LOCUS       sequence-44725-e         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Zhang G, Yanamala N, Lathrop KL, Zhang L, Klein-Seetharaman J, 
            Srinivas H
  TITLE     Ligand-independent antiapoptotic function of estrogen receptor-beta 
            in lung cancer cells.
  JOURNAL   Mol Endocrinol. 2010 Sep;24(9):1737-47. Epub 2010 Jul 21.
  PUBMED    20660297
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Endocrinol."; date: "2010-09"; volume: "24(9)"; pages: "1737-47. 
            Epub 2010 Jul 21"
COMMENT     Sequence Label: pcDNA Flag ERbeta
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             123..188
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgagc tcggatccta
      121 tggactacaa agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg
      181 atgacaagct tatggatata aaaaactcac catctagcct taattctcct tcctcctaca
      241 actgcagtca atccatctta cccctggagc acggctccat atacatacct tcctcctatg
      301 tagacagcca ccatgaatat ccagccatga cattctatag ccctgctgtg atgaattaca
      361 gcattcccag caatgtcact aacttggaag gtgggcctgg tcggcagacc acaagcccaa
      421 atgtgttgtg gccaacacct gggcaccttt ctcctttagt ggtccatcgc cagttatcac
      481 atctgtatgc ggaacctcaa aagagtccct ggtgtgaagc aagatcgcta gaacacacct
      541 tacctgtaaa cagagagaca ctgaaaagga aggttagtgg gaaccgttgc gccagccctg
      601 ttactggtcc aggttcaaag agggatgctc acttctgcgc tgtctgcagc gattacgcat
      661 cgggatatca ctatggagtc tggtcgtgtg aagga
//
