LOCUS       sequence-446882-       10955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant binary vector expressing GFP by a enhanced 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10955)
  TITLE     Binary plant vector expressing GFP driven by enhanced 35S promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKAN43Ag8-GFP
FEATURES             Location/Qualifiers
     source          1..10955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1173..1352
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             1470..2267
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1527..1546)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2137..2156
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2324..2498
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        3990..4036
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             join(4134..4136,4137..4139,4140..4853)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MHSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4179..4200)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4440..4459)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4787..4808
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4864..5112
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        6314..6418
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6419..6487,6488..7279)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6637..6656)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7450..8038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7939..7958
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      8236..8430
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8496..9569)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 ctcgagcttg gttcgagccc atattctgat caccatagcg ccgaaggcgc tttcacaggt
       61 gacggcctcc cttgggaagg tcacggccgt tatcgctctt cattctgtga gtggcacttt
      121 tgatttgatt gcgattatcg aagccccttc gatttcagag ctcgatcagt tcatcgatca
      181 aatcggcata attgaaggtg tcgagcggac gctctcttcg attattcttt caacacgcat
      241 ttcacgctga aagagcaaga ggccacagcc gtgcgcctgc tgccaccttt ccgacgatga
      301 ccatgcagaa cttacggcct ctatgtcggt gtattccacc caccgacgcg ccatttccgt
      361 gtgcattaag ataatgcttt ccgccccatc ggattgctgt tctgacatgg tatatcatta
      421 cgacagccaa tgtttggcgg ctcgtacgaa tcatcaagcg gtcaacctgt gctgtcaacc
      481 gtcacgatat gagcaagcaa ctcaaacgct ttcctattca ccgcacgcgc gttgttggct
      541 gtttggcagg atatatgcca acgtaaaaat gagggcaatc gattgtactg aatcggattt
      601 tcaagggtct ggccaaaact attccgtggg cacctggcac acgccctgga gtccggcccg
      661 tttccagttg agggttgtct acgcttagat gagaaggaaa gttgtccaag acgaatccca
      721 gtgtcctatt accaatagcc gacggtatcg ataagcttat cgacggatca gcttcacgct
      781 gccgcaagca ctcagggcgc aagggctgct aaaggaagcg gaacacgtag aaagccagtc
      841 cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa
      901 acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact
      961 gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg
     1021 ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca
     1081 ggggatcaag atcatgagcg gagaattaag ggagtcacgt tatgaccccc gccgatgacg
     1141 cgggacaagc cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactcag
     1201 ccgcgggttt ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt
     1261 caaaagtcgc ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg
     1321 acgttccata aattcccctc ggtatccaat tagagtctca tattcactct caatccagat
     1381 ctgatcccat catcacaaca caaatcaaaa caagaataac aaaatctttc tcttataaat
     1441 tcttatttca agacatcaaa ggagaaacca tggggattga acaagatgga ttgcacgcag
     1501 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     1561 gctgctctga tgccgccgtg tttcggctgt cagcgcaggg gcgcccggtt ctttttgtca
     1621 agaccgacct gtccggtgcc ctgaatgaac tccaggacga ggcagcgcgg ctatcgtggc
     1681 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     1741 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     1801 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     1861 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     1921 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     1981 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acacatggcg
     2041 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     2101 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     2161 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     2221 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     2281 gttcggatcg atcctctagc tagagtcgat cgacaagctc gagtttctcc ataataatgt
     2341 gtgagtagtt cccagataag ggaattaggg ttcctatagg gtttcgctca tgtgttgagc
     2401 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
     2461 aattcctaaa accaaaatcc agtactaaaa tccagatccc ccgaattaat tggtaccggc
     2521 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2581 cctttgagtg agctggatcc ccaacatggt ggagcacgac actctcgtct actccaagaa
     2641 tatcaaagat acagtctcag aagaccagag ggctattgag acttttcaac aaagggtaat
     2701 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg aaaggacagt
     2761 agaaaaggaa gatggcttct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
     2821 agatgcctct accgacagtg gtcccaaaga tggaccccca cccacgagga acatcgtgga
     2881 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctagatccc
     2941 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
     3001 agaccagagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
     3061 ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag atggcttcta
     3121 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctcta ccgacagtgg
     3181 tcccaaagat ggacccccac ccacgaggaa catcgtggaa aaagaagacg ttccaaccac
     3241 gtcttcaaag caagtggatt gatgtgatat ctagatcccc aacatggtgg agcacgacac
     3301 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccagaggg ctattgagac
     3361 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
     3421 cttcatcgaa aggacagtag aaaaggaaga tggcttctac aaatgccatc attgcgataa
     3481 aggaaaggct atcgttcaag atgcctctac cgacagtggt cccaaagatg gacccccacc
     3541 cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     3601 atgtgatatc tagatcccca acatggtgga gcacgacact ctcgtctact ccaagaatat
     3661 caaagataca gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc
     3721 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     3781 aaaggaagat ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
     3841 tgcctctacc gacagtggtc ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa
     3901 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct agatccgaaa
     3961 ctatcagtgt ttgacagaag ggcgaattcg caagaccctt cctctatata aggaagttca
     4021 tttcatttgg agagaacacg ggggactcta gaatcatcac aacacaaatc aaaacaagaa
     4081 taacaaaatc tttctcttat aaattcttat ttcaagacat caaaggagaa aagatgcata
     4141 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     4201 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4261 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4321 ccaccttcac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     4381 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4441 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4501 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4561 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4621 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4681 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4741 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4801 agttcgtgac cgccgccggg atcactcacg gcatggacga gctgtacaag taaggcgcgc
