LOCUS       sequence-44698-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Subach OM, Entenberg D, Condeelis JS, Verkhusha VV
  TITLE     A FRET-facilitated photoswitching using an orange fluorescent 
            protein with the fast photoconversion kinetics.
  JOURNAL   J Am Chem Soc. 2012 Aug 17.
  PUBMED    22900938
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2012 Aug 17."
COMMENT     Sequence Label: pPSmOrange2-N1
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(129..836)
                     /codon_start=1
                     /product="enhanced orange to far-red photoswitchable 
                     monomeric fluorescent protein (Subach et al., 2012)"
                     /label=PSmOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGTVNGHEFEIEGEGEGHPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLITYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVLLPGAYIVGIKLDITSHNEDYTIVEQYERS
                     EARHSTGGMDELYK"
     primer_bind     complement(206..229)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     regulatory      833..842
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    844..924
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgcttact tgtacagctc gtccatgccg ccggtggagt ggcgggcctc ggagcgttcg
      181 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt gatgccgacg
      241 atgtaggcgc cgggcagcag cacggacttc ttggccttgt aggtagtctt gacctcggag
      301 gtgtagtggc cgccatcctt cagcttcagc ctcatcctga tctcgccctt cagggcgccg
      361 tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt ctgcatcacg
      421 gggccgtcgg aggggaagtt ggtgccgcgc atcttcacct tgtagatgaa ctcgccgtcc
      481 tgcagagagg agtcctgggt cacggtcacc acgccgccgt cctcgtagtt catcacgcgc
      541 tcccacttga agccctcggg gaaggacagc ttgaagtagt cggggatgtc ggcggggtgc
      601 ttcacgtagg ccttggagcc gtaggtgatg agaggggaca ggatgtccca ggcgaagggc
      661 agggggccgc ccttggtcac cttcagctta gcggtctgaa agccctcgta ggggtggccc
      721 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacag tgccctccat gtgcaccttg
      781 aagcgcatga actccttaat gatggccata ttattctcct cgcccttgct caccatggtg
      841 gcgaccggtg gatcccgggc ccgcggtacc gtcgactgca gaattcgaag cttgagctcg
      901 agatctgagt ccggtagcgc tagcggatct gacggttcac taaaccagct c
//
