LOCUS       sequence-44726-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Unsal-Kacmaz K, Chastain PD, Qu PP, Minoo P, Cordeiro-Stone M, 
            Sancar A, Kaufmann WK
  TITLE     The human Tim/Tipin complex coordinates an Intra-S checkpoint 
            response to UV that slows replication fork displacement.
  JOURNAL   Mol Cell Biol. 2007 Apr . 27(8):3131-42.
  PUBMED    17296725
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Apr . 27(8):3131-42."
COMMENT     Sequence Label: pcDNA4-Chk1-Flag
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             356..379
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(408..425)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    414..638
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(771..790)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccaggatcc tcacagaacc cctggcagcg gttggtcaaa agaatgacac gattctttac
       61 caaattggat gcagacaaat cttatcaatg cctgaaagag acttgtgaga agttgggcta
      121 tcaatggaag aaaagttgta tgaatcaggt tactatatca acaactgata ggagaaacaa
      181 taaactcatt ttcaaagtga atttgttaga aatggatgat aaaatattgg ttgacttccg
      241 gctttctaag ggtgatggat tggagttcaa gagacacttc ctgaagatta aagggaagct
      301 gattgatatt gtgagcagcc agaaggtttg gcttcctgcc acactcgagg gtatggacta
      361 caaggacgat gacgataagt gagttaacgt ttaaacccgc tgatcagcct cgactgtgcc
      421 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      481 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      541 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
      601 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
      661 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      721 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      781 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaaatcggg
      841 ggctccnttt agggttccga tttagtgctt ttacggcacc tcgaccccc
//
