LOCUS       sequence-446985-        9916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Honnell V, Sweeney S, Norrie J, Parks M, Ramirez C, Jannu AJ, Xu B, 
            Teubner B, Lee AY, Bell C, Dyer MA
  TITLE     Evolutionary conservation of VSX2 super-enhancer modules in retinal 
            development.
  JOURNAL   Development. 2024 Jul 1;151(13):dev202435. doi: 10.1242/dev.202435. 
            Epub 2024 Jul 12.
  PUBMED    38994775
REFERENCE   2  (bases 1 to 9916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2024-07-1"; volume: "151(13):dev202435. doi";
            pages: " 10.1242/dev.202435. Epub 2024 Jul 12"
COMMENT     Sequence Label: Vsx2 mR2-22 +SV40 base prom-GFP-IRES-AP
FEATURES             Location/Qualifiers
     source          1..9916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        747..943
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      794..929
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      978..987
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(984..986,987..989,990..1703)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(984..986,987..989,990..1700)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1029..1050)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1290..1309)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1637..1658
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1730..2303
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1753..2305
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1897..1914)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2124..2143
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2317..3816
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="LLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPA
                     QTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKH
                     VPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVT
                     TTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKY
                     MFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHL
                     MGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRA
                     YRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKAR
                     DRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFAR
                     GPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     primer_bind     complement(4311..4330)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4376..4431
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4430..4449)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4792..4808)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4792..4808)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4805..4827)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4816..4832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4840..4846,4847..4864,4865..4870))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4885..4906
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4965..5160
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5011..5146
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5166..5300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5203..5222)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5257..5276
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5368..5385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5539..6127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5619..5638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6298..7089,7090..7158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6921..6940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7159..7263)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgagagctgc ttgaggggag gggccccggc tttttccccc ctgagaacca gcagctcaat
       61 aaataaagat atcaaccccc tcctgtgacc gccttctgca ggttataaat cctcctgctg
      121 ctctcccaaa acaacaccct caaaaacatg gacgactggc tggcaaggtg gctcagcagg
      181 taaaggcact cacttgccac caatcctgat cacctgagtt ccatcccaag gaccccactg
      241 ggtgttcctc acacatgata aataaaagat ttttaaattt tatttattta tttatttatt
      301 tttaaataca agtcagagtt gttcaggacg tctgcccatc actctgtcca tcggtctgac
      361 taaacagttt tgaataaaga tgggtgcatg gtgcggtgga aagaagctaa gaggacaggt
      421 tgaagtctga ctagagggga taagcctcag cttcctgtat gacccgggca tgttactaaa
      481 tctctgtgtg cctcagtttg ctcttctgta aagtgggtgt tcgtcatagc agtgcccacc
      541 tccaagagtt ctgtgaggat gaaagaatgg aggaaaagca tttagtacta tagagtggcc
      601 actaagccag cacccagaac ttgtcctact tcctgcttag tctctgtttg cctgtctgct
      661 tccaaagatg agttactaga attcaaggaa tggctttttt ttaaagattt atttatttat
      721 tatatgtaag ctcgagatct gcgatctgca tctcaattag tcagcaacca tagtcccgcc
      781 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatcg
      841 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
      901 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gtaccgcggg
