LOCUS       sequence-446955-        8595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8595)
  TITLE     Tet-On Advanced vectors
REFERENCE   2  (bases 1 to 8595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMVtight eGFP Puro (w771-1)
FEATURES             Location/Qualifiers
     source          1..8595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    290..415
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             462..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(643..869)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(897..917)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(897..915)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(936..952)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(936..952)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(949..971)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    960..976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(984..990,991..1008,1009..1014))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1029..1050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1167..1184)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1338..1926)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1418..1437)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2097..2888,2889..2957))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2720..2739
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2958..3062)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3088..3543)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3225..3246)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3437..3456
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3670..3692
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3684..3701
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3685..3701
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3711..3730
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3711..3729
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3750..3885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3925..4046
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3936..3955)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3990..4009
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4118..4351
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4489..5088)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4572..4592)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5069..5088
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5109..5608)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5157..5176)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5550..5573
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    5622..6210
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5728..5739
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6137..6157
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    6241..6265
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(6290..7003,7004..7006,7007..7009))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6293..7003,7004..7006,7007..7009))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6335..6356)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6684..6703
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6943..6964
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      7006..7015
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7027..7079
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(7108..7132)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(7210..7524)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(7213..7237)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7284..7302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7320..7338
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7355..7373
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7391..7409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7427..7445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7462..7480
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7498..7516
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    7546..7663
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7930..7974)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8159..8392
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta
       61 ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc
      121 ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt
      181 ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc
      241 ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc
      301 acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc
      361 gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct
      421 ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac
      481 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
      541 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
      601 agagagctcc caggctcaga tctggtctaa ccagagagac ccgtttatgt atcgagctag
      661 gcacttaaat acaatatctc tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag
      721 acccgtctgt tgccttccta ataaggcacg atcgtaccac cttacttcca ccaatcggca
      781 tgcacggtgc tttttctctc cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca
      841 agactacaag agtattgcat aagactacat taagcttgca gctccagctt ttgttccctt
      901 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      961 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1021 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1081 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1141 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1201 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1261 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1321 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1381 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1441 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1501 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1561 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1621 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1681 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1741 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1801 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1861 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1921 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1981 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2041 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2101 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2161 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2221 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2281 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2341 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2401 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2461 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2521 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2581 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2641 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2701 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2761 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2821 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2881 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2941 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3001 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3061 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     3121 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3181 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3241 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3301 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3361 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3421 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3481 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     3541 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     3601 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     3661 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     3721 cactataggg cgaattgggt acgtccctag gcctccaaaa aagcctcctc actacttctg
     3781 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     3841 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     3901 cgggatagct agagccagac atgataagat acattgatga gtttggacaa accacaacta
     3961 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     4021 ccattataag ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt
     4081 tataaatact gaataataag atgacatgaa ctactactgc tagagatttt ccacactgac
     4141 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     4201 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     4261 agctcccagg ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct
     4321 tgtcttcgtt gggagtgagc tagcccttcc agtcccccct tttcttttaa aaagtggcta
     4381 agatctacag ctgccttgta agtcattggt cttaaaggta ccgggccctc gagatgcatg
     4441 gggtcgtgcg ctcctttcgg tcgggcgctg cgggtcgtgg ggcgggcgtc aggcaccggg
     4501 cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt
     4561 gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg
     4621 caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt
     4681 gccctggtgg tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg
     4741 gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc
     4801 ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt
     4861 ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac
     4921 gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac
     4981 ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg
     5041 gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg taagcttggg
     5101 ctgcaggtcg aaaggcccgg agatgaggaa gaggagaaca gcgcggcaga cgtgcgcttt
     5161 tgaagcgtgc agaatgccgg gcctccggag gaccttcggg cgcccgcccc gcccctgagc
     5221 ccgcccctga gcccgccccc ggacccaccc cttcccagcc tctgagccca gaaagcgaag
     5281 gagcaaagct gctattggcc gctgccccaa aggcctaccc gcttccattg ctcagcggtg
     5341 ctgtccatct gcacgagact agtgagacgt gctacttcca tttgtcacgt cctgcacgac
     5401 gcgagctgcg gggcgggggg gaacttcctg actaggggag gagtagaagg tggcgcgaag
     5461 gggccaccaa agaacggagc cggttggcgc ctaccggtgg atgtggaatg tgtgcgaggc
     5521 cagaggccac ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca tgctccagac
     5581 tgccttggga aaagcgcctc ccctacccgg tagaattcca ggcggggagg cggcccaaag
     5641 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
     5701 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
     5761 cccgcgcaga atccaggtgg caacacaggc gagcagccat ggaaaggacg tcagcttccc
     5821 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
     5881 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
     5941 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
     6001 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
     6061 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
     6121 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
     6181 ttcacaaatt ttgtaatcca gaggttgatt gtcgagcggc cgccactgtg ctggatatca
     6241 accactttgt acaagaaagc tgggtctaga tatctcgagt gcggccgctt tacttgtaca
     6301 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
     6361 cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg
     6421 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
     6481 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct
     6541 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
     6601 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
     6661 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
     6721 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
     6781 cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg
     6841 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
     6901 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
     6961 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg
     7021 gccggtggat cccgggcccg cggtaccgtc gactgcagaa ttcgaagctt gagctcgaga
     7081 tccagtcgac tgaattggtt cctttaaagc ctgctttttt gtacaaactt gttgatatct
     7141 gcagaattcc accacactgg actagtcgac gaaccggtgg atccccgggt accgagctcg
     7201 aattctccag gcgatctgac ggttcactaa acgagctctg cttatatagg cctcccaccg
     7261 tacacgccta cctcgacata cgttctctat cactgatagg gagtaaactc gacatacgtt
     7321 ctctatcact gatagggata aactcgacat acgttctcta tcactgatag ggagtaaact
     7381 cgacatacgt tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat
     7441 agggagtaaa ctcgacatcg ttctctatca ctgataggga gtaaactcga catacgttct
     7501 ctatcactga tagggagtaa actcgaggct agtctcgtga tcgataaaat tttgaatttt
     7561 tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct
     7621 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaagttaacc gataccgtcg
     7681 agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat
     7741 tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga
     7801 atatccctgc ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac
     7861 caagcctcct actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa
     7921 ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg
     7981 ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct
     8041 gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact
     8101 agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc aaatccccag
     8161 gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga gctgcttgat
     8221 gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca gcaaattgtt
     8281 ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg tcattgacgc
     8341 tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc ctattcccac
     8401 tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta ctcctaatgg
     8461 ttcaattttt actactttat atttatataa ttcacttctc caattgtccc tcatatctcc
     8521 tcctccaggt ctgaagatca gcggccgctt gctgtgcggt ggtcttactt ttgttttgct
     8581 cttcctctat cttgt
//
