LOCUS       sequence-447107-       10453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike G142D, V143-Y145 deletion for pseudovirus 
            production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 G142D
            (GAT)+d...
FEATURES             Location/Qualifiers
     source          1..10453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    198..786
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(251..271)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(669..680)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    839..960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(850..869)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     904..923
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      1075..1121
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1151..1287
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    1332..1403
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        1448..1644
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1495..1630
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1658..2452
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1712..1731)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2322..2341
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2463..2687
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2883..3471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2963..2982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(4217..4285,4286..5077)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4435..4454)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      5090..5226
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5378..5449
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(5454..5500)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    5518..5566
                     /note="synthetic polyadenylation signal"
     enhancer        5647..5950
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5952..6154
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6105..6125
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          6288..6763
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 gtacatttgg ttgggcttta tcgccggact tatagctatc gttatggtca caattatgtt
       61 gtgctgtatg actagctgtt gttcctgctt gaaaggatgt tgcagttgcg gatcttgttg
      121 taaataaagg atcctctaga agttgtctcc tcctgcactg actgactgat acaatcgatt
      181 tctggatccg caggcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
      241 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
      301 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
      361 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
      421 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
      481 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
      541 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
      601 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
      661 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
      721 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
      781 ccccgcctgt ctagcttgac tgactgagat acagcgtacc ttcagctcac agacatgata
      841 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      901 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      961 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     1021 taaagcaagt aaaacctcta caaatgtggt attggcccat ctctatcggt atcgtagcat
     1081 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gtgcccctcg ggccggattg
     1141 ctatctaccg gcattggcgc agaaaaaaat gcctgatgcg acgctgcgcg tcttatactc
     1201 ccacatatgc cagattcagc aacggatacg gcttccccaa cttgcccact tccatacgtg
     1261 tcctccttac cagaaattta tccttaaggt cgtcagctat cctgcaggcg atctctcgat
     1321 ttcgatcaag acattccttt aatggtcttt tctggacacc actaggggtc agaagtagtt
     1381 catcaaactt tcttccctcc ctaatctcat tggttacctt gggctatcga aacttaatta
     1441 agcgatctgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     1501 cccctaactc cgcccagttc cgcccattct ccgccccatc gctgactaat tttttttatt
     1561 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     1621 tttggaggcc taggcttttg caaaggaggt agccaacatg attgaacaag atggattgca
     1681 cgcaggttct cccgccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     1741 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     1801 tgtcaagacc gacctgtccg gtgccctgaa tgaactccag gacgaggcag cgcggctatc
     1861 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     1921 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     1981 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     2041 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     2101 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     2161 cgaactgttc gccaggctca aggcgcggat gcccgacggc gaggatctcg tcgtgaccca
