LOCUS       sequence-44692-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, 
            Lee TS
  TITLE     Identification and microbial production of a terpene-based advanced 
            biofuel.
  JOURNAL   Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
  PUBMED    21952217
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-09-27"; volume: "2:483. doi"; pages: " 
            10.1038/ncomms1494"
COMMENT     Sequence Label: pBbA5c-MevT-MBIS
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(762..773)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgtgtggaa ttgtgagcgg ataacaattt cagaattcaa aagatcttag gaggaatata
       61 aaatgaaaaa ttgtgtcatc gtcagtgcgg tacgtactgc tatcggtagt tttaacggtt
      121 cactcgcttc caccagcgcc atcgacctgg gggcgacagt aattaaagcc gccattgaac
      181 gtgcaaaaat cgattcacaa cacgttgatg aagtgattat gggtaacgtg ttacaagccg
      241 ggctggggca aaatccggcg cgtcaggcac tgttaaaaag cgggctggca gaaacggtgt
      301 gcggattcac ggtcaataaa gtatgtggtt cgggtcttaa aagtgtggcg cttgccgccc
      361 aggccattca ggcaggtcag gcgcagagca ttgtggcggg gggtatggaa aatatgagtt
      421 tagcccccta cttactcgat gcaaaagcac gctctggtta tcgtcttgga gacggacagg
      481 tttatgacgt aatcctgcgc gatggcctga tgtgcgccac ccatggttat catatgggga
      541 ttaccgccga aaacgtggct aaagagtacg gaattacccg tgaaatgcag gatgaactgg
      601 cgctacattc acagcgtaaa gcggcagccg caattgagtc cggtgctttt acagccgaaa
      661 tcgtcccggt aaatgttgtc actcgaaaga aaaccttcgt cttcagtcaa gacgagttcc
      721 cgaaagcgaa ctcaacggct gaagcgttag gtgcattgcg cccggccttc gataaagcag
      781 gaacagtcac cgctgggaac gcgtctggta ttaacgacgg tgctgccgct ctggtgatta
      841 tggaagaatc tgcggcgctg gcagcaggcc ttacccc
//
