LOCUS       sequence-447110-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike A67V for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 A67V (GTT)
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      390..978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     879..898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    1174..1398
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(1409..2203)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1520..1539)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2130..2149
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2217..2413)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2231..2366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    2458..2529
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(2574..2710)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(2740..2786)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    2901..3022
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2938..2957)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2992..3011
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    3075..3663
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3181..3192
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3590..3610
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     intron          7560..8035
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     promoter        complement(8169..8371)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8198..8218)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8373..8676
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    8757..8805
                     /note="synthetic polyadenylation signal"
     terminator      8823..8869
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    8874..8945
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9097..9233)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(9246..10037,10038..10106))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9869..9888
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tacctggcga taggcgctgc caagcaatag ccaaggcgca aggccggcca agagacttgc
       61 gatagttcgt tcgagaggta gcctgcaaga tggatcggac gaaaggtttg aagattttcc
      121 gacgagggcc ttggagagtt cggaaattta aatgcaacta tggatgaacg aaatagacag
      181 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
      241 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
      301 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
      361 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
      421 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      481 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
      541 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      601 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      661 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      721 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
      781 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      841 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      901 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      961 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1021 tggccttttg ctcacatgat ttaaatgaaa ctcgtaatcg gagaacgggt gcaatagcaa
     1081 ggaccagatc gaacttgact gacgcaattc tgagcgtagt cgaaacccag acaagtcgat
     1141 ctcgccaagt agctgacttg actggttaat taaccataga gcccaccgca tccccagcat
     1201 gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa
     1261 tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag
     1321 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
     1381 ggcaactaga aggcacaggg cttacatact agaagaactc gtcaagaagg cgatagaagg
     1441 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     1501 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     1561 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     1621 tcggcaagca ggcatcgccg tgggtcacga cgagatcctc gccgtcgggc atccgcgcct
     1681 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     1741 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     1801 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     1861 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     1921 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     1981 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctggagttca ttcagggcac
     2041 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     2101 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     2161 aagcggcggg agaacctgcg tgcaatccat cttgttcaat catgttggct acctcctttg
     2221 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
     2281 gcggcctcgg cctctgcata aataaaaaaa attagtcagc gatggggcgg agaatgggcg
     2341 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
     2401 taattgagat gcagatcgct taattaagtt tcgatagccc aaggtaacca atgagattag
     2461 ggagggaaga aagtttgatg aactacttct gacccctagt ggtgtccaga aaagaccatt
