LOCUS       sequence-44721-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Brent AE, Tabin CJ
  TITLE     FGF acts directly on the somitic tendon progenitors through the Ets 
            transcription factors Pea3 and Erm to regulate scleraxis expression.
  JOURNAL   Development. 2004 Aug . 131(16):3885-96.
  PUBMED    15253939
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2004 Aug . 131(16):3885-96."
COMMENT     Sequence Label: pBS cFgfr1 (CT#220)
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(562..578)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(563..579)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(608..628)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(608..626)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(647..663)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(647..663)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(660..682)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    671..687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(695..701,702..719,720..725))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    740..761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(878..895)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgctctgact gtggatcccc cgggctgcag gaattcgatg gggtacaagg tccgctatgc
       61 cacctggagc atcatcatgg actcggtggt gccatcagat aagggcaact acacgtgcat
      121 cgtggagaac aaatacggga gcatcaacca cacctaccag ctggatgtcg tggagcgctc
      181 cccgcatcgg cccatcctgc aggcagggct ccccgccaac aaaacggtgg ccctgggcag
      241 caacgtggag tttgtctgca aggtctacag cgacccgcag ccccacatcc agtggctgaa
      301 acacatcgag gtgaacggca gcaagatcgg ccccgacaac ttgccctacg tgcagatcct
      361 gaagacggct ggcgttaaca cgacagacaa agagatggaa gtccttcact taaggaatgt
      421 ctcatttgag gatgctgggg agtatacatg tttggcgggt aattctattg ggatctccca
      481 tcactctgca tggttgacag ttctcgaagc tactgagcag tcaccagcca tgatgacgga
      541 tccgtcgaca atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccagct
      601 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
      661 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccggg agcataaagt
      721 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      781 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      841 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      901 cggtct
//
