LOCUS       sequence-44712-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Nakashima N, Noguchi E, Nishimoto T
  TITLE     Saccharomyces cerevisiae putative G protein, Gtr1p, which forms 
            complexes with itself and a novel protein designated as Gtr2p, 
            negatively regulates the Ran/Gsp1p G protein cycle through Gtr2p.
  JOURNAL   Genetics. 1999 Jul . 152(3):853-67.
  PUBMED    10388807
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            1999 Jul . 152(3):853-67."
COMMENT     Sequence Label: pAS404
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             143..583
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             599..625
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             605..631
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(700..719)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(701..719)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(729..746)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(729..745)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(738..760)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ataaatagac ctgcaattat taatcttttg tttcctcgtc attgttctcg ttccctttct
       61 tccttgtttc tttttctgca caatatttca agctatacca agcatacaat caactccaag
      121 cttgaagcaa gcctcctgaa agatgaagct actgtcttct atcgaacaag catgcgatat
      181 ttgccgactt aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa
      241 gaacaactgg gagtgtcgct actctcccaa aaccaaaagg tctccgctga ctagggcaca
      301 tctgacagaa gtggaatcaa ggctagaaag actggaacag ctatttctac tgatttttcc
      361 tcgagaagac cttgacatga ttttgaaaat ggattcttta caggatataa aagcattgtt
      421 aacaggatta tttgtacaag ataatgtgaa taaagatgcc gtcacagata gattggcttc
      481 agtggagact gatatgcctc taacattgag acagcataga ataagtgcga catcatcatc
      541 ggaagagagt agtaacaaag gtcaaagaca gttgactgta tcgccggaat tcatggctta
      601 cccatacgat gttccagatt acgctagctt gggtggtcat atggccatgg aggccccggg
      661 gatccgtcga cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac
      721 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      781 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      841 accgatcgcc
//
