LOCUS       sequence-446899-        5416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0367h is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in striatal MSNs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Hunker AC, Wirthlin ME, Gill G, Johansen NJ, Hooper M, Omstead V, 
            Vargas S, Lerma MN, Taskin N, Weed N, Laird WD, Bishaw YM, Bendrick 
            JL, Gore BB, Ben-Simon Y, Opitz-Araya X, Martinez RA, Way SW, 
            Thyagarajan B, Otto S, Sanchez REA, Alexander JR, Amaya A, Amster A,
            Arbuckle J, Ayala A, Baker PM, Barcelli T, Barta S, Bertagnolli D, 
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chong P, Clark M, Colbert K, 
            Daniel S, Dawe T, Departee M, DiValentin P, Donadio NP, Dotson NI, 
            Dwivedi D, Egdorf T, Fliss T, Gary A, Goldy J, Grasso C, Groce EL, 
            Gudsnuk K, Han W, Haradon Z, Hastings S, Helback O, Ho WV, Huang C, 
            Johnson T, Jones DL, Juneau Z, Kenney J, Leibly M, Li S, Liang E, 
            Loeffler H, Lusk NA, Madigan Z, Malloy J, Malone J, McCue R, Melchor
            J, Mich JK, Moosman S, Morin E, Naidoo R, Newman D, Ngo K, Nguyen K,
            Oster AL, Ouellette B, Oyama AA, Pena N, Pham T, Phillips E, Pom C, 
            Potekhina L, Ransford S, Ray PL, Reding M, Rette DF, Reynoldson C, 
            Rimorin C, Sigler AR, Rocha DB, Ronellenfitch K, Ruiz A, Sawyer L, 
            Sevigny JP, Shapovalova NV, Shepard N, Shulga L, Soliman S, Staats 
            B, Taormina MJ, Tieu M, Wang Y, Wilkes J, Wood T, Zhou T, Williford 
            A, Dee N, Mollenkopf T, Ng L, Esposito L, Kalmbach BE, Yao S, Ariza 
            J, Collman F, Mufti S, Smith K, Waters J, Ersing I, Patrick M, Zeng 
            H, Lein ES, Kojima Y, Horwitz G, Owen SF, Levi BP, Daigle TL, Tasic 
            B, Bakken TE, Ting JT
  TITLE     Enhancer AAV toolbox for accessing and perturbing striatal cell 
            types and circuits.
  JOURNAL   Neuron. 2025 May 21;113(10):1507-1524.e17. doi: 
            10.1016/j.neuron.2025.04.035.
  PUBMED    40403704
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.04"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1507-1524.e17"
COMMENT     Sequence Label: AiP12205 -
            pAAV-3xSP10ins-AiE0367h-minRho-FlpO...
FEATURES             Location/Qualifiers
     source          1..5416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      849..858
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(855..860,861..881,882..2153)
                     /codon_start=1
                     /product="nuclear-targeted site-specific recombinase"
                     /label=FLPo
                     /note="FLPo is a mouse codon-optimized fusion between the 
                     SV40 nuclear localization signal and a thermostable version
                     of the Saccharomyces cerevisiae site-specific recombinase 
                     FLP.  Recombination occurs at FRT sequences."
