LOCUS       sequence-447202-        5164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible 5' entry clone containing the Murine Cdh5 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: p5E-mmCdh5pro (JDW 1239)
FEATURES             Location/Qualifiers
     source          1..5164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    3210..3333
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3405..3424)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3406..3424)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3429..3445)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3429..3445)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3547..3566)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3558..4367
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3625..3644)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4514..5102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5003..5022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgct cgaggtcgac tctagtagca gaaacaaggt
      721 cctctggaag agcaactgat gctcttaggt actgaagcat catcctgccc cagagaccac
      781 tcgcatatga agcacacata ttcagtctgc cttacttgtg ttaatgattg ccagtgtccc
      841 tctgacctcc tagccctgaa aagtgtggcc tgaaggtcat ttcagagacg gggagagctg
      901 ctcagagaag ccaatcggcg agtctaggac acacagacag gatctagtcc cagagttcgc
      961 tagcctaggt gagcgtcccc tggcccctta taccacttcc ttctccagct tgcatctaat
     1021 ctgctctggc agaccatcgt gtttcctgtc ttcctggcag cctccagcac gctcagtgct
     1081 actccctgcg catgcgccct cctcccagta ccttctctga ctccagtggg cttggagtgc
     1141 gaggaggaag ggtgaggaag gggtgaaatc aggtattgga tccacagggg gtctgaagag
     1201 cactagcctg gccttttggg actgaacttc tgctatgaag acctccactg ccatccctgg
     1261 agtccggggc acatccaagg cttgcctgtc catcgtttac tgtttacaga tgacaacaat
     1321 gactgtgttc ggggcagaaa tatccaccag ggctagagta caaaaggagt ttgcattgat
     1381 ggccggacag gccctgtccc tggcagcctg ccagcgctga gtatgagacc cagcgggaag
     1441 tgctaccctg gcagacgtgt ccactgagta cacagaccac caaggcaggc agctctcggg
     1501 gaagctgtct atgctgggcc agcccacctt gagggcaggg aacagaacag attgtggcag
     1561 agaggaaaat gtggagcttc tgtttgttca cagacacacg cactcgccca cgcacgcacg
     1621 cacgcacgca cgcacgcacg aatgcacgca cgcagtagtt gaatgctatg gattccgctc
     1681 agagctgaga acagccccag cgacagttcc ctggcctctc tccttactct gatgtcctca
     1741 tctgtcttca catggtctca ggacgctaat actccatcct aatgtacact cctttccctg
     1801 ggcctccgtt ccagttcagt tctcagagga cctggaggga gtgattggct acaccaactt
     1861 tgctttcgtt caccaagccc atgtctctac ttgggtgtct aatgggcatc tccaacatta
     1921 cctaccccaa acagaaaacc ctttcttccc cccaccacac cccaccctac ccccacagta
     1981 ttttctccat gcccggaaag atctgctctc ttatggtccc tctttgcctc actgaaaagc
     2041 aggacaagtt ggggacttcc caaactttta tgcatgaaga aacccaggca atttgccaaa
     2101 aggtacactc tgggggtctg tcatttactc tgagccagaa ccctgaaatt tttactaacc
     2161 catcacataa tgaatgaaga gaatcttttt cttttttttt ttttttcttt ttttttggtt
     2221 tttcgagaca gggtttctct gtatagccct ggctatcctg gaacacactc tgtagaccag
     2281 gctggcctcg aactcagaaa tccacctgcc tctgcctccc gagtgctggg attaaaggcg
     2341 tgcgccacca cgcctggctg aatgaagaga atcttgacct catctcccca gcctcttggt
     2401 cctgagggac cctggtctac ctactgcttt gctgtcttct tagctcttct tacttttttg
     2461 ctgactcaga cctatggcta tctccattat acagatgagg agactgaggc atggatccct
     2521 ggttggtcca tggtcacgtg aagcccatca cccagtattt gtaaagtgag atgggccagg
     2581 ctggtacctt ggaactgaaa ctcacactgc cctacctgga agaatctgac aggcaaaatc
     2641 tgctgctgaa agtgattgtc tgtcacgttt ctcagctgcc cgactctgag aactccacag
     2701 ccccctttcg ttccaccata ctacagagtc gccacggaaa gccggctctg tggagaagct
     2761 gaggtagctg ggtttctgtc tgggttactc tgtccagcga ggaaacaagt accttagacc
     2821 cactaagcct ctgctttctg aactgtaaag tgggggatat gacacctgcc tcccagggat
     2881 ggctgaatgc tctggcagaa gcttagagcc cccacagcta cccctaggct cacagctcct
     2941 ccgatgagac ctagaattga ggtatgagtt gaatacccca ggcaggtcca aggcttccac
     3001 gggcccaggc tgaccaagct gaggccgccc accgtagggc ttgcctatct gcaggcagct
     3061 cacaaaggaa caataacagg aaaccatccc gaggggaagt gggccagggc cagttggaaa
     3121 acctgcctcc ctcccagcct gggtgtggct cccctctccc ctcctgaggc aatcaactgt
     3181 gctctccaca aagctcggcc ctgaattctc aagtttgtac aaaaaagttg aacgagaaac
     3241 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
     3301 atactgtaaa acacaacata tgcagtcact atgaaccaac tacttagatg gtattagtga
     3361 cctgtagaat tcgagctcta gagctgcagg gcggccgcga tatcccctat agtgagtcgt
     3421 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     3481 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     3541 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     3601 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     3661 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     3721 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     3781 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     3841 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     3901 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     3961 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     4021 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     4081 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     4141 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     4201 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     4261 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     4321 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     4381 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     4441 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     4501 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4561 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4621 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4681 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4741 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4801 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4861 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4921 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4981 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5041 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5101 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5161 tgtt
//