     4861 caatatgaag atgaagatga aatatttggt gtgtcaaata aaaagcttgt gtgcttaagt
     4921 ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg ctttttctaa tattaaatga
     4981 atgtaagatc tcattataat gaataaacaa atgtttctat aatccattgt gaatgttttg
     5041 ttggatctct tctgcagcat ataactactg tatgtgctat ggtatggact atggaatatg
     5101 attaaagata agatgggctc atagagtaaa acgaggcgag ggacctataa acctcccttc
     5161 atcatgctat ttcatgatct attttataaa ataaagatgt agaaaaaagt aagcgtaata
     5221 accgcaaaac aaatgattta aaacatggca cataatgagg agattaagtt cggtttacgt
     5281 ttattttagt actaattgta acgtgagact acgtatcggg aatcgcctaa ttaaagcatt
     5341 aatgcgaacc tgattagatt caccgaccct cctatcgtgt cgacctttct gtttcttaga
     5401 attttttggt agtctatgta ctaataatgt cagcttcgta tttatttcat aagcaatttg
     5461 catttgcaat ttgtttttta cttttatttt tattgtattg tggaatgtgg actcgtacca
     5521 acatgaagtt atataccacc aaaaaaatta cagttagtca aaagattcac gagtgagagc
     5581 tacttatgat tgtcttttac gtatatgtct aattgtctat ttgctcaata atctttgtac
     5641 tttcttttgt cgttgataaa atcacaaagt tccaaaagta atcgaatgat ttgcttttaa
     5701 gaaaagaaga gctcaataat tcaacatata tctgtacaca gtttaaacca attcgccctt
     5761 ccccaagtgc agcgtttaat aaatcgtgtg acaggatata tcttgtggtc aggtagataa
     5821 atttgtgtgg aggtatgttt gtttaggacg gcggtgatga tggcttgcgc cttacgtccc
     5881 gaaagcagtc cacgaagaca attgcaatat cggctgtctt ccattccctt cgtggcatct
     5941 tgtcaacgcg cctcgggagc tcagtcgccg acgtttcgcg agttcggtat gcaaccccga
     6001 ccttgcgaag aaccgcttac ggaaccgcgc tctatgagaa ccgccgggag aagagtttcc
     6061 ttgggtgggg ctgaagtctc gccctttaag cgggcgatca ggtcttctgt ggcagccgtc
     6121 gcgagatccc tgacaggttg cgaaatcacg gtcaatggcg gcgttatagc ggatgtccag
     6181 tcggcgtcgt caaagcagat aagagagaca tcatcgggaa tcgaaagatc catttcccga
     6241 agaactccaa ggagaacgag ggctacggag ctgtctgtgg gggacgtcag gtggcacttt
     6301 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6361 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6421 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6481 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6541 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6601 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6661 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6721 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6781 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6841 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6901 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6961 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7021 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7081 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7141 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7201 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7261 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7321 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7381 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7441 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7501 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7561 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7621 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7681 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7741 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7801 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7861 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7921 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7981 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8041 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtg
     8101 tgaccgggta ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg
     8161 gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgtagctc ttcggctgtg
     8221 cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa
     8281 gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac
     8341 cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc
     8401 tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct
     8461 gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc
     8521 tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc
     8581 aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc
     8641 ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct
     8701 gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc
     8761 aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg
     8821 tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg
     8881 atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg
     8941 gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc
     9001 aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg
     9061 tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat
     9121 tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg
     9181 gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca
     9241 gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg
     9301 cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc
     9361 ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga
     9421 tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg
     9481 gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt
     9541 accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc
     9601 cattgcaggg ccggcaggca acccagccgc ttacgcctgg ccaaccgccc gttcctccac
     9661 acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt
     9721 tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg
     9781 atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc
     9841 ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc
     9901 aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg
     9961 tttgtgcttt tgctcatctt ctctttacct cattaactca aatgagtttt gatttaattt
    10021 cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg
    10081 ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa
    10141 tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga
    10201 tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct
    10261 taaccagctc caccaggtcg gcggtggccc atatatgtcg taagggcttg gctgcaccgg
    10321 aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc
    10381 tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg
    10441 gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc
    10501 actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg
    10561 ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat
    10621 aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg
    10681 accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg
    10741 gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc
    10801 aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc
    10861 gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg
    10921 tgcggtttca tgcttgttcc tcttggcgtt cattc
//
