LOCUS       sequence-447144-        4678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible 5' entry clone containing the Human Claudin 5 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: p5E-hs_Cldn5 (JDW 1120)
FEATURES             Location/Qualifiers
     source          1..4678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     70..87
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(246..273)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(365..451)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     500..522
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     514..531
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     515..531
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    571..666
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        668..686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          2433..2565
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(2630..2646)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2630..2646)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(2683..2701)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2702..2825
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2898..2916)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2921..2937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2921..2937)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3039..3058)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3050..3859
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3117..3136)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4006..4594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4495..4514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
       61 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      121 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      181 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
      241 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
      301 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
      361 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
      421 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
      481 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
      541 gggcccgagt taacgctacc atggagctcc aaataatgat tttattttga ctgatagtga
      601 cctgttcgtt gcaacaaatt gataagcaat gcttttttat aatgccaact ttgtatagaa
      661 aagttggtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga
      721 caacccttaa tgaattcgag ctcctaggcc ggcccttgcc ttcagaacct ccccacacta
      781 ggtgagccag acgctggcct tatctcattt accatctcag agccatctga agggggagaa
      841 gggaaccggg ccccaggagg gagaaagtca tcaaacctcc cacatctgtg accagcctca
      901 gtgccatact ttttctatgg agggccctgt ccaatggaac tgagcacaga ccagataaaa
      961 gaactgggca cccagtggcc tcagtccagg gcctggagtt caaactttac tggaaacaaa
     1021 ggggccgaga gagactgggg aaagaactac tagaaagggg ctggtgcccc catggggctg
     1081 tgggttttgg agccgcgtgc ccccacctga gcctcagggg gcccggagtg tccacaccag
     1141 tggacctttc gagaaatggc tgggccattg tgcagaagaa tgcccggaaa tcccgcgcct
     1201 ccctcctcca gcaaggatgg gggctcttcc tcctggccag gaaactccaa gttggcttcc
     1261 ggagggtggc ctgggggctg gggtgccagg gacaccatcg ccactggtgg gagggcaggg
     1321 cacagcccct ccgtgtccct ttgtctctcc tgtctgaagg ccagagcagg ctgctaggcc
     1381 tggggccacc actgcccctg ggtgctacac ccagtgtgct gggtcactgg gaacttcctg
     1441 aagtggtgtc acctgaactg ggcccccaag gatggggtgc gggcagtacc gcaggaagag
     1501 gagcagcccc tgtgaagatt gagaggtctg ggaagcccct gcggcttggg agagtggggg
     1561 tcgccaggca gggggaaagc ccctgtgcca ccgctttttg ccagagactc aggctccaga
     1621 gaggcagtga gtggcatggg gggtgaggct ggggccctgg gcctgacctc cacacgcctg
     1681 cctggcctct ctgtttgcca tgggatgaga gagacagtgc tgggactcag agcggggctg
     1741 gagagtgaga gtgcgagaaa gggcctgggt ggggcttgga ccccggggcg ggctttctgg
     1801 agagcccccc tacgagggcc tctacggcgg tgacggggtg gggggcttct gcaaaccttg
     1861 gtcagggaag tggagctggc tcgagtggaa gagaccaccc ggctcagtcg gggatgtggg
     1921 agtggactgg gtggtgcaga ctgggggtcg agcgccttct gaagtgacgg ggccgggacg
     1981 cgcagggagg cggcccaaga agcgcgccct aggccagccc agaatgcgct cggccgcgac
     2041 taggacaacg gcgggtgggg ctgggggcgg ctgccgggcg gggagcggtc ccgcgccctc
     2101 agctacccct caagagccgt tgtttcccta acttcagctg ccagaggctc tgtgattggc
     2161 tgcggcacga tgacccgcgc acggattggc tgcttcgggc cggggggccg ggcccggggg
     2221 acagaatccg cccccgaacc ttcaaagagg gtaccccccg gcaggagctg gcagacccag
     2281 gaggtgcgac agacccgcgg ggcaaacgga ctggggccaa gagccgggag cgcgggcgca
     2341 aaggcaccag ggcccgccca gggcgccgcg cagcacggcc ttgggggttc tgcgggcctt
     2401 cgggtgcgcg tctcgcctct agccggcgcg ccgtaagtat caaggttaca agacaggttt
     2461 aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg
     2521 cacctattgg tcttactgac atccactttg cctttctctc cacaggaagt tcctattccg
     2581 aagttcctat tcttcaaaaa gcttgatatc gaattcctgc agcccggggg atccactagt
     2641 tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
     2701 tcaagtttgt acaaaaaagt tgaacgagaa acgtaaaatg atataaatat caatatatta
     2761 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatgcagtca
     2821 ctatgaacca actacttaga tggtattagt gacctgtaga attcgagctc tagagctgca
     2881 gggcggccgc gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2941 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     3001 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     3061 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     3121 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     3181 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     3241 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     3301 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     3361 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     3421 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     3481 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     3541 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     3601 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     3661 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     3721 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     3781 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     3841 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     3901 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     3961 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4021 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4081 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4141 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4201 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4261 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4321 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4381 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4441 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4501 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4561 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4621 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgat
//
