LOCUS       sequence-446952-        9682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Honnell V, Sweeney S, Norrie J, Parks M, Ramirez C, Jannu AJ, Xu B, 
            Teubner B, Lee AY, Bell C, Dyer MA
  TITLE     Evolutionary conservation of VSX2 super-enhancer modules in retinal 
            development.
  JOURNAL   Development. 2024 Jul 1;151(13):dev202435. doi: 10.1242/dev.202435. 
            Epub 2024 Jul 12.
  PUBMED    38994775
REFERENCE   2  (bases 1 to 9682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2024-07-1"; volume: "151(13):dev202435. doi";
            pages: " 10.1242/dev.202435. Epub 2024 Jul 12"
COMMENT     Sequence Label: Vsx2 mR1-28 +SV40 base prom-GFP-IRES-AP
FEATURES             Location/Qualifiers
     source          1..9682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2353..2457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2458..2526,2527..3318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2676..2695)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3489..4077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3978..3997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4231..4248
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    4316..4450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4340..4359)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4394..4413
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(4456..4651)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4470..4605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    4710..4731
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4746..4751,4752..4769,4770..4776)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4784..4800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4789..4811
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4808..4824
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4808..4824
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5167..5186
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    5185..5240
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5286..5305
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(5800..7299)
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="LLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPA
                     QTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKH
                     VPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVT
                     TTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKY
                     MFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHL
                     MGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRA
                     YRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKAR
                     DRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFAR
                     GPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     misc_feature    7311..7863
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7313..7886
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7473..7492)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     7702..7719
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(7912..8625,8626..8628,8629..8631))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7915..8625,8626..8628,8629..8631))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7957..7978)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8306..8325
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8565..8586
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8628..8637
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8672..8868)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8686..8821
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ccagaatacc tcagcctcca gcacctggca tgttgtagag caccaattca cagtcttcag
       61 gggtgtactg aaagctgggg actgtcagga gcctagaggt accgactcca taccctgagc
      121 agttcttagg cgaagccgct gagttgtgtg tggctcgcct ccactgttac accactgaac
      181 acactgacta gtgtggacca ggcacttccc aaggagagtc tcagaggtct gggaaggcct
      241 tgaaaaagtc ttctcaattt ttagaatgga aaagcagccc aaagaagtct aagtcatttc
      301 cagtgccaca gagttcacat ccgctgggag cctcaactcc cggcctcctc ttggctccac
      361 tcctgcccag gatctcaggg gtcaggggct gctcttgagg gttcttcatg gctctccatt
      421 cctactgatc cacttcccta aagtcccacc ggactctgag ctctacatct ccctgtccac
      481 tcactcagtt cctcaggagg tcctggagac cccagtagtg gggagagagg tccttggtgc
      541 ctcttagctg tccgctagct cagagctgta gggaggctgt tgcggtgcac agcactgatc
      601 ctaaccaagt ttgccagccc agcttttctc atggctccca cacaatggtc tgctttgtgc
      661 ccacctgccg ataatgctta attaggcgag gagttgatta atttgaggta attgacagct
      721 aattagaagc ctacaggctc attaacttga catcttgtct ttgtgaattg gctgggtacg
      781 gtcaggtgat ggtaaccgtt gctcccctcc ccagctcttc caaccccaaa gtctgaggca
      841 tcacctaggg gagggctggg cagatccggg gttcaggtag ggcagtctca agatcaagag
      901 tagagggaac tggtactgaa gagtaaggag tcccgaatgg agacgtctgc tctggctcta
      961 gggaggagag agaacaagaa tgaagggcaa atggacccga gagagaactg gaccctagca
     1021 ggagaatggg gaagcccgaa gctcacatag tgagaagggg ctggggagca ggtgagcctg
