LOCUS       sequence-44694-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Richards CI, Srinivasan R, Xiao C, Mackey ED, Miwa JM, Lester HA
  TITLE     Trafficking of {alpha}4* Nicotinic Receptors Revealed by 
            Superecliptic Phluorin: EFFECTS OF A {beta}4 AMYOTROPHIC LATERAL 
            SCLEROSIS-ASSOCIATED MUTATION AND CHRONIC EXPOSURE TO NICOTINE.
  JOURNAL   J Biol Chem. 2011 Sep 9;286(36):31241-9. Epub 2011 Jul 18.
  PUBMED    21768117
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-09-9"; volume: "286(36)"; pages: "31241-9. Epub 
            2011 Jul 18"
COMMENT     Sequence Label: pEGFP-Sec24D
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      170..758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     659..678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatttatcgc ggcctagagc aagacgtttc ccgttgaata tggctcataa caccccttgt
       61 attactgttt atgtaagcag acagttttat tgttcatgac caaaatccct taacgtgagt
      121 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
      181 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      241 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      301 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      361 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
      421 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      481 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
      541 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
      601 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
      661 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
      721 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      781 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      841 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cg
//
