LOCUS       sequence-446972-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This is the native promoter from the ermC erythromycin resistance 
            cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  TITLE     Cultivarium plasmids
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGLU_64
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(644..660)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(644..660)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(657..679)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(832..1420)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(912..931)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1553..1647)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1668..2327)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2242..2261
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2328..2430)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2517..2539
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2531..2548
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2532..2548
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtgactcagg tctcacgtaa tcgacaaagg gaagcatgcg ttccgcagcc acacgccgtg
       61 tgaagctggg atcctttaac tctggcaacc ctcaaaattg aatgagacat gctacacctc
      121 cggataataa atatatataa acgtatatag atttcataaa gtctaacaca ctagacttat
      181 ttacttcgta attaagtcgt taaaccgtgt gctctacgac caaaactata aaacctttaa
      241 gaactttctt tttttacaag aaaaaagaaa ttagataaat ctctcatatc ttttattcaa
      301 taatcgcatc cgattgcagt ataaatttaa cgatcactca tcatgttcat atttatcaga
      361 ggtcgtgcta taattatact aattttataa ggaggaaaaa atatgggcat ttttagtatt
      421 tttgtaatca gcacagttca ttatcaacca aacaaaaaat aagtggttat aatgaatcgt
      481 taataagcaa aattcatata accaaattaa agagggttat aaatgtgaga ccgggattca
      541 cattggatac gagatccgta gattgataag aggacacgga atatcccagg ctaggtggag
      601 gctcagtgat gataagtctg cgatggtgga tgcatgtgtc atggtcatag ctgtttcctg
      661 tgtgaaattg ttatccgctc agagggcaca atcctattcc gcgctatccg acaatctcca
      721 agacattagg tggagttcag ttcggcgtat ggcatatgtc gctggaaaga acatgtgagc
      781 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      841 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      901 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      961 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1021 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1081 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1141 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1201 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1261 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1321 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1381 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1441 tacggggtct gacgctctat tcaacaaagc cgccgtcccg tcaagtcagc gtaaatgggt
     1501 agggggcttc aaatcgtccc cccatacgat ataagttgtt actagtgctt ggattctcac
     1561 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     1621 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     1681 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     1741 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     1801 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     1861 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     1921 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     1981 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     2041 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     2101 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     2161 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     2221 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     2281 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     2341 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     2401 tgaaagttgg aacctcttac gtgccgatca acgtctcatt gatacctgaa acaaaaccca
     2461 tcgtacggcc aaggaagtct ccaataactg tgatccacca caagcgccag ggttttccca
     2521 gtcacgacgt tgtaaaacga cggccagtca tgcataatcc gcacgcatct ggaataagga
     2581 agtgccattc cgcctgacct
//
