LOCUS       sequence-446878-        5291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0780m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in striatal D1 MSNs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Hunker AC, Wirthlin ME, Gill G, Johansen NJ, Hooper M, Omstead V, 
            Vargas S, Lerma MN, Taskin N, Weed N, Laird WD, Bishaw YM, Bendrick 
            JL, Gore BB, Ben-Simon Y, Opitz-Araya X, Martinez RA, Way SW, 
            Thyagarajan B, Otto S, Sanchez REA, Alexander JR, Amaya A, Amster A,
            Arbuckle J, Ayala A, Baker PM, Barcelli T, Barta S, Bertagnolli D, 
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chong P, Clark M, Colbert K, 
            Daniel S, Dawe T, Departee M, DiValentin P, Donadio NP, Dotson NI, 
            Dwivedi D, Egdorf T, Fliss T, Gary A, Goldy J, Grasso C, Groce EL, 
            Gudsnuk K, Han W, Haradon Z, Hastings S, Helback O, Ho WV, Huang C, 
            Johnson T, Jones DL, Juneau Z, Kenney J, Leibly M, Li S, Liang E, 
            Loeffler H, Lusk NA, Madigan Z, Malloy J, Malone J, McCue R, Melchor
            J, Mich JK, Moosman S, Morin E, Naidoo R, Newman D, Ngo K, Nguyen K,
            Oster AL, Ouellette B, Oyama AA, Pena N, Pham T, Phillips E, Pom C, 
            Potekhina L, Ransford S, Ray PL, Reding M, Rette DF, Reynoldson C, 
            Rimorin C, Sigler AR, Rocha DB, Ronellenfitch K, Ruiz A, Sawyer L, 
            Sevigny JP, Shapovalova NV, Shepard N, Shulga L, Soliman S, Staats 
            B, Taormina MJ, Tieu M, Wang Y, Wilkes J, Wood T, Zhou T, Williford 
            A, Dee N, Mollenkopf T, Ng L, Esposito L, Kalmbach BE, Yao S, Ariza 
            J, Collman F, Mufti S, Smith K, Waters J, Ersing I, Patrick M, Zeng 
            H, Lein ES, Kojima Y, Horwitz G, Owen SF, Levi BP, Daigle TL, Tasic 
            B, Bakken TE, Ting JT
  TITLE     Enhancer AAV toolbox for accessing and perturbing striatal cell 
            types and circuits.
  JOURNAL   Neuron. 2025 May 21;113(10):1507-1524.e17. doi: 
            10.1016/j.neuron.2025.04.035.
  PUBMED    40403704
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.04"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1507-1524.e17"
COMMENT     Sequence Label: AiP15581 - pAAV-AiE0780m-minBG-jGCaMP8s-WPRE3-...
FEATURES             Location/Qualifiers
     source          1..5291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     RBS             364..372
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             769..786
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     1068..1089
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1195..1216)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1456..1475)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(2118..2138)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    2325..2436
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2554..2694
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2554..2683
                     /label=AAV2 ITR
     rep_origin      2769..3224
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2856..2875)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3066..3087
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3241..3260)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3360..3382
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3420..3438)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3506..3610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3611..3679,3680..4471)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3829..3848)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4642..5230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5131..5150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtttc acaaactaca ggttcactgg agcacagcca
      181 tgccaacatg gcatctaagg ctggtatgac ctcgagaccc ctccagcact ccttcgttca
      241 gccatagaac aaggagacac agataaagtc tggagccggg cccaggtacc tgaggctcag
      301 agatgagccc gcagcgaagc tgacaactga ctcattcctc cgacactctc ccaccctccc
      361 agctaaggag gtgtctgatg tgaacatgtt ctgcctcttt gttgcctgct gttgggggcg
      421 agggctttgg gtggattttg aagagcaatt atgcttatta ctggttagga tttctctgtt
      481 tgctgagtgc attaactttt agcgcttccc atcactcatt cttcttcaca ccctcttgtc
      541 agttacaaat gtgcaggtgg agagccacga aaaaaccctg cagcaacggc aacagaaatc
      601 acagcaacaa caacaaatga acagaaagga tagaaagcag atactcaaag atggagaaaa
      661 agatactccc gagaggctga gctcgggctg ggcataaaag tcagggcaga gccatctatt
      721 gcttacattt gcttctggga tccagatctt tcgaagctag ccaccatgca tcatcaccat
      781 catcacacgc gtcgcaagaa gaccttcaag gaggtggcca ccgccgtgaa gatcatcgcc
      841 atgctgatgg gcctgaagat caacgtctat atcaaggccg acaagcagaa gaacggcatc
      901 aaggcgaact tccacatccg ccacaacatc gaggacggcg gcgtgcagct cgcctaccac
      961 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1021 agcgtggagt ccaaactttc gaaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1081 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc
     1141 ggagggagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1201 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg tgagggcgat
     1261 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1321 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1381 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacat ccaggagcgc
     1441 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1501 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1561 ctggggcaca agctggagta caacctgccg gaccaactga ctgaagagca gatcgcagaa
     1621 tacaaagagg ctttctccct atttgacaag gacggggatg ggacaataac aaccaaggag
     1681 ctggggacgg tgatgcggtc tctcggacac aaccccacag aagcagagct gcaggacatg
     1741 atcaatgaag tagatgccga cggtgacggc acaatcgact tccctgagtt cctgacaatg
     1801 atggcaagaa aaatgaaata cagggacacg gaagaagaaa ttagagaagc gttcggtgtg
     1861 tttgataagg atggcaatgg ctacatcagt gcagcagagc ttcgccacgt gatgacaaac
     1921 cttggagaga agttaacaga tgaagaggtt gatgaaatga tcagggaagc agacatcgat
     1981 ggggatggtc aggtaaacta cgaagagttt gtacaaatga tgacagcgaa gtaaggcgcg
     2041 ccgcggccgc gaattcgata tcataatcaa cctctggatt acaaaatttg tgaaagattg
     2101 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2161 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2221 ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2281 cggctgttgg gcactgacaa ttccgtggct cgagagatct tcgactgtgc cttctagttg
     2341 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     2401 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     2461 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     2521 gcatgagatc tcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     2581 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     2641 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     2701 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     2761 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     2821 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
     2881 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     2941 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     3001 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3061 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
     3121 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
     3181 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     3241 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     3301 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     3361 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     3421 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     3481 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3541 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3601 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3661 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3721 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3781 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3841 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3901 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3961 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4021 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4081 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4141 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4201 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4261 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4321 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4381 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4441 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4501 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4561 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4621 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4681 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4741 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4801 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4861 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4921 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4981 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5041 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5101 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5161 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5221 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5281 tgctcacatg t
//
