LOCUS       sequence-447043-        2450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for nuclear targeting of domains (C-term NLS, 
            myc tags).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR4-myc-nuc (w556-1)
FEATURES             Location/Qualifiers
     source          1..2450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      757..800
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      932..959
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     967..990
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    1009..1108
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1168..1188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1192..1212
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1216..1236
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1249..1278
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(1330..1429)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1541..1560)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1552..2361
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1619..1638)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgctagc atggatctcg gggacgtcta
      721 actactaagc gagagtaggg aactgccagg catcaaataa aacgaaaggc ccagtcttcc
      781 gactgagcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
      841 ccgccgggag cggatttgaa cgttgtgaag caacggcccg gagggtggcg ggcaggacgc
      901 ccgccataaa ctgccaggca tcaaactaag cagaaggcca tcctgacgga tggccttttt
      961 gcgtttctac aaactcttcc tgttagttag ttacttaagc tcgggcccca aataatgatt
     1021 ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg cttttttata
     1081 atgccaactt tgtacaaaaa agcaggctcc accatggccc aggtgcagct gcaggtcgac
     1141 ctcgagatca aacgggcggc cgcagatcca aaaaagaaga gaaaggtaga tccaaaaaag
     1201 aagagaaagg tagatccaaa aaagaagaga aaggtagata cggccgcaga acaaaaactc
     1261 atctcagaag aggatctgaa tggggccgca tagtctagaa gctcgctgat ccggtaccga
     1321 attatctaga cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
     1381 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
     1441 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1501 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1561 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1621 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     1681 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1741 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     1801 cattttatcc gtactcctgg tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     1861 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     1921 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     1981 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     2041 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2101 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2161 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2221 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2281 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2341 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacatta ttcagattgg
     2401 gccccgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