     2521 aaaggaatgt cttgatcgaa atcgagagat cgcctgcagg atagctgacg accttaagga
     2581 taaatttctg gtaaggagga cacgtatgga agtgggcaag ttggggaagc cgtatccgtt
     2641 gctgaatctg gcatatgtgg gagtataaga cgcgcagcgt cgcatcaggc atttttttct
     2701 gcgccaatgc cggtagatag caatccggcc cgaggggcac aaaaaacccc tcaagacccg
     2761 tttagaggcc ccaaggggtt atgctacgat accgatagag atgggccaat accacatttg
     2821 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2881 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2941 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3001 ccaaactcat caatgtatct tatcatgtct gtgagctgaa ggtacgctgt atctcagtca
     3061 gtcaagctag acaggcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag
     3121 gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg
     3181 attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca
     3241 ggcgagcagc catggaaagg acgtcagctt ccccgacaac accacggaat tgtcagtgcc
     3301 caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc
     3361 aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca
     3421 accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg
     3481 ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc
     3541 catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa
     3601 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
     3661 attaggcctg cggatccaga aatcgattgt atcagtcagt cagtgcagga ggagacaact
     3721 tctagaggat cctttattta caacaagatc cgcaactgca acatcctttc aagcaggaac
     3781 aacagctagt catacagcac aacataattg tgaccataac gatagctata agtccggcga
     3841 taaagcccaa ccaaatgtac cagggccatt taatatattg ttcgtatttg cccagttctt
     3901 gcaagtcgat caaagattca ttcaagttct tggcgacctc attaaggcga tcaatctctt
     3961 tctgaatgtt gacgacactt gcatttattc ctgatatgtc cccgagatca acatcgggtg
     4021 aagtatgatt cttaaagtat ttatccaatt cctctttgaa gctatccaac tcgggttgca
     4081 ggggatcata taccgtgttg ttcacgatcc caatgaccac atcacagttg cctgagacaa
     4141 atgtattgtc agtggttatg atttggggtt catagaaatt cctttgggtc acaaaccaat
     4201 gcgtgccatt actgacgaaa acgccttccc tgggaaagtg cgctttgcca tcgtgacaga
     4261 ttgctggagc tgtcgtaaag ttcttctctt gagcagggac ataagtgacg tggagaaaga
     4321 ccactccatg tggggcggat tgggggaagg acatcaagtg atagcccttg ccacaaaagt
     4381 caacccgttt ggactgcccg agcacgcact ctgacatttt agtggcggcc aggttggcgc
     4441 ttgctcgtat ctcagcagcc cggatcaact gttgtgtcac ataagtttgg agtgactgga
     4501 gcctgcctgt gatgagccga tcaatttgca cctcggcttc caccttatca agtctggaca
     4561 atatgtcgtt gaggacgctg gagattgcgc cgaagttaga actcagttgc ttcacgaggg
     4621 tattcagggc ctgggcgttt tgattcacaa catcttggag tttgccgagg gcggacgctg
     4681 tgcttgacag tgaatcttgg atcttgccaa tcgcgctgtt aaattgatta gcgatcagct
     4741 tctgattttc atacaataca ttttgcgtga caccaatccc attgaatctg taagccattt
     4801 gcatagcgaa aggtatttga agggccgcac ccgcgccaaa agtccagccg gatgtaatgg
     4861 ttcctgccaa caatgctgat gtatactgtg caatcatctc atcagtgagc agagggggca
     4921 gaactgtcaa cccattgaat ttttgagcac agataaggtc gcgggccgca atgtctccca
     4981 aacaatcccc atactgctta atgaaacccg cgtcagccaa cgtaactttg ttgaacagca
     5041 gatcctcgat aaagctgcgt tttgaaggct tagatgggtc tggcagtatt tggctaaaat
     5101 taaacccgcc aaaatctttg attggcggcg ttttgtatat ttgctttact tgtgcgaaca
     5161 cctcttgggt attcttgtct tgttcgaccg caattccggt caatgctcgg ttgagttgag
     5221 tacagaaaga cccgtattga agcaagaggt tgctacactc ggtggaatcc ccacagatat
     5281 acatcgtaca gtctacgctc gtcttagtca tgctgacggg caagatttcc gtggtaacgc
     5341 taatggtaaa gttagttggg attgcgatgg aattgtttga ataagccacg gagttctcag
     5401 ccccgagact catggtatag gcgattatgg actggctggc tactgagcga gcgcgccgtg
     5461 gtgagttcgt ttgagtttga taagacgcac agattcctgc cccaatagga atatcacatt
     5521 cgtatgaatt attgacatgc tcagcgccaa tgagacaacc cgctctagtt tgaaagacat
     5581 tggaacctgt tgagtagact ctccaggtgg gtgtcagttg gtcggcgtgt atcgccacag
     5641 ggacttcggt acagttgaca tcttgataca ggacagccac ttggttggat gtgttcgttc
     5701 cgggagttat cacgctgacc ccgccgaagc tacagggtgt tatatcgagg atttccagtg
     5761 tttgcggatc gcggacggca tctgtggtat cggcaatgtc tctaccaaat tgttgaaaag
     5821 ggagaaattt cttgtttgat tctgtcagca cccctgttcc ggtgagccca ttaaagttga
     5881 agttcacaca tttattcttc accaggttag tgcttttctt cggtccgcaa actgtagccg
     5941 gtgcgtggag cagctcaaag ctcaagacta ccacccgata tggttgatag cccaccccat
     6001 ttgttggttg aaatccgtat gattgaaggg gaaaataaca gttaaaccct tccactccat
     6061 tacatggggt gctgccagct tgataaattt cggtggagat gtcacgttca aagggcttca
     6121 gatttgactt gcgaaacagg cggtaaagat aattatagtt gccgccgact tttgaatcga
     6181 gattattgga gttccatgct ataacgcagc cggtgaagtc atccggcaac ttataattat
     6241 agtctgctat ctttccggtt tgccccggtg caatctgtcg gacttcgtcg ccgcgtatga
     6301 cgaaagaatc tgcgtaaacg ttggtaaaac acaagtcatt caatttcgta gggctgaccc
     6361 cataacactt aaaagtgctg aatgatgcgc tattatacag gacactataa tctgcaacac
