LOCUS       sequence-447145-        2877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible 5' entry clone containing the Human minimal ICAM2
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: p5E-hs_ICAM_short (JDW 1046)
FEATURES             Location/Qualifiers
     source          1..2877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     67..84
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(243..270)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(362..448)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     497..519
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     511..528
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     512..528
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    568..663
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        665..683
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(879..897)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    898..1021
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1094..1112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1117..1133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1117..1133)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1235..1254)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1246..2055
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1313..1332)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2202..2790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2691..2710
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
       61 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
      121 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
      181 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
      241 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
      301 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
      361 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      421 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
      481 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
      541 cccgagttaa cgctaccatg gagctccaaa taatgatttt attttgactg atagtgacct
      601 gttcgttgca acaaattgat aagcaatgct tttttataat gccaactttg tatagaaaag
      661 ttggtaatac gactcactat agggcgaatt gggtaccggg ccccccctcg aggtagaacg
      721 agctggtgca cgtggcttcc caaagatctc tcagataatg agaggaaatg cagtcatcag
      781 tttgcagaag gctagggatt ctgggccata gctcagacct gcgcccacca tctccctcca
      841 ggcagccctt ggccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattcaa
      901 gtttgtacaa aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
      961 agattttgca taaaaaacag actacataat actgtaaaac acaacatatg cagtcactat
     1021 gaaccaacta cttagatggt attagtgacc tgtagaattc gagctctaga gctgcagggc
     1081 ggccgcgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     1141 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     1201 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1261 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1321 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     1381 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     1441 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     1501 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     1561 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     1621 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     1681 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     1741 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     1801 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     1861 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     1921 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     1981 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2041 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     2101 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     2161 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2221 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2281 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2341 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2401 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2461 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2521 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2581 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2641 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2701 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2761 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2821 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattct
//