     2221 cggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     2281 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     2341 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     2401 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct agtatgtaag
     2461 ccctgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     2521 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     2581 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     2641 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggtta attaaccagt
     2701 caagtcagct acttggcgag atcgacttgt ctgggtttcg actacgctca gaattgcgtc
     2761 agtcaagttc gatctggtcc ttgctattgc acccgttctc cgattacgag tttcatttaa
     2821 atcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2881 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2941 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3001 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3061 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3121 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3181 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3241 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3301 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3361 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3421 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3481 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3541 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3601 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3661 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcatt taaatttccg
     3721 aactctccaa ggccctcgtc ggaaaatctt caaacctttc gtccgatcca tcttgcaggc
     3781 tacctctcga acgaactatc gcaagtctct tggccggcct tgcgccttgg ctattgcttg
     3841 gcagcgccta tcgccaggta ttactccaat cccgaatatc cgagatcggg atcacccgag
     3901 agaagttcaa cctacatcct caatcccgat ctatccgaga tccgaggaat atcgaaatcg
     3961 gggcgcgcct ggtgtaccga gaacgatcct ctcagtgcga gtctcgacga tccatatcgt
     4021 tgcttggcag tcagccagtc ggaatccagc ttgggaccca ggaagtccaa tcgtcagata
     4081 ttgtactcaa gcctggtcac ggcagcgtac cgatctgttt aaacctagat attgatagtc
     4141 tgatcggtca acgtataatc gagtcctagc ttttgcaaac atctatcaag agacaggatc
     4201 agcaggaggc tttcgcatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4261 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4321 agatcagttg ggtgcgcgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4381 tgagagtttt cgccccgaag aacgctttcc aatgatgagc acttttaaag ttctgctatg
     4441 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4501 ttctcagaat gacttggttg agtattcacc agtcacagaa aagcatctta cggatggcat
     4561 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4621 acttctgaca acgattggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4681 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4741 gcgtgacacc acgatgcctg tagcaatggc aacaaccttg cgtaaactat taactggcga
     4801 actacttact ctagcttccc ggcaacagtt gatagactgg atggaggcgg ataaagttgc
     4861 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4921 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4981 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5041 cgctgagata ggtgcctcac tgattaagca ttggtaaccg attctaggtg cattggcgca
     5101 gaaaaaaatg cctgatgcga cgctgcgcgt cttatactcc cacatatgcc agattcagca
     5161 acggatacgg cttccccaac ttgcccactt ccatacgtgt cctccttacc agaaatttat
     5221 ccttaagatc ccgaatcgtt taaactcgac tctggctcta tcgaatctcc gtcgtttcga
     5281 gcttacgcga acagccgtgg cgctcatttg ctcgtcgggc atcgaatctc gtcagctatc
     5341 gtcagcttac ctttttggca gcgatcgcgg ctcccgacat cttggaccat tagctccaca
     5401 ggtatcttct tccctctagt ggtcataaca gcagcttcag ctacctctca attcaaaaaa
     5461 cccctcaaga cccgtttaga ggccccaagg ggttatgcta tcaatcgttg cgttacacac
     5521 acaaaaaacc aacacacatc catcttcgat ggatagcgat tttattatct aactgctgat
     5581 cgagtgtagc cagatctagt aatcaattac ggggtcatta gttcatagcc catatatgga
     5641 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     5701 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     5761 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     5821 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     5881 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     5941 tattaccatg ctgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     6001 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     6061 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     6121 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatcgcctg
     6181 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc
     6241 ctcgaagctt acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg
     6301 acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga
     6361 gaagtaacag ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa
     6421 aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc
     6481 tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa
     6541 taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc
