LOCUS       sequence-447103-        4746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible 5' entry clone containing the Human GLAST 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: p5E_hs_Glast (JDW 1182)
FEATURES             Location/Qualifiers
     source          1..4746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    2792..2915
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2987..3006)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2988..3006)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3011..3027)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3011..3027)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3129..3148)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3140..3949
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3207..3226)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4096..4684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4585..4604
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgta tcgataggta ccatgtctac acaaactgaa
      721 ataatgaaat ggtttttaac caagctttag ggattagaga ggaaaaaaaa agtgaaaaag
      781 aaagggcacc agggtcttcc catctctatg ctaaccaaag tgtggttttc cagccctttt
      841 tcttgcctat taacaaaaat aaaaacaaaa aatggaaatg ccactgggct tgaaacccca
      901 tttaaaatca gatttctctt tcgagttccc ttgcaataca agtcaaagtc atttccacca
      961 aaaaaaaaaa gcggtggggg gggtgcgcaa attaatccta aactcatggc tcagatagga
     1021 cctggtatag taaataaatg cttttaaaag tagtcatggc attgagaaat ggctccatgc
     1081 acctaaacca gaggcaccaa tgtaaaaact agtaattgtg ttttaacaca aaagattgaa
     1141 aagcgttttg tttcacatcc taatattatt ccacccactt aaaaagagtt acttaatatt
     1201 gactctgtat taggtataag acaagttctt gccctcaaag aacttataat ttagttgaga
     1261 aaccaaaact taacatgagc ttaaaaagaa ggatgcagta caaaacatgg tataattaag
     1321 tgctaaacaa tgtagtatag atgctcagtg acctaggaga tcagaggaag ggaagggtca
     1381 ctgagggctg gggatgtcaa aagcatgtac tttcagtaag ttcttaaaat gatagcatgt
     1441 tagaaagctg ttataattgc tgctgatttg taattttaaa gcgttgacat gattcaccat
     1501 tgctttccaa ccagtagctg tttaaaagga catacagttg agggtgaaag gagatatttc
     1561 tttatgatct ccagagattt gtattgaggg gaaaaaagac atactttctt gttgaataat
     1621 agaccaagaa ttgagcatat tttgcatggg gaatattaaa ttcagtgaac aagaatccaa
     1681 acctcaggct gtgattctgg tataaatttt tgtttctaat gaaccatttt cttccttcta
     1741 aagaagattt aagttttggg aataagtcac ctcaaaaaat ttttttttta ttttctaata
     1801 tctattttca caatttattt ttcttttaag aaaagtggac tagaagctca agttaccttc
     1861 tcaaggcaat gagaattttt tagctctaaa aaagttgtgt gtggtaggga gaacatttag
     1921 catcctttaa aatggagaca tctgaacagc aacctatctt ttgaatcgcc aaatctcaag
     1981 tgtactttgc actctcggtc ttaaaatgct cttcattttc tagtttggca aaaattccag
     2041 tctctttaca tttgctacct tttccgtctt tgcaacacag gcagtgtgag ctaaattagg
     2101 tttgttaaaa tgaagttttg aagtttcaac tgctgaaata gaggcatgtc cctaacttta
     2161 gacaaattta gagaatcgta gtaagcatta ttttttttct acatacttgt ctgcattcag
     2221 tgtcttccca agaaacgtca gttaattccc cgattcgaaa aaggaacttt ctcgtaacag
     2281 ttgtacaacc aggctaaaga gcacaccctc gtcttccctg aaagggagga aacatgcaat
     2341 aatgtgaggt aattaaattt agccttagaa aagtctttga aacgttctaa tcaagacttt
     2401 cagctttccg attgtgtgcg ctttattttt caattcttcc tagtcccggt tttgcatttc
     2461 taatgacagt gaactgacaa agcgtgaaag tggtctaagg agcaaaacaa agccagcaag
     2521 cctgcttctg gtgtgccaag attaatcaat attccagaaa cctcggggtt tccccctcct
     2581 ccctgtgcgg tttccattcc ccactcctcc tttcccgggc acagactcct ccccttttct
     2641 taagttgccc tgatagtaac ttgcagtttc agagcacatg cacactgtca gggctagcct
     2701 ggctgcttac gcgcgctgcg gattgttgct ccgttgtacc tgctggggaa ttcacctcgt
     2761 tactgcttga tgggctcgag atccaccggt tcaagtttgt acaaaaaagt tgaacgagaa
     2821 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     2881 taatactgta aaacacaaca tatgcagtca ctatgaacca actacttaga tggtattagt
     2941 gacctgtaga attcgagctc tagagctgca gggcggccgc gatatcccct atagtgagtc
     3001 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     3061 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     3121 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     3181 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     3241 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     3301 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     3361 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     3421 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     3481 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     3541 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     3601 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     3661 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     3721 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3781 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3841 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     3901 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     3961 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     4021 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     4081 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4141 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4201 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4261 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4321 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4381 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4441 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4501 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4561 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4621 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4681 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4741 catgtt
//
