LOCUS       sequence-446999-       10901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Honnell V, Sweeney S, Norrie J, Parks M, Ramirez C, Jannu AJ, Xu B, 
            Teubner B, Lee AY, Bell C, Dyer MA
  TITLE     Evolutionary conservation of VSX2 super-enhancer modules in retinal 
            development.
  JOURNAL   Development. 2024 Jul 1;151(13):dev202435. doi: 10.1242/dev.202435. 
            Epub 2024 Jul 12.
  PUBMED    38994775
REFERENCE   2  (bases 1 to 10901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2024-07-1"; volume: "151(13):dev202435. doi";
            pages: " 10.1242/dev.202435. Epub 2024 Jul 12"
COMMENT     Sequence Label: Vsx2 hR3-16 +SV40 base prom-GFP-IRES-AP
FEATURES             Location/Qualifiers
     source          1..10901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    828..962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(852..871)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     906..925
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(968..1163)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      982..1117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    1222..1243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1258..1263,1264..1281,1282..1288)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1296..1312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1301..1323
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1320..1336
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1320..1336
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1679..1698
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1697..1752
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1798..1817
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(2312..3811)
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="LLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPA
                     QTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKH
                     VPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVT
                     TTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKY
                     MFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHL
                     MGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRA
                     YRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKAR
                     DRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFAR
                     GPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     misc_feature    3823..4375
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3825..4398
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3985..4004)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4214..4231
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(4428..5141,5142..5144,5145..5147))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4431..5141,5142..5144,5145..5147))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4473..4494)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4822..4841
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5081..5102
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5144..5153
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5188..5384)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5202..5337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9766..9870
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9871..9939,9940..10731)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10089..10108)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga
      841 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      901 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      961 ttagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     1021 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     1081 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     1141 tggttgctga ctaattgaga tgcggatccg ctggcacgac aggtttcccg actggaaagc
