LOCUS       sequence-446995-       14704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14704)
  AUTHORS   Honnell V, Sweeney S, Norrie J, Parks M, Ramirez C, Jannu AJ, Xu B, 
            Teubner B, Lee AY, Bell C, Dyer MA
  TITLE     Evolutionary conservation of VSX2 super-enhancer modules in retinal 
            development.
  JOURNAL   Development. 2024 Jul 1;151(13):dev202435. doi: 10.1242/dev.202435. 
            Epub 2024 Jul 12.
  PUBMED    38994775
REFERENCE   2  (bases 1 to 14704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2024-07-1"; volume: "151(13):dev202435. doi";
            pages: " 10.1242/dev.202435. Epub 2024 Jul 12"
COMMENT     Sequence Label: Vsx2 R0+R1+R3 +SV40 base prom-GFP-IRES-AP
FEATURES             Location/Qualifiers
     source          1..14704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    828..962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(852..871)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     906..925
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(968..1163)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      982..1117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    1222..1243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1258..1263,1264..1281,1282..1288)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1296..1312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1301..1323
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1320..1336
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1320..1336
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1679..1698
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1697..1752
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1798..1817
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(2312..3811)
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="LLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPA
                     QTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKH
                     VPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVT
                     TTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKY
                     MFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHL
                     MGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRA
                     YRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKAR
                     DRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFAR
                     GPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     misc_feature    3823..4375
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3825..4398
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3985..4004)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4214..4231
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(4425..5138,5139..5141,5142..5144))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4428..5138,5139..5141,5142..5144))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4470..4491)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4819..4838
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5078..5099
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5141..5150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5185..5381)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5199..5334
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        13569..13673