     6601 atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta
     6661 ccattctgct tttattttat ggttgggata aggctggatt attctgagtc caagctaggc
     6721 ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct
     6781 ggtctgtgtg ctggcccatc actttggcaa aggcggccgc catgtttgtg ttcctcgttc
     6841 tcctgcccct cgtttcttcc caatgcgtga acctcacaac tagaacgcag ctcccacccg
     6901 cttacacgaa ctcttttact cgtggagtat actatcccga taaagtcttt aggtccagtg
     6961 tccttcattc tactcaagac cttttcctgc cattcttctc aaatgtcacc tggttccatg
     7021 ctatccacgt ttctggcacc aacggaacta aacgatttga taatcccgtc ttgccattta
     7081 acgatggcgt atactttgcc tcaacggaga agtccaacat tatccgggga tggatatttg
     7141 gcaccaccct ggactccaag acacagtctc tcctcattgt gaacaacgcc accaatgtcg
     7201 tcatcaaagt ctgtgaattt caattttgta acgatccctt tctcgatcat aagaataaca
     7261 agtcttggat ggagtcagag ttccgcgttt acagttccgc taacaactgt acttttgagt
     7321 acgtttccca accatttctc atggatcttg aagggaagca aggcaacttc aagaatctcc
     7381 gcgaatttgt gtttaagaat atagacggct acttcaaaat ttatagcaag cataccccga
     7441 taaatctggt tcgggatctc cctcaaggct tctccgcgtt ggaacccctc gtggacttgc
     7501 cgattggcat caacatcacc cggttccaaa cccttcttgc ccttcatcga agctacctca
     7561 cgcctggcga cagtagtagt ggttggacag cgggcgcagc agcctactat gtgggatatt
     7621 tgcaaccacg cacctttctt ctcaaatata acgaaaacgg aacaattacc gatgccgtag
     7681 actgtgccct cgatcccctg tccgaaacta aatgcacact caagtccttc acagttgaga
     7741 aaggcatata ccaaaccagt aacttccgcg tccaacccac cgaatccatt gttcgattcc
     7801 ctaacatcac caatttgtgt cctttcggtg aagtatttaa tgccactcga tttgcatcag
     7861 tttatgcctg gaatcgcaag cgaatatcaa attgtgttgc agattatagt gtcctgtata
     7921 atagcgcatc attcagcact tttaagtgtt atggggtcag ccctacgaaa ttgaatgact
     7981 tgtgttttac caacgtttac gcagattctt tcgtcatacg cggcgacgaa gtccgacaga
     8041 ttgcaccggg gcaaaccgga aagatagcag actataatta taagttgccg gatgacttca
     8101 ccggctgcgt tatagcatgg aactccaata atctcgattc aaaagtcggc ggcaactata
     8161 attatcttta ccgcctgttt cgcaagtcaa atctgaagcc ctttgaacgt gacatctcca
     8221 ccgaaattta tcaagctggc agcaccccat gtaatggagt ggaagggttt aactgttatt
     8281 ttccccttca atcatacgga tttcaaccaa caaatggggt gggctatcaa ccatatcggg
     8341 tggtagtctt gagctttgag ctgctccacg caccggctac agtttgcgga ccgaagaaaa
     8401 gcactaacct ggtgaagaat aaatgtgtga acttcaactt taatgggctc accggaacag
     8461 gggtgctgac agaatcaaac aagaaatttc tcccttttca acaatttggt agagacattg
     8521 ccgataccac agatgccgtc cgcgatccgc aaacactgga aatcctcgat ataacaccct
     8581 gtagcttcgg cggggtcagc gtgataactc ccggaacgaa cacatccaac caagtggctg
     8641 tcctgtatca agatgtcaac tgtaccgaag tccctgtggc gatacacgcc gaccaactga
     8701 cacccacctg gagagtctac tcaacaggtt ccaatgtctt tcaaactaga gcgggttgtc
     8761 tcattggcgc tgagcatgtc aataattcat acgaatgtga tattcctatt ggggcaggaa
     8821 tctgtgcgtc ttatcaaact caaacgaact caccacggcg cgctcgctca gtagccagcc
     8881 agtccataat cgcctatacc atgagtctcg gggctgagaa ctccgtggct tattcaaaca
     8941 attccatcgc aatcccaact aactttacca ttagcgttac cacggaaatc ttgcccgtca
     9001 gcatgactaa gacgagcgta gactgtacga tgtatatctg tggggattcc accgagtgta
     9061 gcaacctctt gcttcaatac gggtctttct gtactcaact caaccgagca ttgaccggaa
     9121 ttgcggtcga acaagacaag aatacccaag aggtgttcgc acaagtaaag caaatataca
     9181 aaacgccgcc aatcaaagat tttggcgggt ttaattttag ccaaatactg ccagacccat
     9241 ctaagccttc aaaacgcagc tttatcgagg atctgctgtt caacaaagtt acgttggctg
     9301 acgcgggttt cattaagcag tatggggatt gtttgggaga cattgcggcc cgcgacctta
     9361 tctgtgctca aaaattcaat gggttgacag ttctgccccc tctgctcact gatgagatga
     9421 ttgcacagta tacatcagca ttgttggcag gaaccattac atccggctgg acttttggcg
     9481 cgggtgcggc ccttcaaata cctttcgcta tgcaaatggc ttacagattc aatgggattg
     9541 gtgtcacgca aaatgtattg tatgaaaatc agaagctgat cgctaatcaa tttaacagcg
     9601 cgattggcaa gatccaagat tcactgtcaa gcacagcgtc cgccctcggc aaactccaag
     9661 atgttgtgaa tcaaaacgcc caggccctga ataccctcgt gaagcaactg agttctaact
     9721 tcggcgcaat ctccagcgtc ctcaacgaca tattgtccag acttgataag gtggaagccg
     9781 aggtgcaaat tgatcggctc atcacaggca ggctccagtc actccaaact tatgtgacac
     9841 aacagttgat ccgggctgct gagatacgag caagcgccaa cctggccgcc actaaaatgt
     9901 cagagtgcgt gctcgggcag tccaaacggg ttgacttttg tggcaagggc tatcacttga
     9961 tgtccttccc ccaatccgcc ccacatggag tggtctttct ccacgtcact tatgtccctg
    10021 ctcaagagaa gaactttacg acagctccag caatctgtca cgatggcaaa gcgcactttc
    10081 ccagggaagg cgttttcgtc agtaatggca cgcattggtt tgtgacccaa aggaatttct
    10141 atgaacccca aatcataacc actgacaata catttgtctc aggcaactgt gatgtggtca
    10201 ttgggatcgt gaacaacacg gtatatgatc ccctgcaacc cgagttggat agcttcaaag
    10261 aggaattgga taaatacttt aagaatcata cttcacccga tgttgatctc ggggacatat
    10321 caggaataaa tgcaagtgtc gtcaacattc agaaagagat tgatcgcctt aatgaggtcg
    10381 ccaagaactt gaatgaatct ttgatcgact tgcaagaact gggcaaatac gaacaatata
    10441 ttaaatggcc ctg
//