      961 cccgggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     1021 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1081 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1141 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     1201 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1261 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1321 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1381 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     1441 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     1501 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1561 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     1621 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     1681 gcatggacga gctgtacaag taaagcggcc aattcccccc cccccccccc ccctctccct
     1741 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     1801 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     1861 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     1921 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     1981 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     2041 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     2101 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     2161 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     2221 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     2281 tcctttgaaa aacacgatga taatatgggg tcgaggctgc tgctgctgct gctgctgggc
     2341 ctgaggctac agctctccct gggcatcatc ccagttgagg aggagaaccc ggacttctgg
     2401 aaccgcgagg cagccgaggc cctgggtgcc gccaagaagc tgcagcctgc acagacagcc
     2461 gccaagaacc tcatcatctt cctgggcgat gggatggggg tgtctacggt gacagctgcc
     2521 aggatcctaa aagggcagaa gaaggacaaa ctggggcctg agatacccct ggccatggac
     2581 cgcttcccat atgtggctct gtccaagaca tacaatgtag acaaacatgt gccagacagt
     2641 ggagccacag ccacggccta cctgtgcggg gtcaagggca acttccagac cattggcttg
     2701 agtgcagccg cccgctttaa ccagtgcaac acgacacgcg gcaacgaggt catctccgtg
     2761 atgaatcggg ccaagaaagc agggaagtca gtgggagtgg taaccaccac acgagtgcag
     2821 cacgcctcgc cagccggcac ctacgcccac acggtgaacc gcaactggta ctcggacgcc
     2881 gacgtgcctg cctcggcccg ccaggagggg tgccaggaca tcgctacgca gctcatctcc
     2941 aacatggaca ttgacgtgat cctaggtgga ggccgaaagt acatgtttcg catgggaacc
     3001 ccagaccctg agtacccaga tgactacagc caaggtggga ccaggctgga cgggaagaat
     3061 ctggtgcagg aatggctggc gaagcgccag ggtgcccggt atgtgtggaa ccgcactgag
     3121 ctcatgcagg cttccctgga cccgtctgtg acccatctca tgggtctctt tgagcctgga
     3181 gacatgaaat acgagatcca ccgagactcc acactggacc cctccctgat ggagatgaca
     3241 gaggctgccc tgcgcctgct gagcaggaac ccccgcggct tcttcctctt cgtggagggt
     3301 ggtcgcatcg accatggtca tcatgaaagc agggcttacc gggcactgac tgagacgatc
     3361 atgttcgacg acgccattga gagggcgggc cagctcacca gcgaggagga cacgctgagc
     3421 ctcgtcactg ccgaccactc ccacgtcttc tccttcggag gctaccccct gcgagggagc
     3481 tccatcttcg ggctggcccc tggcaaggcc cgggacagga aggcctacac ggtcctccta
     3541 tacggaaacg gtccaggcta tgtgctcaag gacggcgccc ggccggatgt taccgagagc
     3601 gagagcggga gccccgagta tcggcagcag tcagcagtgc ccctggacga agagacccac
     3661 gcaggcgagg acgtggcggt gttcgcgcgc ggcccgcagg cgcacctggt tcacggcgtg
     3721 caggagcaga ccttcatagc gcacgtcatg gccttcgccg cctgcctgga gccctacacc
     3781 gcctgcgacc tggcgccccc cgccggcacc accgacgccg cgcacccggg gcggtccgtg
     3841 gtccccgcgt tgcttcctct gctggccggg accctgctgc tgctggagac ggccactgct
     3901 ccctgagtgt cccgtccctg gggctcctgc ttccccatcc cggagttctc ctgctcccca
     3961 cctcctgtcg tcctgcctgg cctccagccc gagtcgtcat ccccggagtc cctatacaga
     4021 ggtcctgcca tggaaccttc ccctccccgt gcgctctggg gactgagccc atgacaccaa
     4081 acctgcccct tggctgctct cggactccct accccaaccc cagggactgc aggttgtgcc
     4141 ctgtggctgc ctgcacccca ggaaaggagg gggctcaggc catccagcca ccacctacag
     4201 cccagtgggg tcgaggatat cgcggccggc cgcactcctc aggtgcaggc tgcctatcag
     4261 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     4321 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     4381 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     4441 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     4501 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     4561 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     4621 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     4681 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     4741 cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct
     4801 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     4861 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     4921 actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca