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13674..13742,13743..14534)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13892..13911)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga
      841 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      901 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      961 ttagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     1021 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     1081 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     1141 tggttgctga ctaattgaga tgcggatccg ctggcacgac aggtttcccg actggaaagc
     1201 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     1261 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     1321 aggaaacagc tatgaccatg attacgccaa gcttgggctg caggtcgagg gatctccata
     1381 agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat ctggctagta
     1441 aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa caaaatataa
     1501 aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca gcagggggct
     1561 gtttcatata ctgatgacct ctttatagcc aacctttgtt catggcagcc agcatatggg
     1621 catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga tttgccctcc
     1681 catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca atgaaaataa
     1741 atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc ccataatttt
     1801 tggcagaggg aaaaagatct cagtggtatt tgtgagccag ggcattggcc acaccagcca
     1861 ccaccttctg ataggcagcc tgcacctgag gagtgcggcc ggccgcgata tcctcgaccc
     1921 cactgggctg taggtggtgg ctggatggcc tgagccccct cctttcctgg ggtgcaggca
     1981 gccacagggc acaacctgca gtccctgggg ttggggtagg gagtccgaga gcagccaagg
     2041 ggcaggtttg gtgtcatggg ctcagtcccc agagcgcacg gggaggggaa ggttccatgg
     2101 caggacctct gtatagggac tccggggatg acgactcggg ctggaggcca ggcaggacga
     2161 caggaggtgg ggagcaggag aactccggga tggggaagca ggagccccag ggacgggaca
     2221 ctcagggagc agtggccgtc tccagcagca gcagggtccc ggccagcaga ggaagcaacg
     2281 cggggaccac ggaccgcccc gggtgcgcgg cgtcggtggt gccggcgggg ggcgccaggt
     2341 cgcaggcggt gtagggctcc aggcaggcgg cgaaggccat gacgtgcgct atgaaggtct
     2401 gctcctgcac gccgtgaacc aggtgcgcct gcgggccgcg cgcgaacacc gccacgtcct
     2461 cgcctgcgtg ggtctcttcg tccaggggca ctgctgactg ctgccgatac tcggggctcc
     2521 cgctctcgct ctcggtaaca tccggccggg cgccgtcctt gagcacatag cctggaccgt
     2581 ttccgtatag gaggaccgtg taggccttcc tgtcccgggc cttgccaggg gccagcccga
     2641 agatggagct ccctcgcagg gggtagcctc cgaaggagaa gacgtgggag tggtcggcag
     2701 tgacgaggct cagcgtgtcc tcctcgctgg tgagctggcc cgccctctca atggcgtcgt
     2761 cgaacatgat cgtctcagtc agtgcccggt aagccctgct ttcatgatga ccatggtcga
     2821 tgcgaccacc ctccacgaag aggaagaagc cgcgggggtt cctgctcagc aggcgcaggg
     2881 cagcctctgt catctccatc agggaggggt ccagtgtgga gtctcggtgg atctcgtatt
     2941 tcatgtctcc aggctcaaag agacccatga gatgggtcac agacgggtcc agggaagcct
     3001 gcatgagctc agtgcggttc cacacatacc gggcaccctg gcgcttcgcc agccattcct
     3061 gcaccagatt cttcccgtcc agcctggtcc caccttggct gtagtcatct gggtactcag
     3121 ggtctggggt tcccatgcga aacatgtact ttcggcctcc acctaggatc acgtcaatgt
     3181 ccatgttgga gatgagctgc gtagcgatgt cctggcaccc ctcctggcgg gccgaggcag
     3241 gcacgtcggc gtccgagtac cagttgcggt tcaccgtgtg ggcgtaggtg ccggctggcg
     3301 aggcgtgctg cactcgtgtg gtggttacca ctcccactga cttccctgct ttcttggccc
     3361 gattcatcac ggagatgacc tcgttgccgc gtgtcgtgtt gcactggtta aagcgggcgg
     3421 ctgcactcaa gccaatggtc tggaagttgc ccttgacccc gcacaggtag gccgtggctg
     3481 tggctccact gtctggcaca tgtttgtcta cattgtatgt cttggacaga gccacatatg
