LOCUS       sequence-447050-        3057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This is the native promoter from the aac(3)-Ia gentamicin resistance
            cassette in IDT backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3057)
  TITLE     Cultivarium plasmids
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGLU_65
FEATURES             Location/Qualifiers
     source          1..3057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(552..557,558..574,575..580)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     complement(883..899)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(883..899)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(896..918)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    907..923
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(931..937,938..955,956..961))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    976..997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1114..1131)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1285..1873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1365..1384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2044..2859)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2767..2786
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(2860..2962)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3030..3048
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg agaacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttaaga cgggcgacag
      481 atgtgactca ggtctcacgt agggtagtcg tgaggtgaac tgttccgcag ccacacgccg
      541 tgtgaagctg gttgacataa gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc
      601 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
      661 ttttcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca tccaagcagc
      721 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgaa tgtgagaccg
      781 ggattcacat tggatacgag atccgtagat tgataagagg acacggaata tcccatcagt
      841 tctggaccag cgagctgtgc tgcgactcgt ggcgtaatca tggtcatagc tgtttcctgt
      901 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      961 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     1021 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     1081 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1141 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1201 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1261 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1321 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1381 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgtcgctta ccggatacct
     1441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1621 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1681 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1741 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1801 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1861 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1921 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1981 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2041 cagttagaaa aactcatcga gcatcaagtg aaactgcaat ttattcatat caggattatc
     2101 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     2161 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     2221 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     2281 gactgaatcc ggtgagaatg gcaagagttt atgcatttct ttccagactt gttcaacagg
     2341 ccagccatta cgctcgtcat caaaatcact cgcaccaacc aaaccgttat tcattcgtga
     2401 ttgcgcctga gccagacgaa atacgcgatc gccgttaaaa ggacaattac aaacaggaat
     2461 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     2521 atattcttct aatacctgga atgctgtttt ccctgggatc gcagtggtga gtaaccatgc
     2581 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     2641 gtttagcctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     2701 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     2761 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     2821 cggcttcaag caagacgttt cccgttgaat atggctcatt ttagcttcct tagctcctga
     2881 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     2941 ggaacctctt acgtgccgat cacacatttc cccgaaaagt gccacctgac gtctaagaaa
     3001 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc ttttgtc
//