                     /translation="MAPKKKRKVMSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAA
                     ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK
                     LIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESI
                     WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV
                     IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY
                     QLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS
                     DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
                     KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
     primer_bind     complement(2243..2263)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    2450..2561
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2679..2819
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2679..2808
                     /label=AAV2 ITR
     rep_origin      2894..3349
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2981..3000)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3191..3212
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3366..3385)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3485..3507
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3545..3563)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3631..3735
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3736..3804,3805..4596)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3954..3973)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4767..5355
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5256..5275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgccggtac cgaagctacc cctaacacac tattctacac
      181 acagaaaatg ctcttcacta ggaagctacc cctaacacac tattctacac acagaaaatg
      241 ctcttcacta ggaagctacc cctaacacac tattctacac acagaaaatg ctcttcacta
      301 gacgcgttag caggagtaca tcctcctctg ttagaaaaac aactctgcgg ttctgggtca
      361 cttttcccat ctttgtatag aaaagtaccg attattattg ttcccccggg tgccttctcc
      421 agcttaaagg aaaattccag ccccagagat cttccgcagt gagtgacagc tccttaatgt
      481 gaggagcagc gtgggcgtgg tgggtgggat gtcagagcca tcaaagccct catttctgat
      541 ccctctgtga tgtcggggtt ccctggcacg cccactgtca tgcctcagca cattggcatc
      601 tgagagagga aaggtgcagc tgacaccctg acccccttct ccccgacacg gggtcagaca
      661 gggtcccgcg gtggcctggt tgtgcagcac tgacatgttg cctgggaacg aagagctcga
      721 ttcagccggg agcttaggga ggggaggtca cttcataagg gcttgggggg ggagttggag
      781 ccacgagtcg tccagccgga gccccgtgtg gctgtgctcc ggcctcagaa gcatccccgg
      841 atccgctagc caccatggct cctaagaaga agaggaaggt gatgagccag ttcgacatcc
      901 tgtgcaagac cccccccaag gtgctggtgc ggcagttcgt ggagagattc gagaggccca
      961 gcggcgagaa gatcgccagc tgtgccgccg agctgaccta cctgtgctgg atgatcaccc
     1021 acaacggcac cgccatcaag agggccacct tcatgagcta caacaccatc atcagcaaca
     1081 gcctgagctt cgacatcgtg aacaagagcc tgcagttcaa gtacaagacc cagaaggcca
     1141 ccatcctgga ggccagcctg aagaagctga tccccgcctg ggagttcacc atcatccctt
     1201 acaacggcca gaagcaccag agcgacatca ccgacatcgt gtccagcctg cagctgcagt
     1261 tcgagagcag cgaggaggcc gacaagggca acagccacag caagaagatg ctgaaggccc
     1321 tgctgtccga gggcgagagc atctgggaga tcaccgagaa gatcctgaac agcttcgagt
     1381 acaccagcag gttcaccaag accaagaccc tgtaccagtt cctgttcctg gccacattca
     1441 tcaactgcgg caggttcagc gacatcaaga acgtggaccc caagagcttc aagctggtgc
     1501 agaacaagta cctgggcgtg atcattcagt gcctggtgac cgagaccaag acaagcgtgt
     1561 ccaggcacat ctactttttc agcgccagag gcaggatcga ccccctggtg tacctggacg
     1621 agttcctgag gaacagcgag cccgtgctga agagagtgaa caggaccggc aacagcagca
     1681 gcaacaagca ggagtaccag ctgctgaagg acaacctggt gcgcagctac aacaaggccc
     1741 tgaagaagaa cgccccctac cccatcttcg ctatcaagaa cggccctaag agccacatcg
     1801 gcaggcacct gatgaccagc tttctgagca tgaagggcct gaccgagctg acaaacgtgg
     1861 tgggcaactg gagcgacaag agggcctccg ccgtggccag gaccacctac acccaccaga
     1921 tcaccgccat ccccgaccac tacttcgccc tggtgtccag gtactacgcc tacgacccca
     1981 tcagcaagga gatgatcgcc ctgaaggacg agaccaaccc catcgaggag tggcagcaca
     2041 tcgagcagct gaagggcagc gccgagggca gcatcagata ccccgcctgg aacggcatca
     2101 tcagccagga ggtgctggac tacctgagca gctacatcaa caggcggatc tgagtcgacg
     2161 gcgcgccgcg gccgcgaatt cgatatcata atcaacctct ggattacaaa atttgtgaaa
     2221 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     2281 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     2341 cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2401 gggctcggct gttgggcact gacaattccg tggctcgaga gatcttcgac tgtgccttct
     2461 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2521 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2581 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     2641 agcaggcatg agatctcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
     2701 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     2761 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     2821 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     2881 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     2941 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     3001 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3061 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     3121 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3181 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
     3241 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
     3301 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     3361 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     3421 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3481 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     3541 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     3601 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3661 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3721 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3781 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3841 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3901 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3961 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4021 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4081 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4141 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4201 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4261 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4321 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4381 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4441 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4501 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4561 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4621 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4681 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4741 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4801 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4861 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4921 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4981 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5041 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5101 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5161 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5221 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5281 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5341 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5401 ccttttgctc acatgt
//