     3541 ggaagcggtc catggccagg ggtatctcag gccccagttt gtccttcttc tgccctttta
     3601 ggatcctggc agctgtcacc gtagacaccc ccatcccatc gcccaggaag atgatgaggt
     3661 tcttggcggc tgtctgtgca ggctgcagct tcttggcggc acccagggcc tcggctgcct
     3721 cgcggttcca gaagtccggg ttctcctcct caactgggat gatgcccagg gagagctgta
     3781 gcctcaggcc cagcagcagc agcagcagca gcctcgaccc catattatca tcgtgttttt
     3841 caaaggaaaa ccacgtcccc gtggttcggg gggcctagac gtttttttaa cctcgactaa
     3901 acacatgtaa agcatgtgca ccgaggcccc agatcagatc ccatacaatg gggtaccttc
     3961 tgggcatcct tcagcccctt gttgaatacg cttgaggaga gccatttgac tctttccaca
     4021 actatccaac tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt atcttataca
     4081 cgtggctttt ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg
     4141 gtcgctacag acgttgtttg tcttcaagaa gcttccagag gaactgcttc cttcacgaca
     4201 ttcaacagac cttgcattcc tttggcgaga ggggaaagac ccctaggaat gctcgtcaag
     4261 aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat ggtggaaaat
     4321 aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt aacgttaggg
     4381 gggggggagg gagagggggg gggggggggg cggaattggc cgctttactt gtacagctcg
     4441 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
     4501 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc
     4561 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
     4621 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
     4681 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
     4741 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
     4801 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
     4861 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
     4921 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
     4981 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
     5041 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
     5101 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc gaccggtgga
     5161 tcccgggccc gcggtaccaa gctttttgca aaagcctagg cctccaaaaa agcctcctca
     5221 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     5281 tagtcagcga tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     5341 agttaggggc gggactatgg ttgctgacta attgagatgc agatcgcaga tctcgagaga
     5401 catacgggga agccttcctg agtggggtag taacaggact ggactctgaa ggactggctt
     5461 acagggactt cctcatattt gacctgaagc aatgacacct tcaaccgctt taacctataa
     5521 ccacctcttc tcagtgcttt tcccctgaac aaaaggaaaa aaaaacatct tctactcagc
     5581 agacctcctc atggttcccc tcagccatcc tgaggtggct gctaggccct caagagccgg
     5641 gtatctccac cccatctcaa aattttctct tggcctgaaa tcaggggccg gagtaacttt
     5701 cctgcaatgc tgaagatgtt gggcagagag gaaattatgt ccatgtccct ggggccccac
     5761 cccacaatgc cccctgcagt gaggaacaac catgagggct gctcaagggg gaggtgaatg
     5821 aagaaacaca gctaggggag ggcttggtct cacaatccag ttccccttaa tatcccataa
     5881 taaggtgctg tcaacatgag tttcccgttc aagtagattt gctaagaagg ctgcgggagg
     5941 tcagaggtgc tttctggccc tcctggtatg gggcacaggg gcccttcctc accttttccc
     6001 cctctgcagt ctaaatagac tcatttgtca gcctgtgtgt acccgggttc catgaatccg
     6061 tgacggggtg ttagaggccc ttgagccctc tgtgtctcct ccgagataca aaaacaggat
     6121 tttgttttaa tgtgagggag ctcagagctt gaccaggaag gtaggcatgg aggtcccagg
     6181 gagacagggc tccacatgta gagggatcca gaatgcgttg gccgacctgg tgtttagatt
     6241 ggctgctggc tactcactct gctttcccat ggaatccctg ttaaacactc cctctttccc
     6301 cctggggtgg ggcagagacc tggcttctca ggtgtctctc tgtagaatgg tgtgagctct
