc tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc
     4981 taccgacagt ggtcccaaag atggaccccc acccacgagg aacatcgtgg aaaaagaaga
     5041 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctagatcc ccaacatggt
     5101 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
     5161 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     5221 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
     5281 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
     5341 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     5401 gcaagtggat tgatgtgata tctagatccc caacatggtg gagcacgaca ctctcgtcta
     5461 ctccaagaat atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca
     5521 aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga
     5581 aaggacagta gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc
     5641 tatcgttcaa gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa
     5701 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
     5761 ctagatcccc aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac
     5821 agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
     5881 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaaga
     5941 tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctac
     6001 cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa aagaagacgt
     6061 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tagatccgaa actatcagtg
     6121 tttgacagaa gggcgaattc gcccttcccc aagtgcagcg tttaataaat cgtgtgacag
     6181 gatatatctt gtggtcaggt agataaattt gtgtggaggt atgtttgttt aggacggcgg
     6241 tgatgatggc ttgcgcctta cgtcccgaaa gcagtccacg aagacaattg caatatcggc
     6301 tgtcttccat tcccttcgtg gcatcttgtc aacgcgcctc gggagctcag tcgccgacgt
     6361 ttcgcgagtt cggtatgcaa ccccgacctt gcgaagaacc gcttacggaa ccgcgctcta
     6421 tgagaaccgc cgggagaaga gtttccttgg gtggggctga agtctcgccc tttaagcggg
     6481 cgatcaggtc ttctgtggca gccgtcgcga gatccctgac aggttgcgaa atcacggtca
     6541 atggcggcgt tatagcggat gtccagtcgg cgtcgtcaaa gcagataaga gagacatcat
     6601 cgggaatcga aagatccatt tcccgaagaa ctccaaggag aacgagggct acggagctgt
     6661 ctgtgggggg atccactagt tctagagtag gccagccatt tttgagcggc cagcggccgc
     6721 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     6781 ttgtagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     6841 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     6901 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     6961 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     7021 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     7081 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     7141 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     7201 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     7261 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     7321 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     7381 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     7441 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     7501 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     7561 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     7621 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
     7681 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
     7741 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
     7801 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
     7861 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
     7921 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
     7981 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
     8041 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
     8101 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
     8161 ggcttggggg ttccagtgcc attgcagggc cggcaggcaa cccagccgct tacgcctggc
     8221 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
     8281 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
     8341 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
     8401 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
     8461 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
     8521 acggccggca cttagcgtgt ttgtgctttt gctcatcttc tctttacctc attaactcaa
     8581 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
     8641 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
     8701 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
     8761 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
     8821 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
     8881 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
     8941 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
     9001 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
     9061 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
     9121 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
     9181 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
     9241 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
     9301 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
     9361 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
     9421 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
     9481 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tc
//