     1201 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     1261 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     1321 aggaaacagc tatgaccatg attacgccaa gcttgggctg caggtcgagg gatctccata
     1381 agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat ctggctagta
     1441 aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa caaaatataa
     1501 aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca gcagggggct
     1561 gtttcatata ctgatgacct ctttatagcc aacctttgtt catggcagcc agcatatggg
     1621 catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga tttgccctcc
     1681 catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca atgaaaataa
     1741 atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc ccataatttt
     1801 tggcagaggg aaaaagatct cagtggtatt tgtgagccag ggcattggcc acaccagcca
     1861 ccaccttctg ataggcagcc tgcacctgag gagtgcggcc ggccgcgata tcctcgaccc
     1921 cactgggctg taggtggtgg ctggatggcc tgagccccct cctttcctgg ggtgcaggca
     1981 gccacagggc acaacctgca gtccctgggg ttggggtagg gagtccgaga gcagccaagg
     2041 ggcaggtttg gtgtcatggg ctcagtcccc agagcgcacg gggaggggaa ggttccatgg
     2101 caggacctct gtatagggac tccggggatg acgactcggg ctggaggcca ggcaggacga
     2161 caggaggtgg ggagcaggag aactccggga tggggaagca ggagccccag ggacgggaca
     2221 ctcagggagc agtggccgtc tccagcagca gcagggtccc ggccagcaga ggaagcaacg
     2281 cggggaccac ggaccgcccc gggtgcgcgg cgtcggtggt gccggcgggg ggcgccaggt
     2341 cgcaggcggt gtagggctcc aggcaggcgg cgaaggccat gacgtgcgct atgaaggtct
     2401 gctcctgcac gccgtgaacc aggtgcgcct gcgggccgcg cgcgaacacc gccacgtcct
     2461 cgcctgcgtg ggtctcttcg tccaggggca ctgctgactg ctgccgatac tcggggctcc
     2521 cgctctcgct ctcggtaaca tccggccggg cgccgtcctt gagcacatag cctggaccgt
     2581 ttccgtatag gaggaccgtg taggccttcc tgtcccgggc cttgccaggg gccagcccga
     2641 agatggagct ccctcgcagg gggtagcctc cgaaggagaa gacgtgggag tggtcggcag
     2701 tgacgaggct cagcgtgtcc tcctcgctgg tgagctggcc cgccctctca atggcgtcgt
     2761 cgaacatgat cgtctcagtc agtgcccggt aagccctgct ttcatgatga ccatggtcga
     2821 tgcgaccacc ctccacgaag aggaagaagc cgcgggggtt cctgctcagc aggcgcaggg
     2881 cagcctctgt catctccatc agggaggggt ccagtgtgga gtctcggtgg atctcgtatt
     2941 tcatgtctcc aggctcaaag agacccatga gatgggtcac agacgggtcc agggaagcct
     3001 gcatgagctc agtgcggttc cacacatacc gggcaccctg gcgcttcgcc agccattcct
     3061 gcaccagatt cttcccgtcc agcctggtcc caccttggct gtagtcatct gggtactcag
     3121 ggtctggggt tcccatgcga aacatgtact ttcggcctcc acctaggatc acgtcaatgt
     3181 ccatgttgga gatgagctgc gtagcgatgt cctggcaccc ctcctggcgg gccgaggcag
     3241 gcacgtcggc gtccgagtac cagttgcggt tcaccgtgtg ggcgtaggtg ccggctggcg
     3301 aggcgtgctg cactcgtgtg gtggttacca ctcccactga cttccctgct ttcttggccc
     3361 gattcatcac ggagatgacc tcgttgccgc gtgtcgtgtt gcactggtta aagcgggcgg
     3421 ctgcactcaa gccaatggtc tggaagttgc ccttgacccc gcacaggtag gccgtggctg
     3481 tggctccact gtctggcaca tgtttgtcta cattgtatgt cttggacaga gccacatatg
     3541 ggaagcggtc catggccagg ggtatctcag gccccagttt gtccttcttc tgccctttta
     3601 ggatcctggc agctgtcacc gtagacaccc ccatcccatc gcccaggaag atgatgaggt
     3661 tcttggcggc tgtctgtgca ggctgcagct tcttggcggc acccagggcc tcggctgcct
     3721 cgcggttcca gaagtccggg ttctcctcct caactgggat gatgcccagg gagagctgta
     3781 gcctcaggcc cagcagcagc agcagcagca gcctcgaccc catattatca tcgtgttttt
     3841 caaaggaaaa ccacgtcccc gtggttcggg gggcctagac gtttttttaa cctcgactaa
     3901 acacatgtaa agcatgtgca ccgaggcccc agatcagatc ccatacaatg gggtaccttc
     3961 tgggcatcct tcagcccctt gttgaatacg cttgaggaga gccatttgac tctttccaca
     4021 actatccaac tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt atcttataca
     4081 cgtggctttt ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg
     4141 gtcgctacag acgttgtttg tcttcaagaa gcttccagag gaactgcttc cttcacgaca
     4201 ttcaacagac cttgcattcc tttggcgaga ggggaaagac ccctaggaat gctcgtcaag
     4261 aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat ggtggaaaat
     4321 aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt aacgttaggg