     6361 tgaagagagt agtaaaggag acatggtcgc catgtaccag gccggctcta gagcgtccca
     6421 tatggaattg cttcctgcag ttcggtggag ctgggacagg cagaaacacc aaggggtcaa
     6481 ggctggggat ggaagctgtg taagaacagg ctgtggtacc tatttcccca agccagcagg
     6541 ggctgttgga atttaagaaa gaagtccagg catgatggaa catgcccata catcccagca
     6601 cttgcggggc tgaggcagga ggatcaccag tttgaggtca gtgtggccta tacatatata
     6661 tcaaacaaaa ctagaccagt gtggcctata catatatatc aaacaaaact agaccagtgt
     6721 gtggaacaga ggaggacagg aatggagccc cggtggggac tgtggggaga ggcaggagga
     6781 gctggctgtc cggaggtcaa aggatctaga catctgggcc tgggctcaga tggggcacct
     6841 tgtgcacctc tgcctggaag catctggagt ggccgaggtg aacaggatga agtcatactg
     6901 tgccctcccc aggggaccag tggtgggtgc caatgagcta tgtggtaaga ccatgggggc
     6961 cataggacag gaaggcaggt tgggggctga ggagggcaga gctgggtttc tatccctttt
     7021 gtgagccgtt ttaattcgtg taaatgaggg tttttaattt gtcattctgg gttttagttc
     7081 tattaccgtc tcatttgcct ctaaacccta tttcgcctca gcctggaccg aattccattt
     7141 gatgcattag aactaattct cctcctaagg ggttagacgt tttctcccca cacaaaagtc
     7201 gcttattttg gataaagaga cgattagatt tatctttaat attttaatgg cagctgggat
     7261 tagcgggcaa gcagccccag ccagtgctct tctccccgga ttaggttctg ataaactctc
     7321 agatggggag cccccccccc ccgtactcca ctttctgttt tctacaaagt gcttttttat
     7381 tatttttctg tgggaaaaaa attcccttgt cttttggagc atcataaatt ccatttgctg
     7441 ttttctatta aatcaagttg attaaattcg ttgtgactct ttgcacatag atctttttaa
     7501 ttttaacgtc tccagcataa aatctttaat ggtgcaattt aaaattgctg caatatacag
     7561 tgcaggccct gatcaagcag atggagatga ggttctgccg ggtggggggg agtgaggaga
     7621 aggccgggat tgactcttcc ctgaagaact gagtgtgacc ctgagtaccc acactcaagc
     7681 gccttttcag ccacgctgtc gagcttcagg ctttccctag caccctggcc agaacttaca
     7741 gacaacttgc ctgtgcttct tactgtgtgt gtcctgctaa tactccttat aagaagggtt
     7801 ggtggctgaa ggtcacacag ccattgggtc caatttgtgt ggtctgcggc cagccccccc
     7861 acctcaactc tcagagcctc tatctatttc cttaccagtg aaatggggtg attggttctg
     7921 cacggactcc catggcttcg ataaagactt gatacagact gccttgtgaa cagtgcaggg
     7981 ctgggctttc ctggatatct gggggattct tccggtccta tttgggacca gacactctgt
     8041 catccgtccc aggaccggta gagctgaatt ttgaggtctt gggaattttc cctaactacc
     8101 aggtcttggc tgccccctcc ccttaactcc tmsccscccc cccccccccc cccgtttctt
     8161 tctcttgtcc ctccttctgt aatctgacct catcaagccc tgggaaggct gatccaccgg
     8221 agcaaatcct tcatccagag ggccaaaatc agcttaattt gtagcaattt gaagcaggtc
     8281 tttgccagga tttgaatatc aaattatccc atagcctaga caggcctggg agcttcccct
     8341 acccccatct ccccactgca atatggatgg atgtgttctg agatccatgg gggacactga
     8401 agagaccaga ctgggccaga ggatgctgat ggtatcaggt gtgctggggg aaaatggcac
     8461 cagtcagccc atcccaggcc tgagaaggag gacagggagg agacaggaac ttcctgattg
     8521 ggagactgat ccccttgtca aaccttcagg gaacatggac agatttttct tgtcatttaa
     8581 taatacagta taaagtaatg ataatataag catacagttt acaccatgct cacatcgtcg
     8641 tagttatcac tagaggagct gaagtggccc gagggacata catgaattac atgcaaatat
     8701 tctgccatct gtctgtttgg tttttttttt tttccaagac agggtttctc tgtgtaaccc
     8761 tggttatcct ggacctcact ctgtagacca ggttggcctc agaactcaca gagatcctcc
     8821 tgcctctgcc tcctgagtgt tcagattaag ttcctgtgcc accactgctc agctcaaaag
     8881 actattttga tgactaggaa agtctggaaa aatctcagct ggccaatcaa caaacacctt
     8941 gcccacctct ctccagcccc cggtgtctgg ggaagatccg accctcagtg ctacaagcca
     9001 ttttctttac agactgatcc atgctggccc tgcttgctca gactagccgc tacacctctt