     1081 ttggtactag gggtaggctt tgaagagagg ccgacttgtg gttaaatccc agctctgcct
     1141 agagctagga gactccaact gacctaaatg ctctctaagc ccggggctcg tttgtaaaac
     1201 aggataacaa tgtctacctt acctcatttg gttactgtga acagttcatg ggacacagag
     1261 gcaaggccta ggaagcaccc agttaaatgt cactgactgg gctggcagga tgggtcagtg
     1321 ggtgcaaact tgatgaccca agcttgatcc ctggaaccgg ggtggaagga gagaactgag
     1381 tctggaaggt ccctcccacc tgcacacttg cactgtggca aacatcttcc tgtgctcact
     1441 tacacatcac acacactcac tctctctcac acacacacac acacaatgag taaaacctaa
     1501 agaatgttca ctatccttgg cagtgggaag acgtgactgg gagatagtct aagagggcag
     1561 gttgtcagag gcaatgggaa gcccctccct tggagaacta gtgtcactgt ggacatttgc
     1621 attcacagca caagagtgtt tgatggtgtg gggatcaggt cttcttgtcg caacctcttt
     1681 agggggcaat ttagtgatct atattacaat gtatatgtag cttttgaccc aagagttccc
     1741 tgctattaac gcactctgag gatttacatg tatgcgcaag gatttcatta cagcgcagcc
     1801 tgcagaacct tcctgcgtat cccagttgga aagactggaa atcacccaaa agtccatcag
     1861 cgactaagtt atggtacctc cccgtagtac agtatattac tattgaatat atatgagcag
     1921 gatccaaaat gcattaaata gggggaaaag ccacgtatat aacagtgtat agttcccaca
     1981 agtagctcag tggatggagg tacaggaaat gggaggcgta tttttggctt gtacttatca
     2041 ccttttcaaa aatcagttta caattaaccc ccccaccccc cataagccct aacaatactg
     2101 ctataatggc agagtagcgc catgaacaag ggcgggaaca cttggtttct tcttctacac
     2161 agacaaacat ggtggccatt ggccacgtgt ggccattgaa cccttgaaat gtggtctttt
     2221 tttttttttt ttttttttaa gaggatggag aggtggttca gtggttagaa gtacgtactg
     2281 ctcttgtgaa tttggttctt ttttttattt atttatttag tcgacccagg tggcactttt
     2341 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2401 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2461 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2521 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2581 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2641 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2701 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2761 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2821 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2881 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2941 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3001 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3061 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3121 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3181 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3241 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3301 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3361 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3421 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3481 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3541 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3601 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3661 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3721 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3781 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3841 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3901 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3961 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4021 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4081 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4141 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4201 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4261 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcggatcc
     4321 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     4381 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4441 taaacaagtt agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     4501 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     4561 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     4621 cgggactatg gttgctgact aattgagatg cggatccgct ggcacgacag gtttcccgac
     4681 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     4741 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     4801 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgggctgca ggtcgaggga
     4861 tctccataag agaagaggga cagctatgac tgggagtagt caggagagga ggaaaaatct
     4921 ggctagtaaa acatgtaagg aaaattttag ggatgttaaa gaaaaaaata acacaaaaca
     4981 aaatataaaa aaaatctaac ctcaagtcaa ggcttttcta tggaataagg aatggacagc
     5041 agggggctgt ttcatatact gatgacctct ttatagccaa cctttgttca tggcagccag
     5101 catatgggca tatgttgcca aactctaaac caaatactca ttctgatgtt ttaaatgatt
     5161 tgccctccca tatgtccttc cgagtgagag acacaaaaaa ttccaacaca ctattgcaat
     5221 gaaaataaat ttcctttatt agccagaagt cagatgctca aggggcttca tgatgtcccc
     5281 ataatttttg gcagagggaa aaagatctca gtggtatttg tgagccaggg cattggccac
     5341 accagccacc accttctgat aggcagcctg cacctgagga gtgcggccgg ccgcgatatc
     5401 ctcgacccca ctgggctgta ggtggtggct ggatggcctg agccccctcc tttcctgggg
     5461 tgcaggcagc cacagggcac aacctgcagt ccctggggtt ggggtaggga gtccgagagc
     5521 agccaagggg caggtttggt gtcatgggct cagtccccag agcgcacggg gaggggaagg
