LOCUS       sequence-447210-       11540 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT5G42765.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 11540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT5G42765-mKOk
FEATURES             Location/Qualifiers
     source          1..11540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1387..1411)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2392..2410)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2393..2410)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2439..2448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2478..2497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2479..2497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2504..2521)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2504..2520)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2513..2535)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2678..2861
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(2862..2903,2904..2912,2913..3683)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2943..2962)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3553..3572
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4308..4560
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4653..4677
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5198..5989
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6235..6823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6724..6743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6977..6994
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7009..7149
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7075..7094)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7235..7257
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7493..7687
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7753..8826)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9255..9884)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11215..11239
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11404..11425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11440..11445,11446..11463,11464..11470)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11478..11494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11483..11505
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11502..11518
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11502..11518
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat ggctcgtctt atcccaattt
      721 ctcaaccttt cttctcttca aacactaaac ctccaccacc accaccaatc gccgccgcac
      781 aaatctccga ccacctgcca cagattatct ctcgccgaga cgccaccgtt cttctctcct
      841 ccgccacttc tcttctcctc actctctccc ccaccaatcc cgctttcgcc ttctccttag
      901 gaatctcagg acctaaagag tggcttaaag atcagaagaa gaagtcttca agattcttac
      961 ttgctcccat tgatgcaact cgtcaagctc ttcaatctgc ttatctttcg ttaactagtg
     1021 aatctgatta tacagagaaa gatttggaga atctgcagaa tctgtttaag tcttctgcaa
     1081 gagattgtgt tcctaaagaa agaagctctc ttgttgattt tcaatctaaa tccggagttg
     1141 aggtttgtac atttaagctt gttgtgaaga atgctgcttc attacttgat gataaggatc
     1201 cagttaaatt agaagcagag aatatacttg atgatcttgt taggtctttc ggttccctta
     1261 tcgtgttgac gaatggggtt gatatgaatc ttccttctga tagaaaaaaa gttgcagatg
     1321 gggttacaga gactatctct tatcttgaca aatttgagaa gggtgtcaaa gactgtcttg
     1381 agattcaccc agctttcttg tacaaagtgg tgatgggacg tccgcggaga tctacgcgtg
     1441 tcgactcgag atatccaact agtatggtga gcgttatcaa gcctgagatg aagatgaggt
     1501 actacatgga cggaagcgtg aacggacacg agttcactat tgaaggcgaa ggtactggaa
     1561 ggccttacga gggacatcaa gagatgactc tcagagtgac catggctgaa ggtggaccta
     1621 tgcctttcgc tttcgatctc gtgtctcacg tgttctgcta cggacacaga gtgttcacta
     1681 agtaccctga agagatcccg gactacttca agcaagcttt ccctgaggga ctctcttggg
     1741 agagatcttt ggaattcgag gacggtggat ccgcttctgt gtctgctcat atctctctca
     1801 ggggcaacac cttctaccac aagagcaagt ttaccggggt gaacttccct gctgatggac
     1861 cgatcatgca gaaccagtct gttgattggg agccgtctac tgagaagatc accgcttctg
     1921 atggtgtgct caagggtgat gtgaccatgt accttaagct cgaaggtggt ggaaaccaca
     1981 agtgccagtt caagactacc tacaaggccg ccaaagaaat cctcgagatg cctggtgatc
     2041 actacatcgg acataggctc gtgagaaaga ccgagggaaa catcactgag caggttgagg
     2101 atgctgtggc tcattctcaa ttgttataag cggccatgct agagtccgca aaaatcacca
     2161 gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc
     2221 cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
     2281 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     2341 caaaatccag tgacctgcag gcatgcaagc tagcttacta gtgatgcata ttctatagtg
     2401 tcacctaaat ctgcggccgc actagtgata tcccgcggcc atggcggccg ggagcatgcg
     2461 acgtcgggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     2521 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     2581 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     2641 gcagcctgaa tggcgaatgg aaattgtaag cgttaatggg tttctggagt ttaatgagct
     2701 aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc
     2761 tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc
     2821 aattagagtc tcatattcac tctcaatcca aataatctgc aatggcaatt accttatccg
     2881 caacttcttt acctatttcc gcccggatcc gggcaggttc tccggccgct tgggtggaga
     2941 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     3001 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     3061 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     3121 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     3181 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     3241 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     3301 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     3361 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     3421 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     3481 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     3541 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     3601 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     3661 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     3721 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     3781 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     3841 gttcttcgcc caccccgatc caacacttac gtttgcaacg tccaagagca aatagaccac
     3901 gaacgccgga aggttgccgc agcgtgtgga ttgcgtctca attctctctt gcaggaatgc
     3961 aatgatgaat atgatactga ctatgaaact ttgagggaat actgcctagc accgtcacct
     4021 cataacgtgc atcatgcatg ccctgacaac atggaacatc gctatttttc tgaagaatta
     4081 tgctcgttgg aggatgtcgc ggcaattgca gctattgcca aaatcgaaat acccctcacg
     4141 catgcattca tcaatattat tcatgcgggg aaaggcaaga ttaatccaac tggcaaatca
     4201 tccagcgtga ttggtaactt cagttccagc gacttgattc gttttggtgc tacccacgtt
     4261 ttcaataagg acgagatggt ggagtaaaga aggagtgcgt cgaagcagat cgttcaaaca
     4321 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     4381 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     4441 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     4501 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     4561 gaccggcatg caagctgata attcaattcg gcgttaattc agtacattaa aaacgtccgc
     4621 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
     4681 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
     4741 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
     4801 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
     4861 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg
     4921 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     4981 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     5041 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     5101 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     5161 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
     5221 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     5281 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     5341 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     5401 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     5461 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     5521 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     5581 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     5641 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     5701 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     5761 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     5821 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     5881 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     5941 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     6001 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     6061 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     6121 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
     6181 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6241 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6301 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6361 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     6421 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6481 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6541 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6601 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6661 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6721 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6781 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6841 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6901 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6961 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     7021 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     7081 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     7141 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7201 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     7261 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     7321 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     7381 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     7441 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     7501 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     7561 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     7621 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     7681 atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     7741 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     7801 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     7861 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     7921 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     7981 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     8041 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     8101 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     8161 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     8221 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     8281 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     8341 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     8401 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     8461 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     8521 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     8581 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     8641 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     8701 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     8761 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     8821 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
     8881 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
     8941 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
     9001 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
     9061 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
     9121 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
     9181 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
     9241 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
     9301 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
     9361 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
     9421 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
     9481 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
     9541 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
     9601 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
     9661 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
     9721 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
     9781 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
     9841 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
     9901 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
     9961 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
    10021 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
    10081 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
    10141 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
    10201 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
    10261 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
    10321 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
    10381 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
    10441 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
    10501 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
    10561 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
    10621 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
    10681 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
    10741 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
    10801 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
    10861 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
    10921 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
    10981 cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact
    11041 atagcagcgg aggggttgga tcaaagtact ttaaagtact ttaaagtact ttaaagtact
    11101 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
    11161 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
    11221 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
    11281 atccaagctc aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat
    11341 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    11401 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    11461 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
    11521 gattacgaat tcgagctcgg
//