     9061 tggcccttct ggttagtcct gtttcctctg gcaacctccc tcttgattcc tggtgtgtcc
     9121 tggggcctgg aagatgtcca cacccaccca ccacagggga tgcactgccc agacctgaga
     9181 accggagttg tacctgtctc atgggcctgt catgcctcgt gggatgtgaa agagtccttt
     9241 gtaagcctgt ctgtccaccc aatagcaaga ggagaaacca tccagatgtg gaggtacata
     9301 atttaatccc agcgcctggg aggcagagaa gggagggttt ctgtgagttc aaggacagcc
     9361 tggtctacat attaagctct aggctagcca ggggtataca atgagacttt gtcaaaaaaa
     9421 aaaaaaaaaa aaaaaaaaaa aaaagccggt ggcatgcatg tatatgtgta ctggcatgcg
     9481 tgtatatatg tactggcatg tgtgtaaacc ttgcgcttag gaggctgaac aggaggactg
     9541 tgagttcaag gacagcctag actatacact gagtctctgg ctaaacaaat actgtaaaca
     9601 cagcatctat cttgataaac aacccattaa acatacctat ggacacaaat aatatattca
     9661 ccaatttttt ttatatgcta ttcctgccag cacacagtag gcacttccta agggcctact
     9721 gtccctttca tctccagggc acaacattaa caattctgtt tgcccagcca cctccctttc
     9781 cactgtgacc cttgatcagc aagcacaata aatgtttgct aggtgactga tttataccag
     9841 ggatgaggga acgtctccaa gcttccacag aagtgaaaga caagtggcct ccatcgcccc
     9901 acaagggaag atgatgtcag gtgccagggt ggagatggtg aacatactcg gtgtgctgga
     9961 gtcccagcag ccaagctcac cctgtcccag ggtcaaaagt attcagctgc caggaccata
    10021 tcagaaatgt aatctcagta cccaaggtct gtcctaaggc ctggctcctt aggccttgca
    10081 cctcccacct aagttggaca gggccctttc ttccacttct ttagttggag atgggtgttt
    10141 aactcatatg atgagccatg tattgtatca gggctctttg tgagtggaga gggacacctg
    10201 agccctgggt acccaaaggt gtagagagat ggggacacag atgtagccag aatacctcag
    10261 cctccagcac ctggcatgtt gtagagcacc aattcacagt cttcaggggt gtactgaaag
    10321 ctggggactg tcaggagcct agaggtaccg actccatacc ctgagcagtt cttaggcgaa
    10381 gccgctgagt tgtgtgtggc tcgcctccac tgttacacca ctgaacacac tgactagtgt
    10441 ggaccaggca cttcccaagg agagtctcag aggtctggga aggccttgaa aaagtcttct
    10501 caatttttag aatggaaaag cagcccaaag aagtctaagt catttccagt gccacagagt
    10561 tcacatccgc tgggagcctc aactcccggc ctcctcttgg ctccactcct gcccaggatc
    10621 tcaggggtca ggggctgctc ttgagggttc ttcatggctc tccattccta ctgatccact
    10681 tccctaaagt cccaccggac tctgagctct acatctccct gtccactcac tcagttcctc
    10741 aggaggtcct ggagacccca gtagtgggga gagaggtcct tggtgcctct tagctgtccg
    10801 ctagctcaga gctgtaggga ggctgttgcg gtgcacagca ctgatcctaa ccaagtttgc
    10861 cagcccagct tttctcatgg ctcccacaca atggtctgct ttgtgcccac ctgccgataa
    10921 tgcttaatta ggcgaggagt tgattaattt gaggtaattg acagctaatt agaagcctac
    10981 aggctcatta acttgacatc ttgtctttgt gaattggctg ggtacggtca ggtgatggta
    11041 accgttgctc ccctccccag ctcttccaac cccaaagtct gaggcatcac ctaggggagg
    11101 gctgggcaga tccggggttc aggtagggca gtctcaagat caagagtaga gggaactggt
    11161 actgaagagt aaggagtccc gaatggagac gtctgctctg gctctaggga ggagagagaa
    11221 caagaatgaa gggcaaatgg acccgagaga gaactggacc ctagcaggag aatggggaag
    11281 cccgaagctc acatagtgag aaggggctgg ggagcaggtg agcctgttgg tactaggggt
    11341 aggctttgaa gagaggccga cttgtggtta aatcccagct ctgcctagag ctaggagact
    11401 ccaactgacc taaatgctct ctaagcccgg ggctcgtttg taaaacagga taacaatgtc
    11461 taccttacct catttggtta ctgtgaacag ttcatgggac acagaggcaa ggcctaggaa
    11521 gcacccagtt aaatgtcact gactgggctg gcaggatggg tcagtgggtg caaacttgat
    11581 gacccaagct tgatccctgg aaccggggtg gaaggagaga actgagtctg gaaggtccct
    11641 cccacctgca cacttgcact gtggcaaaca tcttcctgtg ctcacttaca catcacacac
    11701 actcactctc tctcacacac acacacacac aatgagtaaa acctaaagaa tgttcactat
    11761 ccttggcagt gggaagacgt gactgggaga tagtctaaga gggcaggttg tcagaggcaa