     4381 gggggggagg gagagggggg gggggggggg gggcggaatt ggccgcttta cttgtacagc
     4441 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
     4501 cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc
     4561 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
     4621 ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg
     4681 tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg
     4741 ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc
     4801 tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc
     4861 tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg
     4921 gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc
     4981 acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg
     5041 ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc
     5101 aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt
     5161 ggatcccggg cccgcggtac caagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     5221 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     5281 aattagtcag cgatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     5341 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcagatcgc agatctcgag
     5401 agggaaaact tcctagagga ggcagcagtg gggctggact atgaagctag aatgatcact
     5461 agcctgcagc agtgaggccc cagctccttt gcctgtgacc atctcctccc aacgcttttc
     5521 caccaaatct caggcttagg acacccctct ggaaaaatcc caccctgccc accatctcct
     5581 catgttttct ttttcttttc ttttttcttt tcttttttct tttcttttct ttttcttttc
     5641 tttctttctt cctttcttcc ttcctttttt tttttttttt gatgttttgc tcttgtcacc
     5701 ctggttagag tccagtgatg cactctcggc tcactgcaac ctccgcctcc cgggttcaag
     5761 tgattctcct acctcagcct ccaagtagct gcgattacag gcacgtgcca ccaggcccag
     5821 ttaatttttg tatttttagt agagacaagg cttcaccatg ttggccaggc tggtcttgaa
     5881 tttctgacct caggtgatct gcctgcctcg gcctcccaaa gtgttgggat tacaggcgtg
     5941 agccactgcg cctggccatc ctcgtgtttt cttttagcca tcctgggaag aagttgggaa
     6001 gaaggtcacc cactctcaca cggccacagc ccaattttct cttgaaaatc gctactagaa
     6061 tcaccatttg tcatagtcca agggtcgggg acagagtctg ggggaataat ttgacgaagg
     6121 tgggcaggaa acccccctcc ctcccgtcga gccctgctgg agggaaggac tgtggtaggc
     6181 gcagaggtga ggaggccaac cgaatgcaga aacccagcca gggaaaggtt tagccccaca
     6241 atccaattcc acttaatatc ccataataga atgctgtcaa catgagtttc ctgttcaagt
     6301 ggatttgtta ggaaggctgg ggaggtcaga ggtgtattcc ggccctcctg gtgtggggac
     6361 tccagtaggg tgtgtgtggt gggggttact tgggcacaag ggccccctcc ccaccctctc
     6421 ccctcagcaa gccaaacagg ctctcaggaa ggggtttgtc acacttgtct gtaccaagac
     6481 ctcatgagtt tgtgatggct tgcagagggt cctggagcct cctgagggag cgtgcatctc
     6541 ctttgagaag cgtccagcca ctaaggcaga gagcaggata ttcatgtgaa gggattcaga
     6601 ccttgagcag aaggtgggga ggggttccca gttcggggga aggtacacag gaggagccac
     6661 agggccaaac ttgaggcaac cttggggggc aaggggccac ttagggagaa ccctgaagat
     6721 ctgggttggc ctcagccccc atgagtgaca acccagtggg tgaccccacg tagttactca
     6781 aactggctgc tgcctgctcc ttctgccccc ctggctggcc cattgctagg cgtccttcct
     6841 cccagctgac taagaatcct tttgggggag acttctgtga gagcttgtga tcgagcggtc
     6901 cccgtggatc agacacagtg ctgggccttc catatgctgc tgcctcatca aattctgtgg
     6961 agccaggcca gggagcagca gcaggggtca aggctgggga gaagggctgt gtgagcacag
     7021 gagccgttat tatcctcatt ttccccaagt aataggggct caggggtttt aagaaacaaa
     7081 actagggcca ggcgtggtcg cttacacctc tagcctctaa tcccagcact ctaggaggct
     7141 gaggccagtg gatcacttga gcccaggagt ttgagactag cctgggcaac atagcaagat
     7201 cccatctcta ccaaaaaaaa aaaaaaatag aaaggcatgg tggtgcacac ctgtagtgcc
     7261 agctactcag gaggttgagg caggaagatc acttaagccc aggagtttga ggcttcagtg
     7321 agctgtgatt gagtcactgc actccaacct gggcaacaga gtgaggccct gtctctaaaa
     7381 aatatatttt aaataaataa atagaaacaa atcaagacct cacatagtag gggagggcac
     7441 tgggacaagc atgaagttct ttctgacccc aaagctggtg caggagagat ggggagatga
     7501 atgaagactc agtgagagcc ttcagaaacc cagcctgcgc ctggtcttgg agagcggatg
     7561 ttgggtgcat ttttctgtaa gtatccagga gggtgtgggg cagaagttgc aaggatgagg