     6421 aatttgatat tcgcttgcga ttccaggcat aaactgatgc aaatcgagtg gcattaaata
     6481 cttcaccgaa aggacacaaa ttggtgatgt tagggaatcg aacaatggat tcggtgggtt
     6541 ggacgcggaa gttactggtt tggtatatgc ctttctcaac tgtgaaggac ttgagtgtgc
     6601 atttagtttc ggacagggga tcgagggcac agtctacggc atcggtaatt gttccgtttt
     6661 cgttatattt gagaagaaag gtgcgtggtt gcaaatatcc cacatagtag gctgctgcgc
     6721 ccgctgtcca accactacta ctgtcgccag gcgtgaggta gcttcgatga agggcaagaa
     6781 gggtttggaa ccgggtgatg ttgatgccaa tcggcaagtc cacgaggggt tccaacgcgg
     6841 agaagccttg agggagatcc cgaaccagat ttatcggggt atgcttgcta taaattttga
     6901 agtagccgtc tatattctta aacacaaatt cgcggagatt cttgaagttg ccttgcttcc
     6961 cttcaagatc catgagaaat ggttgggaaa cgtactcaaa agtacagttg ttagcggaac
     7021 tgtaaacgcg gaactctgac tccatccaag acttgttatt cttatgataa taaaccccga
     7081 gaaagggatc gttacaaaat tgaaattcac agactttgat gacgacattg gtggcgttgt
     7141 tcacaatgag gagagactgt gtcttggagt ccagggtggt gccaaatatc catccccgga
     7201 taatgttgga cttctccgtt gaggcaaagt atacgccatc gttaaatggc aagacgggat
     7261 tatcaaatcg tttagttccg ttggtgccag aaacgtggat aacatggaac caggtgacat
     7321 ttgagaagaa tggcaggaaa aggtcttgag tagaatgaag gacactggac ctaaagactt
     7381 tatcgggata gtatactcca cgagtaaaag agttcgtgta agcgggtggg agctgcgttc
     7441 tagttgtgag gttcacgcat tgggaagaaa cgaggggcag gagaacgagg aacacaaaca
     7501 tggcggccgc ctttgccaaa gtgatgggcc agcacacaga ccagcacgtt gcccaggagc
     7561 tgtgggagga agataagagg tatgaacatg attagcaaaa gggcctagct tggactcaga
     7621 ataatccagc cttatcccaa ccataaaata aaagcagaat ggtagctgga ttgtagctgc
     7681 tattagcaat atgaaacctc ttacatcagt tacaatttat atgcagaaat atttatatgc
     7741 agaaatattg ctattgcctt aacccagaaa ttatcactgt tattctttag aatggtgcaa
     7801 agaggcatga tacattgtat cattattgcc ctgaaagaaa gagattaggg aaagtattag
     7861 aaataagata aacaaaaaag tatattaaaa gaagaaagca ttttttaaaa ttacaaatgc
     7921 aaaattaccc tgatttggtc aatatgtgta ccctgttact tctccccttc ctatgacatg
     7981 aacttaacca tagaaaagaa ggggaaagaa aacatcaagg gtcccataga ctcaccctga
     8041 agttctcagg atccgagctc ggtaccacat gtaagcttcg aggggaggct ggatcggtcc
     8101 cggtgtcttc tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc
     8161 actaaaccag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
     8221 aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
     8281 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
     8341 cgcccattga tgtactgcca aaaccgcatc agcatggtaa tagcgatgac taatacgtag
     8401 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     8461 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     8521 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     8581 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     8641 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     8701 actaatgacc ccgtaattga ttactagatc tggctacact cgatcagcag ttagataata
     8761 aaatcgctat ccatcgaaga tggatgtgtg ttggtttttt gtgtgtgtaa cgcaacgatt
     8821 gatagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttga attgagaggt
     8881 agctgaagct gctgttatga ccactagagg gaagaagata cctgtggagc taatggtcca
     8941 agatgtcggg agccgcgatc gctgccaaaa aggtaagctg acgatagctg acgagattcg
     9001 atgcccgacg agcaaatgag cgccacggct gttcgcgtaa gctcgaaacg acggagattc
     9061 gatagagcca gagtcgagtt taaacgattc gggatcttaa ggataaattt ctggtaagga
     9121 ggacacgtat ggaagtgggc aagttgggga agccgtatcc gttgctgaat ctggcatatg
     9181 tgggagtata agacgcgcag cgtcgcatca ggcatttttt tctgcgccaa tgcacctaga
     9241 atcggttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9301 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9361 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9421 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9481 atccagtcta tcaactgttg ccgggaagct agagtaagta gttcgccagt taatagttta
     9541 cgcaaggttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9601 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9661 aaagcggtta gctccttcgg tcctccaatc gttgtcagaa gtaagttggc cgcagtgtta
     9721 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9781 ttttctgtga ctggtgaata ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9841 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9901 gtgctcatca ttggaaagcg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9961 agatccagtt cgatgtaacc cactcgcgca cccaactgat cttcagcatc ttttactttc
    10021 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10081 gcgacacgga aatgttgaat actcatgcga aagcctcctg ctgatcctgt ctcttgatag
    10141 atgtttgcaa aagctaggac tcgattatac gttgaccgat cagactatca atatctaggt
    10201 ttaaacagat cggtacgctg ccgtgaccag gcttgagtac aatatctgac gattggactt
    10261 cctgggtccc aagctggatt ccgactggct gactgccaag caacgatatg gatcgtcgag
    10321 actcgcactg agaggatcgt tctcggtaca ccaggcgcgc cccgatttcg atattcctcg
    10381 gatctcggat agatcgggat tgaggatgta ggttgaactt ctctcgggtg atcccgatct
    10441 cggatattcg ggattggagt aa
//