     4981 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5041 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5101 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5161 aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5221 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5281 tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5341 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5401 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5461 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5521 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5581 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5641 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5701 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5761 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5821 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5881 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5941 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6001 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6061 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6121 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6181 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6241 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6301 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6361 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     6421 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     6481 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     6541 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     6601 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     6661 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     6721 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6781 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6841 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6901 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6961 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7021 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7081 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7141 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     7201 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     7261 gcgcacattt ccccgaaaag tgccacctgg gtcgacgaat cttataataa aagtcattat
     7321 tttgtataat taatatatac taataaagga cattttaaaa ataaagaatt gaggcccagg
     7381 tctgtgacct caactttgga gggcagtgtg gagcctggca ctaaaggccc cacactgccc
     7441 tctcttgtga aaatcatttt gtcagttgga accaaggggc taacaaacct atccagggtc
     7501 acacagctgt caaacccagg gacctgcctc cagacctccc tgcctccaaa attctccctc
     7561 agagggtctg tagtatcatc accgtagcct tctatcctgg gaatccaagg cagagggcaa
     7621 tgacaaagca tagtgtgtgg gtggccccat ctgtcagggg tgggcagagg gctaagcttc
     7681 ggaggccaga cccttggcag atgaccctag caggaatgcc aagggctcta gggaggcaga
     7741 gggtgccagc ctgcccccat gagagaaggg gactgctggt aactctctcc caggcatggt
     7801 gacagctgca gtgtagggca ggacagaggc cccacatggg aagaactttc actcactctg
     7861 cctggggcct aatctcatta ctctgcatgg accctctagg accaccctct ctagtctctt
     7921 ttaatcccct ccctccctct ggggatactc caaggtctgc cccttgccag gtcaggccag
     7981 cagaggcaca tgcaggcccc acccttcagt tttggcagac tacctgtgcc ccacttctac
     8041 agtcctcaaa gtagggccag gaagggctgg agagatggct cagcagttaa aagcacagac
     8101 tgctctttca aaggtcctga gttcaaatcc cagcaaccac atagtggctc acaaccaccc
     8161 gtaatgagat ctgacactct cttctggtat gtctgaagtc agctacagtg aacttatata
     8221 taacaataaa taaattgaag aagaagaaga aaaagaagaa aagggggagc caggaaggct
     8281 gaaggtccag tttctctcac ctgcctgtag cagtctgaca tgccttcctg aaatggcctt
     8341 cttctggact ccctgtttct cttgtcctcc accctaccct gcacccctgg ctggaaatac
     8401 ccagagctac cctgtgctag acaagtgttc tacctccctc tcctttctaa gcagctccca
     8461 ggacttttgt ttgggttccc atttcaggac tgactgatgg aaagagaccc catgtccaca
     8521 gtaaaactga gacagcaact cccccttcga atagctgttc tgaagccaga agacactgca
     8581 tattgggtgc tcagccagcc cctggcagag ggatcctcaa cgtggctttt atcagggata
     8641 caggttgtca acatcacatg tgccaaagat gtctcctggg ctgaggtcaa gtgtgggagg
     8701 aaggttggga ttcagtgtgt ataatcctcc taagagtgct gggtgcccct ggctttgaac
     8761 tgagttcctt actaccagct ctcttacagg acaggtcctg tattgctccc attttgcaga
     8821 tgtgagaact gaggttaacg gacagcttgt ggggagaagg agctgcaaca gaattctcca
     8881 aagcaatcta ttgcctctca ggacacacta aaaaacagga cagatgccca aaacaactac
     8941 ccccccccat agtaatatgc tccctgttct ctagaaggat tcagtgtaat gccccccccc
     9001 cgcaaggact ggcccgaaac cccatcacag ccatccaggg tccagcgctg tccctcctca
     9061 ccctgagcat ccaaggggtc cttttggctc tgggtctccc tctctcccac cttctgctgc
     9121 tgttcctcat ttacataaat tatctcttcc ccaattatct atccagctgt cccaacggtg
     9181 atctgtgaat gaattaaaaa cggaatataa tttaataatg gctgattaga agaagattag
     9241 ttgttatata aaaaggaaaa gccaagagcc agttagttgc ccttaatctg agccaatacc
     9301 atcgttattc aggaaagctg ccctggccac caccgggcaa ggcagacatg ggggccaggg
     9361 taggaagagc tcaggggtgg gtggcagacc accccagaga caagagccta aggttgattc
     9421 ccacgacagc acacagaccg ggtgggtggc tgctatgact cccttctaag cggtttgagc
     9481 cgtgtctgga ggtctattca tgccctcaat tctaggcagc caggacgaaa accaccagat
     9541 tctcccgagg catccacggt gctcatatgg gccccgaacc acccctaagc aatttctgct
     9601 cagtctagga tacaggattc ccagagcaca gggcagttgg gtacacagat ctccctacca
     9661 aggaatctgc ttctctggag taggtggtga gcgaaagaat tgcctggact agcctggact
     9721 agcctgggag ggactggccc agacagggga cctggcccag ggaagggaat ctggggtttc
     9781 cttttagcat tgcaccctca cttcaagtga accttctcca ggaagcctgg gctgacgccc
     9841 ccaatctccc atgctatgac ttcctccatc aagaactgtg gccccctctg tgtgagcggc
     9901 tctcagcccc ctactg
//