     5581 ttccatggca ggacctctgt atagggactc cggggatgac gactcgggct ggaggccagg
     5641 caggacgaca ggaggtgggg agcaggagaa ctccgggatg gggaagcagg agccccaggg
     5701 acgggacact cagggagcag tggccgtctc cagcagcagc agggtcccgg ccagcagagg
     5761 aagcaacgcg gggaccacgg accgccccgg gtgcgcggcg tcggtggtgc cggcgggggg
     5821 cgccaggtcg caggcggtgt agggctccag gcaggcggcg aaggccatga cgtgcgctat
     5881 gaaggtctgc tcctgcacgc cgtgaaccag gtgcgcctgc gggccgcgcg cgaacaccgc
     5941 cacgtcctcg cctgcgtggg tctcttcgtc caggggcact gctgactgct gccgatactc
     6001 ggggctcccg ctctcgctct cggtaacatc cggccgggcg ccgtccttga gcacatagcc
     6061 tggaccgttt ccgtatagga ggaccgtgta ggccttcctg tcccgggcct tgccaggggc
     6121 cagcccgaag atggagctcc ctcgcagggg gtagcctccg aaggagaaga cgtgggagtg
     6181 gtcggcagtg acgaggctca gcgtgtcctc ctcgctggtg agctggcccg ccctctcaat
     6241 ggcgtcgtcg aacatgatcg tctcagtcag tgcccggtaa gccctgcttt catgatgacc
     6301 atggtcgatg cgaccaccct ccacgaagag gaagaagccg cgggggttcc tgctcagcag
     6361 gcgcagggca gcctctgtca tctccatcag ggaggggtcc agtgtggagt ctcggtggat
     6421 ctcgtatttc atgtctccag gctcaaagag acccatgaga tgggtcacag acgggtccag
     6481 ggaagcctgc atgagctcag tgcggttcca cacataccgg gcaccctggc gcttcgccag
     6541 ccattcctgc accagattct tcccgtccag cctggtccca ccttggctgt agtcatctgg
     6601 gtactcaggg tctggggttc ccatgcgaaa catgtacttt cggcctccac ctaggatcac
     6661 gtcaatgtcc atgttggaga tgagctgcgt agcgatgtcc tggcacccct cctggcgggc
     6721 cgaggcaggc acgtcggcgt ccgagtacca gttgcggttc accgtgtggg cgtaggtgcc
     6781 ggctggcgag gcgtgctgca ctcgtgtggt ggttaccact cccactgact tccctgcttt
     6841 cttggcccga ttcatcacgg agatgacctc gttgccgcgt gtcgtgttgc actggttaaa
     6901 gcgggcggct gcactcaagc caatggtctg gaagttgccc ttgaccccgc acaggtaggc
     6961 cgtggctgtg gctccactgt ctggcacatg tttgtctaca ttgtatgtct tggacagagc
     7021 cacatatggg aagcggtcca tggccagggg tatctcaggc cccagtttgt ccttcttctg
     7081 cccttttagg atcctggcag ctgtcaccgt agacaccccc atcccatcgc ccaggaagat
     7141 gatgaggttc ttggcggctg tctgtgcagg ctgcagcttc ttggcggcac ccagggcctc
     7201 ggctgcctcg cggttccaga agtccgggtt ctcctcctca actgggatga tgcccaggga
     7261 gagctgtagc ctcaggccca gcagcagcag cagcagcagc ctcgacccca tattatcatc
     7321 gtgtttttca aaggaaaacc acgtccccgt ggttcggggg gcctagacgt ttttttaacc
     7381 tcgactaaac acatgtaaag catgtgcacc gaggccccag atcagatccc atacaatggg
     7441 gtaccttctg ggcatccttc agccccttgt tgaatacgct tgaggagagc catttgactc
     7501 tttccacaac tatccaactc acaacgtggc actggggttg tgccgccttt gcaggtgtat
     7561 cttatacacg tggcttttgg ccgcagaggc acctgtcgcc aggtgggggg ttccgctgcc
     7621 tgcaaagggt cgctacagac gttgtttgtc ttcaagaagc ttccagagga actgcttcct
     7681 tcacgacatt caacagacct tgcattcctt tggcgagagg ggaaagaccc ctaggaatgc
     7741 tcgtcaagaa gacagggcca ggtttccggg ccctcacatt gccaaaagac ggcaatatgg
     7801 tggaaaataa catatagaca aacgcacacc ggccttattc caagcggctt cggccagtaa
     7861 cgttaggggg gggggaggga gagggggggg gggggggggg aattggccgc tttacttgta
     7921 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
     7981 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
     8041 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     8101 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
     8161 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
     8221 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
     8281 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     8341 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     8401 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
     8461 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     8521 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     8581 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcgac
     8641 cggtggatcc cgggcccgcg gtaccaagct ttttgcaaaa gcctaggcct ccaaaaaagc
     8701 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     8761 aaaaaattag tcagcgatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     8821 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcaga tcgcagatct
     8881 cgagctggca tgcatgtata tgtgtactgg catgcgtgta tatatgtact ggcatgtgtg
     8941 taaaccttgc gcttaggagg ctgaacagga ggactgtgag ttcaaggaca gcctagacta
     9001 tacactgagt ctctggctaa acaaatactg taaacacagc atctatcttg ataaacaacc
     9061 cattaaacat acctatggac acaaataata tattcaccaa ttttttttat atgctattcc
     9121 tgccagcaca cagtaggcac ttcctaaggg cctactgtcc ctttcatctc cagggcacaa
     9181 cattaacaat tctgtttgcc cagccacctc cctttccact gtgacccttg atcagcaagc
     9241 acaataaatg tttgctaggt gactgattta taccagggat gagggaacgt ctccaagctt
     9301 ccacagaagt gaaagacaag tggcctccat cgccccacaa gggaagatga tgtcaggtgc
     9361 cagggtggag atggtgaaca tactcggtgt gctggagtcc cagcagccaa gctcaccctg
     9421 tcccagggtc aaaagtattc agctgccagg accatatcag aaatgtaatc tcagtaccca
     9481 aggtctgtcc taaggcctgg ctccttaggc cttgcacctc ccacctaagt tggacagggc
     9541 cctttcttcc acttctttag ttggagatgg gtgtttaact catatgatga gccatgtatt
     9601 gtatcagggc tctttgtgag tggagaggga cacctgagcc ctgggtaccc aaaggtgtag
     9661 agagatgggg acacagatgt ag
//
