LOCUS       sequence-446917-        2321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This is the promoter region taken from upstream of Himar transposase
            in pMarC9-Kan. It can be considered the ""default"" promoter to 
            use..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2321)
  TITLE     Cultivarium plasmids
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGLU_85
FEATURES             Location/Qualifiers
     source          1..2321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(365..381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(365..381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(378..400)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(553..1141)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(633..652)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1274..1368)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1389..2048)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1963..1982
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2049..2151)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2238..2260
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2252..2269
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2253..2269
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtatcgcagg tctcaccata tgtttcacaa taacaaaagt tgcttcacaa aagacgctct
       61 atctcacaaa ctaattgact tacagacgtc aaattttgac acgaatcatt tgaaggttgg
      121 tactatataa aaataatatg catttaatac tagcgacgcc atctatgtgt catccatcac
      181 actggcggcc gctaacaggt tggatgataa gtccccggtc tagggtcacg cgtaggacgc
      241 gtcgagtagg gtgcgaggca gggctaaaat agcgtatgag accgtcaggc acgatcgagc
      301 atgctagaag gctaggtgga ggctcagtga tgataagtct gcgatggtgg atgcatgtgt
      361 catggtcata gctgtttcct gtgtgaaatt gttatccgct cagagggcac aatcctattc
      421 cgcgctatcc gacaatctcc aagacattag gtggagttca gttcggcgta tggcatatgt
      481 cgctggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      541 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      601 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      661 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      721 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      781 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      841 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      901 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      961 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1021 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1081 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1141 agaagatcct ttgatctttt ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc
     1201 gtcaagtcag cgtaaatggg tagggggctt caaatcgtcc ccccatacga tataagttgt
     1261 tactagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
     1321 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
     1381 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     1441 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     1501 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     1561 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     1621 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     1681 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     1741 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     1801 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     1861 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     1921 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     1981 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     2041 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     2101 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     2161 tgatacctga aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc
     2221 acaagcgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc
     2281 cgcacgcatc tggaataagg aagtgccatt ccgcctgacc t
//