    11821 tgggaagccc ctcccttgga gaactagtgt cactgtggac atttgcattc acagcacaag
    11881 agtgtttgat ggtgtgggga tcaggtcttc ttgtcgcaac ctctttaggg ggcaatttag
    11941 tgatctatat tacaatgtat atgtagcttt tgacccaaga gttccctgct attaacgcac
    12001 tctgaggatt tacatgtatg cgcaaggatt tcattacagc gcagcctgca gaaccttcct
    12061 gcgtatccca gttggaaaga ctggaaatca cccaaaagtc catcagcgac taagttatgg
    12121 tacctccccg tagtacagta tattactatt gaatatatat gagcaggatc caaaatgcat
    12181 taaatagggg gaaaagccac gtatataaca gtgtatagtt cccacaagta gctcagtgga
    12241 tggaggtaca ggaaatggga ggcgtatttt tggcttgtac ttatcacctt ttcaaaaatc
    12301 agtttacaat taaccccccc accccccata agccctaaca atactgctat aatggcagag
    12361 tagcgccatg aacaagggcg ggaacacttg gtttcttctt ctacacagac aaacatggtg
    12421 gccattggcc acgtgtggcc attgaaccct tgaaatgtgg tctttttttt tttttttttt
    12481 ttttaagagg atggagaggt ggttcagtgg ttagaagtac gtactgctct tgtgaatttg
    12541 gttctttttt ttatttattt atttatttat gtaaattctc tttgacatgg tggtaagaac
    12601 ctcactgcag gtctgggtat ggaggtctgg gtgtcgtacc tcagtcaccg tggtaccgca
    12661 gtcaccatgc tgcaggccat gcaaggagtg agggctggag gtaaggaaac agagcgggct
    12721 tccaggaagg tcagagctcc agtacttcag actctggctc cagggatgga gcctaggctg
    12781 tgccagtgca gttctgctct gctccacagc ctgtgccttt tgggctttgg gaaacctctc
    12841 acgagttgct ttgtttagag accgtgttcc ccatccctcc tccctgcatc tgtcccctgg
    12901 ttagagttgt atgaagcgga agacaatctg gggcttatta gtgtcccagg gagacatgta
    12961 actgtgctgc tgggacccaa acagcaagag tcttagcagc aaactccagc cgagaaagga
    13021 ctgagggatt gtctcaccta attacaggga atggctctct ttgtatttgt gattcggtac
    13081 ggttgtcaga tcgcttttga tcgcttttct ccatttgctg gctcaggcca tgctggaatt
    13141 gtgctaatta gaggagaaat ggacagagtg atcctgtcaa ccctggttac tggtggggga
    13201 caaagcagaa tattattcta aggcaatgtt ttgtaagagc ccttctcctg ctctcattag
    13261 gaggaagagg ccccaggagg ggaagttccg aatgcttggc ctggctaatg gagcattggg
    13321 gtgttcttgt gctaattaac aggctctgcc agcactccag ccaaccaggg gactcgcagt
    13381 tgcacttggt atctcctgaa gtctccaact gtccctggtc taaaacaaaa tctaccagac
    13441 aaaaggtggg aaggtcaatg gccttttcag acataggggc acacttccaa agtggcaaag
    13501 gtctaaacag atatgaacag ccagttagac ctagtgtcga cccaggtggc acttttcggg
    13561 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    13621 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    13681 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    13741 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    13801 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    13861 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
    13921 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    13981 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    14041 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    14101 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    14161 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    14221 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    14281 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    14341 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    14401 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    14461 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    14521 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    14581 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    14641 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    14701 cttc
//
