LOCUS       sequence-447104-        3981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible 5' entry clone containing the Zebrafish unc-503 
            promoter with a minimal c-fos promoter and b-globin intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: p5E-dr_Unc (JDW 1365)
FEATURES             Location/Qualifiers
     source          1..3981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          1361..1933
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     protein_bind    2027..2150
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2222..2241)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2223..2241)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2246..2262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2246..2262)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2364..2383)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2375..3184
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2442..2461)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3331..3919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3820..3839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgtc tagaggtacc cctcgagcca ttatcgtctc
      721 cctccagcac caaccagaaa gttgcttaat ggtttgttac agtatgtgtg taacaccttc
      781 ccccccagcc cagctctcca cttttctcat tctagaaagt accagtcaac tctccaccag
      841 cccagctgtt ggcagacgca cacctccatc cccctgccct cagacatttg ccactgattt
      901 ctcagctgtc atcccctctc cataaataga ccctatcaga gaaagtccat tgcactaata
      961 taaggggtga ccacatttct acaaaaccac aattaatgta caatcacagg aataaacaaa
     1021 cactgcctaa acacacaata tcaagaacac tcaattacaa tctcaggttg tcattcgcaa
     1081 gctgcgtagt tcattcacaa tgaacagggg cttaaaacaa aagtcattcc acccctcacc
     1141 atgaaatgga ttcatccgta atcctggtat gttaacgctc tatgtgtaag atatatataa
     1201 gagtgactct actagtgata tcgtcgaccc agtgacgtag gaagtccatc cattcacagc
     1261 gcttctataa aggcgccagc tgaggcgcct actactccaa ccgcgactgc agcgagcaac
     1321 tgagctcgga tccgtttaaa ccgatcctga gaacttcagg gtgagtttgg ggacccttga
     1381 ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag
     1441 ggtgttgttt agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg
     1501 tttctttcac tttctactct gttgacaacc attgtctcct cttattttct tttcattttc
     1561 tgtaactttt tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt
     1621 ttatttgtca gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat
     1681 attatattgt acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag
     1741 aatatttctg catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta
     1801 caccctggtc atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat
     1861 gaggataaaa tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt
     1921 ctctttccta cagctcctgg gcaacgtgct ggttgttgtg ctgtctcatc attttggcaa
     1981 agaattcctc gacaccggta tcgatacgcg ttacgtagcg gccgctcaag tttgtacaaa
     2041 aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     2101 aaaaaacaga ctacataata ctgtaaaaca caacatatgc agtcactatg aaccaactac
     2161 ttagatggta ttagtgacct gtagaattcg agctctagag ctgcagggcg gccgcgatat
     2221 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     2281 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     2341 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     2401 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     2461 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     2521 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     2581 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     2641 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     2701 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     2761 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     2821 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     2881 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     2941 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     3001 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     3061 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     3121 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     3181 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
     3241 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     3301 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3361 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3421 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3481 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3541 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3601 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3661 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3721 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3781 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3841 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3901 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3961 ctggcctttt gctcacatgt t
//