     7621 tcaggccttg gctcctctgg ggcctgcggg tgggtgagac caggggaggg gggaatgggc
     7681 ctggggccga ggagggcagg gctgggcctc cggctctttt gtgagctgtt ttaattcgtg
     7741 taaatgaggg tttttaattt gttattctgg gttttatttc tattaccatc tcatttgcct
     7801 gtaaacccta ttctgcccct gcctggaccg aattccattt gatgcattag gactaattct
     7861 cctcccaaag ggttagatgt tttctcccca cacaaaagtc gcttattttg gataaagaga
     7921 cgattagatt tatctttaat attttaatgg cagctgggat tagtgggcaa acagccccga
     7981 ctggggttgt tcacggcgga ttaagttctg atcaactctc agatggggag cccccacccc
     8041 caccccactc cactttctgt tttccacaaa gtgcttttta ttattttttg ttgaaaaaaa
     8101 aattcccttg tcttttggag catcataaat tccatttgct gttttctatt aaatcaagtt
     8161 gattaaattc gttgtgactc tttgcacata gatcttttta attttaacgt ctccagcata
     8221 aaatctttaa tggtgccatt tgaaattgct gcaatataca gtacaggccc tgagaaaaca
     8281 gatggaaatg aggttctgcc agggatgggg gaggtcctat caggaggggt acagttggga
     8341 gaaggcacag acctccaggt gaaggcaggg gactgactct tcctgaggtc cagagagccc
     8401 cacgttctcc attcagctat tttctagctt cagggctttg gctaggaact tggcccatgc
     8461 tatggagggc ttgctatgtg cctggcactg tgtatgcatc gccagtcatc cttacaagga
     8521 ggctcagaga gaggtcactt gctcaaggtc acgtagcaag tcggtggcag tggttgagtc
     8581 cagattgtct ttgccaataa agcctcatct cccctttcat cagtaaaatg ggggtgatcg
     8641 gttccgccct gggtcacagg tgcttggaaa gattggatga ggtccacaaa ctgctttggg
     8701 aacagtacag agctgtgctc tcctgggggg atggaggagt ggggctgacc catttgggcc
     8761 tcagaggctc tgctatcact gggtagagct gccttttgag accctgggaa ccttcactaa
     8821 ctccagggtc tcggcttccc cccgcccctc agctctccag ctctccggcc tccggcctca
     8881 ggctttttct ccccgtctct aatcctgacc tcatcaagtc ctgggaagag ggttgatcca
     8941 caggagcaaa tccctcattc agaggggcaa aatcagctta atttgcagca atttgaagca
     9001 ggtctttgcc aggatttgaa tatcaaatta tcccacagcc tgggctggtc tgggaggcct
     9061 cccccggctg caatatggac cgatgtgctc aggcttcacc agggagattg tcctgggagt
     9121 gactgaagct gaggctgtgg agatgggagt gagtgtcaga gggtagtggg caaggtgggg
     9181 gtcaccagtc agcccccact gccagagggc actgcagcag tgactgaggg acagatggag
     9241 tttggggtca aaacctcctg ttcttgagac tcaccggggg ctcttgtcaa atgtgcggat
     9301 tcccaggccc ttctacaaac ataagaaatc aagattttct tgactaagaa agtttagaaa
     9361 aatccggtgc agctcagtta gccaatcaac aaacacttgc tgtgcacaat ctgtactgcc
     9421 tctcattttg agagaatcag cctcctctgg tgcccaccct gttcattccc agcctctggt
     9481 actgtggaca atatttcctt ggctgtctgc tccatgatgg cccctgaggc ctgagtcagc
     9541 ggctgcagag agctcttcag ccttcccttc ctggtctgca tgcccaactc ctctggccac
     9601 ccggcccttt cattcctttt ctgcaagctc aggcctagca gacatcctct tatgtcagtc
     9661 acacaggcct cgctgcccag acctgagccc cggggctcag acccctctca tgggcatgtc
     9721 atgagctgtg aggtcgaccc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     9781 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     9841 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     9901 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     9961 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
    10021 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
    10081 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
    10141 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
    10201 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
    10261 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
    10321 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
    10381 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
    10441 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
    10501 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
    10561 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
    10621 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    10681 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
    10741 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    10801 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    10861 cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
